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trees



Registered: 02/08/09
Posts: 9,194
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Vaporizing H202 for container, chamber, glove box sterilization?
#22460109 - 11/01/15 12:40 AM (8 years, 2 months ago) |
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I don't know how stable H202 is when vaporized by a humidifier, maybe a chemist who knows can chime in?
I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
I know that H202 is not very stable and might decompose in the ultra sonic vaporizer?
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Edited by trees (11/01/15 01:52 AM)
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AquaticPuzzle
f@#ked up



Registered: 01/26/15
Posts: 424
Loc: US
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22460112 - 11/01/15 12:41 AM (8 years, 2 months ago) |
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Vaporizing H202 for glove box sterilization? [Re: AquaticPuzzle]
#22460131 - 11/01/15 12:49 AM (8 years, 2 months ago) |
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no, we dont'need the sab to be sterile we need it to be still, pumping air in would ruin that just get your sab wet and sanitize your jars and what not
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Psilosoulful

Registered: 09/05/14
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Re: Vaporizing H202 for glove box sterilization? [Re: AquaticPuzzle]
#22460134 - 11/01/15 12:52 AM (8 years, 2 months ago) |
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 As long as you have proper sterile techniques, like spraying down the entire box with soap water and letting it sit for a few minutes, not touching the rim of any exposed jars, not having your hands hover over any exposed plates, flame sterilizing, etc., your jars will be okay and you'll have a very low rate of contams.
Here's a good writeup on sterile techniques http://www.shroomery.org/forums/showflat.php/Number/21889950/fpart/all/vc/1
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: Psilosoulful]
#22460141 - 11/01/15 12:54 AM (8 years, 2 months ago) |
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Ok, but I still really want to know because it has more uses than just the glove box.
Will the hydrogen peroxide remain intact after vaporization. Can the vapor produced from hydrogen peroxide be used to sterilize a surface or air?
Edited by trees (11/01/15 12:55 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22460166 - 11/01/15 01:03 AM (8 years, 2 months ago) |
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i'm going on a hunch here but no, it breaks down fast in the presence of oxygen and light which is why it's in a dark bottle but wait fro someone else i guess
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Psilosoulful

Registered: 09/05/14
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22460173 - 11/01/15 01:05 AM (8 years, 2 months ago) |
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You don't even need the hydrogen peroxide, and definitely don't vaporize it, that's just silly. Use isopropyl alcohol to sanitize the surfaces of your jar lids. That's it. The rest is just making sure your tools are flame sterilized before using. Oh, and your SAB should be wet inside, you want contams to stick to the sides of the box.
Here's mine:
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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Re: Vaporizing H202 for glove box sterilization? [Re: cronicr]
#22460174 - 11/01/15 01:06 AM (8 years, 2 months ago) |
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I doubt you can even get it to boiling without exponentially speeding up the rate at which it turns to water.
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Arctic W. Fox

Registered: 09/23/14
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Re: Vaporizing H202 for glove box sterilization? [Re: cronicr]
#22460178 - 11/01/15 01:09 AM (8 years, 2 months ago) |
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H2O2 is unstable with light, heat, exposure to air and time - just like ozone.
Follow standardized procedures. No need to reinvent the wheel.
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TheMaster
PATIENCE


Registered: 07/13/13
Posts: 705
Loc: Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22460200 - 11/01/15 01:21 AM (8 years, 2 months ago) |
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https://en.m.wikipedia.org/wiki/Vaporized_hydrogen_peroxide
This is a common technique utilized in clean rooms and other applications. It poses serious risks and requires proper engineering to ensure safety.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22460353 - 11/01/15 01:43 AM (8 years, 2 months ago) |
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Wiki article suggests that it does work.
This application might be able to be used to help sterilize an occupied fruiting chamber if early signs of pathogens are present, or just routine prevention of pathogens. I'm not looking to just sterilize a glove box.
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Edited by trees (11/01/15 01:50 AM)
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22460356 - 11/01/15 01:45 AM (8 years, 2 months ago) |
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Quote:
Inocuole said: I doubt you can even get it to boiling without exponentially speeding up the rate at which it turns to water.
Its not boiling, its passing the h2o2 solution over a sonic vaporizer to produce a fine mist fog
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: trees]
#22460365 - 11/01/15 01:55 AM (8 years, 2 months ago) |
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Quote:
trees said: I don't know how stable H202 is when vaporized by a humidifier, maybe a chemist who knows can chime in?
I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
I know that H202 is not very stable and might decompose in the ultra sonic vaporizer?
Chemicals sanitize, heat and filtration sterilize.
There is no need for peroxide in this hobby outside of treating cobweb mold.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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PussyFart
Retired Cultivation Extrodinaire



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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: PussyFart]
#22460366 - 11/01/15 01:56 AM (8 years, 2 months ago) |
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Quote:
PussyFart said: I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
Chemicals sanitize, heat sterilizes.
There is no need for peroxide in this hobby outside of treating cobweb mold.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: PussyFart]
#22460369 - 11/01/15 01:56 AM (8 years, 2 months ago) |
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Quote:
I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
Chemicals sanitize, heat sterilizes.
There is no need for peroxide in this hobby outside of treating cobweb mold.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22460429 - 11/01/15 03:05 AM (8 years, 2 months ago) |
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PF what happened man?
Quote:
trees said:
Quote:
Inocuole said: I doubt you can even get it to boiling without exponentially speeding up the rate at which it turns to water.
Its not boiling, its passing the h2o2 solution over a sonic vaporizer to produce a fine mist fog
And you're gonna do that... in an area in your home?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22460446 - 11/01/15 03:22 AM (8 years, 2 months ago) |
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Quote:
Inocuole said: PF what happened man?
Cocaine......but what are you referring to?
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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blindingleaf
blue collar underworld


Registered: 07/19/13
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Loc: sub-surface unseen
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22460463 - 11/01/15 03:40 AM (8 years, 2 months ago) |
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-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Vaporizing H202 for glove box sterilization? [Re: blindingleaf]
#22460566 - 11/01/15 05:22 AM (8 years, 2 months ago) |
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Quote:
I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
Wouldn't it be easier to just boil some water and pipe the steam in to sterilize it if you wanted to do that? Hydrogen peroxide vapor can be dangerous. I also think all the moisture has to be removed from the air first to even produce it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Vaporizing H202 for glove box sterilization? [Re: Kizzle]
#22460620 - 11/01/15 06:11 AM (8 years, 2 months ago) |
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Hospitals use peroxide vapor to "sterilize" rooms. Though it's chemical it achieves near sterilization on already mechanically clean surfaces. It's not peroxide in any concentration you can buy and if you could you don't have the special fogger and if you did you would probably kill yourself. Peroxyacetic acid sanitizer achieves near sterilization on cleaned stainless steel surfaces but its also nasty shit.
Rely on technique not chemical bullshit. Quit trying to cut corners OP
In Japan back in the day they put a beaker of formaldehyde on a hot plate to fumigate a whole room to sanitize/sterilize it.
Edited by Trusted cuItivator (11/01/15 06:13 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22461064 - 11/01/15 08:55 AM (8 years, 2 months ago) |
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Quote:
PussyFart said:
Quote:
Inocuole said: PF what happened man?
Cocaine......but what are you referring to?
I meant your weird triple post where you somehow quoted yourself saying something you didn't say.
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461191 - 11/01/15 09:24 AM (8 years, 2 months ago) |
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This was obviously the wrong board to ask about vaporized h2o2. Nobody here has any knowledge on chemistry. Bunch of fucking retards.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22461202 - 11/01/15 09:29 AM (8 years, 2 months ago) |
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Quote:
trees said: This was obviously the wrong board to ask about vaporized h2o2. Nobody here has any knowledge on chemistry. Bunch of fucking retards.
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TheMaster
PATIENCE


Registered: 07/13/13
Posts: 705
Loc: Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461255 - 11/01/15 09:43 AM (8 years, 2 months ago) |
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Quote:
PussyFart said:
Quote:
trees said: I don't know how stable H202 is when vaporized by a humidifier, maybe a chemist who knows can chime in?
I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
I know that H202 is not very stable and might decompose in the ultra sonic vaporizer?
Chemicals sanitize, heat and filtration sterilize.
There is no need for peroxide in this hobby outside of treating cobweb mold.
At the proper concentrations and with the appropriate engineering, hydrogen peroxide is indeed a sterilant. http://www.cdc.gov/hicpac/Disinfection_Sterilization/13_02sterilization.html Microbicidal Activity. This process has the ability to inactivate a broad range of microorganisms, including resistant bacterial spores. Studies have been conducted against vegetative bacteria (including mycobacteria), yeasts, fungi, viruses, and bacterial spores469, 721, 856, 881-883, 890-893. Like all sterilization processes, the effectiveness can be altered by lumen length, lumen diameter, inorganic salts, and organic materials469, 721, 855, 856, 890, 891, 893.
Trees, you're probably not going to be able to accomplish your goal with over the counter hydrogen peroxide. At that low of concentration the sporicidal activity will be greatly dimished. Peracetic acid in combination with low concentration hydrogen peroxide would boost efficacy as a sterilant. Also, this technique also requires you initiate free radical generation of the hydrogen peroxide. It seems a bit out of reach for what most people have available to them.
You had an excellent idea, one which great minds have already devised and implemented with success. However, for what you aim to accomplish this technique is dangerous and would most likely prove difficult to procure the necessary components.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461264 - 11/01/15 09:44 AM (8 years, 2 months ago) |
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OP you dont have the equipment to do that. and its pointless when all you need is soap and water + good SAB technique.
its not just h2o2 put through a vaporizer
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22461268 - 11/01/15 09:45 AM (8 years, 2 months ago) |
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Nobody is going to be able to use this method at home without dying so I'm not seeing the point in discussing it.
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TheMaster
PATIENCE


Registered: 07/13/13
Posts: 705
Loc: Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461312 - 11/01/15 09:52 AM (8 years, 2 months ago) |
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He had a great idea, one that many here quickly dismissed. If he had this idea thirty years ago he would be one rich sob had he patented it.
Having said that, it would probably be a better topic for the chemistry board.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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Psilosoulful

Registered: 09/05/14
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22461316 - 11/01/15 09:53 AM (8 years, 2 months ago) |
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Quote:
spacechildo said: all you need is soap and water + good SAB technique.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22461338 - 11/01/15 09:56 AM (8 years, 2 months ago) |
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Quote:
TheMaster said: He had a great idea, one that many here quickly dismissed. If he had this idea thirty years ago he would be one rich sob had he patented it.
Having said that, it would probably be a better topic for the chemistry board.
We quickly dismiss it because it's dangerous and we get good success rates without sterilizing the air in our work area. Some of us live with pets and children and people we don't want dead.
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461369 - 11/01/15 10:04 AM (8 years, 2 months ago) |
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i'm pretty sure this idea aint something new..
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TheMaster
PATIENCE


Registered: 07/13/13
Posts: 705
Loc: Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461381 - 11/01/15 10:07 AM (8 years, 2 months ago) |
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Quote:
Inocuole said: I doubt you can even get it to boiling without exponentially speeding up the rate at which it turns to water.
It was quickly dismissed because people didn't understand the chemistry behind what trees was talking about. You at least qualified your later reply by asking if this was taking place in a house.
When you have a TC saying that chemicals can't sterlize  Regardless of how impractical it is, this type of creativity should be encouraged, not criticized.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
Edited by TheMaster (11/01/15 10:20 AM)
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461384 - 11/01/15 10:08 AM (8 years, 2 months ago) |
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Quote:
Inocuole said:
Quote:
TheMaster said: He had a great idea, one that many here quickly dismissed. If he had this idea thirty years ago he would be one rich sob had he patented it.
Having said that, it would probably be a better topic for the chemistry board.
We quickly dismiss it because it's dangerous and we get good success rates without sterilizing the air in our work area. Some of us live with pets and children and people we don't want dead.
You dismiss it because your flat out stupid. Do you even know what hydrogen peroxide is? You think its dangerous - how did you figure that out? Did you know you can drink diluted concentrations of h2o2 and have no ill effect, while that same concentration you drank will kill 99.9% of all microbial life that it contacts. I'm not asking how to clean my glove box. I'm asking a question that regards chemistry.
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Edited by trees (11/01/15 10:08 AM)
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spacechildo
proletarians rise up


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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22461405 - 11/01/15 10:11 AM (8 years, 2 months ago) |
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this is mush cult. you dont have the equipment to do this. you're either trolling or just upset your idea wasnt a good one at all..
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TheMaster
PATIENCE


Registered: 07/13/13
Posts: 705
Loc: Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22461406 - 11/01/15 10:11 AM (8 years, 2 months ago) |
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-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22461417 - 11/01/15 10:14 AM (8 years, 2 months ago) |
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Quote:
spacechildo said: this is mush cult. you dont have the equipment to do this. you're either trolling or just upset your idea wasnt a good one at all..
You definitely do not know whether h2o2 will decompose after being passed over an ultra sonic humidifier bought at Walgreens.
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22461423 - 11/01/15 10:15 AM (8 years, 2 months ago) |
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I know it wont sterilize shit.
how are you gonna remove all humidity from the air first? and no, a walgreen vaporizer is not the correct equipment.
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22461437 - 11/01/15 10:19 AM (8 years, 2 months ago) |
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Quote:
spacechildo said: I know it wont sterilize shit.
how are you gonna remove all humidity from the air first? and no, a walgreen vaporizer is not the correct equipment.
Quote:
spacechildo said: I know it wont sterilize shit.
how are you gonna remove all humidity from the air first? and no, a walgreen vaporizer is not the correct equipment.
You're just another retard in the bunch. Stop trying to assert what you have no knowledge to back up.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22461440 - 11/01/15 10:19 AM (8 years, 2 months ago) |
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Quote:
trees said:
Quote:
Inocuole said:
Quote:
TheMaster said: He had a great idea, one that many here quickly dismissed. If he had this idea thirty years ago he would be one rich sob had he patented it.
Having said that, it would probably be a better topic for the chemistry board.
We quickly dismiss it because it's dangerous and we get good success rates without sterilizing the air in our work area. Some of us live with pets and children and people we don't want dead.
You dismiss it because your flat out stupid. Do you even know what hydrogen peroxide is? You think its dangerous - how did you figure that out? Did you know you can drink diluted concentrations of h2o2 and have no ill effect, while that same concentration you drank will kill 99.9% of all microbial life that it contacts. I'm not asking how to clean my glove box. I'm asking a question that regards chemistry.
Then post it somewhere applicable instead of starting shit here, you fuck.
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22461462 - 11/01/15 10:25 AM (8 years, 2 months ago) |
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Quote:
trees said:
Quote:
spacechildo said: I know it wont sterilize shit.
how are you gonna remove all humidity from the air first? and no, a walgreen vaporizer is not the correct equipment.
Quote:
spacechildo said: I know it wont sterilize shit.
how are you gonna remove all humidity from the air first? and no, a walgreen vaporizer is not the correct equipment.
You're just another retard in the bunch. Stop trying to assert what you have no knowledge to back up.
no, that's how it works. if you know this process so well why the fuck do you ask all these stupid questions then?
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spacechildo
proletarians rise up


Registered: 01/24/13
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461469 - 11/01/15 10:26 AM (8 years, 2 months ago) |
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Quote:
Inocuole said: Then post it somewhere applicable instead of starting shit here, you fuck.
he's just another noob who gets butthurt when he dont get the answers he wants. he's a dime a dozen here unfortunately..
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22461470 - 11/01/15 10:26 AM (8 years, 2 months ago) |
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Seriously, fuck off. If you're just here to insult the people who obviously have better sterile technique than you, this isn't the forum for you.
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DrCrumbs
Alpha Blue


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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461838 - 11/01/15 11:47 AM (8 years, 2 months ago) |
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What about Ozone (O3) generators? https://en.wikipedia.org/wiki/Ozone#Production
I've used this a few times, usually when I first start a new clean room for working. Then again every once in a while.
I havent used one of these SAB's yet, mostly because I havent had any serious issues. I just use a large clean empty room, that I try to keep as still as possible.
If I was just starting again I would, and I'm seriously thinking of making one (I got the materials a few weeks ago). I just havent had an issue this time around yet, I'm sure I will without a sab or flow hood.
What I'd really like to looking into is UV sterilizing. Seems like the best way to sterilize the front of the filter each time on the flow hood.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



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Re: Vaporizing H202 for glove box sterilization? [Re: DrCrumbs]
#22461892 - 11/01/15 11:55 AM (8 years, 2 months ago) |
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Most cite the danger of burns to living tissues when not used carefully. Hydrogen peroxide can be dangerous to use on the skin and even more dangerous to use internally. Most especially, be sure to dilute the stronger 35-percent food grade peroxide meticulously to avoid burning internal tissues.
That part right there is enough to deter me from attempting.
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Q&A US vs. THEM The more I learn, the less I know.
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: LocN9ne]
#22461959 - 11/01/15 12:10 PM (8 years, 2 months ago) |
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Quote:
LocN9ne said: Most cite the danger of burns to living tissues when not used carefully. Hydrogen peroxide can be dangerous to use on the skin and even more dangerous to use internally. Most especially, be sure to dilute the stronger 35-percent food grade peroxide meticulously to avoid burning internal tissues.
That part right there is enough to deter me from attempting.
It is corrosive at high concentrations but it's really not dangerous even at 30%. Ive accidentally splashed 30% on my hands a bunch of times and it turns the skin milky white and stings a bit. Definitely not deadly, and when you dilute it, it's really not a threat to anything larger than microbial cells.
I've doused tiny seedlings in diluted 30% (50 ml per gallon 30%) for sterilizing growing medium and the seedlings always survive.
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Edited by trees (11/01/15 12:11 PM)
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impatientguy
Ganjalf a very mighty lab wizard



Registered: 11/26/14
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462003 - 11/01/15 12:20 PM (8 years, 2 months ago) |
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Do it the then tell us what happens trees. Tell us what happens because none of us have tried it.
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DrCrumbs
Alpha Blue


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Re: Vaporizing H202 for glove box sterilization? [Re: DrCrumbs]
#22462054 - 11/01/15 12:30 PM (8 years, 2 months ago) |
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Back in the day when we incubated, and use h202 religiously. I would put it in the water part with a fish heater. This would cause build of higher concentrations in the incubator.
When I would inspect jars/bags the skin on my fingers would get white splotches on them from mildly being burned. It took me a while to figure out what was going on, haha.
I mean everything's dangerous, you just need to know how to properly handle things. Course unexpected things can come up.
The thing that bothers me is your adding so much moisture to your room, it seem like that would be good for your house in the long run. Course I bet this would help keep the air still. Its not like h202 solution evaporates fast like iso or something.
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PussyFart
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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22462371 - 11/01/15 01:29 PM (8 years, 2 months ago) |
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Quote:
TheMaster said: When you have a TC saying that chemicals can't sterlize 
Lol..... peroxide itself is not sterile..... prove me wrong, don't just facepalm.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462581 - 11/01/15 02:14 PM (8 years, 2 months ago) |
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Quote:
PussyFart said:
Quote:
TheMaster said: When you have a TC saying that chemicals can't sterlize 
Lol..... peroxide itself is not sterile..... prove me wrong, don't just facepalm.
Peroxide itself is just a multi oxygen molecule. Hydrogen-peroxide IS sterile.
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bodhisatta 
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462595 - 11/01/15 02:18 PM (8 years, 2 months ago) |
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Peroxide refers to usable solutions anything you can buy around town isn't sterile and won't sterilise
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PussyFart
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Re: Vaporizing H202 for glove box sterilization? [Re: bodhisatta]
#22462601 - 11/01/15 02:20 PM (8 years, 2 months ago) |
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Even if it was sterile, the second you open the bottle it isn't, so yea.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: bodhisatta]
#22462606 - 11/01/15 02:21 PM (8 years, 2 months ago) |
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Quote:
trees said:
Quote:
PussyFart said:
Quote:
TheMaster said: When you have a TC saying that chemicals can't sterlize 
Lol..... peroxide itself is not sterile..... prove me wrong, don't just facepalm.
Peroxide itself is just a multi oxygen molecule. Hydrogen-peroxide IS sterile.
Quote:
bodhisatta said: Peroxide refers to usable solutions anything you can buy around town isn't sterile and won't sterilise
I buy this locally https://www.planetnatural.com/product/h2o2-hydrogen-peroxide-29/
It's absolutely sterile
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PussyFart
Retired Cultivation Extrodinaire



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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462608 - 11/01/15 02:22 PM (8 years, 2 months ago) |
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Until u open it.....and stuff gets in it.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/01/15 02:22 PM)
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462609 - 11/01/15 02:22 PM (8 years, 2 months ago) |
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Quote:
PussyFart said: Even if it was sterile, the second you open the bottle it isn't, so yea.
Completely wrong. The outside of the bottle isn't sterile but the contents and what ever they touch is sterile.
You're definitely not knowledgeable enough about h2o2 basics to contribute to this thread.
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Edited by trees (11/01/15 02:23 PM)
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PussyFart
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462615 - 11/01/15 02:24 PM (8 years, 2 months ago) |
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Exposure to open air = not sterile.........but whatever you say....
All this is not needed with proper technique.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/01/15 02:24 PM)
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462619 - 11/01/15 02:25 PM (8 years, 2 months ago) |
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Quote:
PussyFart said: Until u open it.....and stuff gets in it.
Good for you: https://en.m.wikipedia.org/wiki/Hydrogen_peroxide
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PussyFart
Retired Cultivation Extrodinaire



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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462621 - 11/01/15 02:26 PM (8 years, 2 months ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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spacechildo
proletarians rise up


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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462662 - 11/01/15 02:39 PM (8 years, 2 months ago) |
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wikipedia 
no need for tags or even this forum anymore, wiki to the rescue
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TheMaster
PATIENCE


Registered: 07/13/13
Posts: 705
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462749 - 11/01/15 02:57 PM (8 years, 2 months ago) |
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Quote:
PussyFart said:
Quote:
TheMaster said: When you have a TC saying that chemicals can't sterlize 
Lol..... peroxide itself is not sterile..... prove me wrong, don't just facepalm.
Quote:
TheMaster said:
Quote:
PussyFart said:
Quote:
trees said: I don't know how stable H202 is when vaporized by a humidifier, maybe a chemist who knows can chime in?
I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
I know that H202 is not very stable and might decompose in the ultra sonic vaporizer?
Chemicals sanitize, heat and filtration sterilize.
There is no need for peroxide in this hobby outside of treating cobweb mold.
At the proper concentrations and with the appropriate engineering, hydrogen peroxide is indeed a sterilant. http://www.cdc.gov/hicpac/Disinfection_Sterilization/13_02sterilization.html Microbicidal Activity. This process has the ability to inactivate a broad range of microorganisms, including resistant bacterial spores. Studies have been conducted against vegetative bacteria (including mycobacteria), yeasts, fungi, viruses, and bacterial spores469, 721, 856, 881-883, 890-893. Like all sterilization processes, the effectiveness can be altered by lumen length, lumen diameter, inorganic salts, and organic materials469, 721, 855, 856, 890, 891, 893.
Trees, you're probably not going to be able to accomplish your goal with over the counter hydrogen peroxide. At that low of concentration the sporicidal activity will be greatly dimished. Peracetic acid in combination with low concentration hydrogen peroxide would boost efficacy as a sterilant. Also, this technique also requires you initiate free radical generation of the hydrogen peroxide. It seems a bit out of reach for what most people have available to them.
You had an excellent idea, one which great minds have already devised and implemented with success. However, for what you aim to accomplish this technique is dangerous and would most likely prove difficult to procure the necessary components.
I guess you missed the part where I already proved you wrong. Chemicals such as hydrogen peroxide are used everyday as a sterlilant to prevent transfer of deadly pathogens. Its efficacy has been proven in multiple peer reviewed studies.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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PussyFart
Retired Cultivation Extrodinaire



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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462756 - 11/01/15 02:59 PM (8 years, 2 months ago) |
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Quote:
PussyFart said:

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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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impatientguy
Ganjalf a very mighty lab wizard



Registered: 11/26/14
Posts: 5,054
Loc: USA
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22462757 - 11/01/15 02:59 PM (8 years, 2 months ago) |
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I like to use hydrogen peroxide in my dab rig to keep my reclaim from trying to grow mold. Lol
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TheMaster
PATIENCE


Registered: 07/13/13
Posts: 705
Loc: Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462796 - 11/01/15 03:11 PM (8 years, 2 months ago) |
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It's OK to be wrong, dude. That is how people learn.
When someone points out that I am misinformed I typically say something like, "wow, yeah you were right, thanks for reducing my ignorance".
At the end of the day, I'm going to trust the CDC's guidelines when it comes to necessary definitions of sterility, not yours.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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spacechildo
proletarians rise up


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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22462802 - 11/01/15 03:13 PM (8 years, 2 months ago) |
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you clearly dont understand what the cdc page said. explain to me how h2o2 and lumens are connected... 
Quote:
TheMaster said: When someone points out that I am misinformed I typically say something like, "wow, yeah you were right, thanks for reducing my ignorance".
good! now thank pussyfart and move along
Edited by spacechildo (11/01/15 03:15 PM)
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TheMaster
PATIENCE


Registered: 07/13/13
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Loc: Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22462838 - 11/01/15 03:22 PM (8 years, 2 months ago) |
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I guess you didn't take the time to read the link I posted. Here is another little piece. Feel free to read more if you'd like to learn more about the process. In this particular usage of hydrogen peroxide as a sterilant, free radicals are formed via radiowaves. I'm not trying to get in a pissing match here. I happen to have a bit of experience with these types of systems. This is but one of many methods that use hydrogen peroxide, alone or in combination with other chemical species, to sterlize surfaces.
In the late 1980s the first hydrogen peroxide gas plasma system for sterilization of medical and surgical devices was field-tested. According to the manufacturer, the sterilization chamber is evacuated and hydrogen peroxide solution is injected from a cassette and is vaporized in the sterilization chamber to a concentration of 6 mg/l. The hydrogen peroxide vapor diffuses through the chamber (50 minutes), exposes all surfaces of the load to the sterilant, and initiates the inactivation of microorganisms. An electrical field created by a radio frequency is applied to the chamber to create a gas plasma. Microbicidal free radicals (e.g., hydroxyl and hydroperoxyl) are generated in the plasma. The excess gas is removed and in the final stage (i.e., vent) of the process the sterilization chamber is returned to atmospheric pressure by introduction of high-efficiency filtered air. The by-products of the cycle (e.g., water vapor, oxygen) are nontoxic and eliminate the need for aeration.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
Edited by TheMaster (11/01/15 07:19 PM)
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spacechildo
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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22462857 - 11/01/15 03:28 PM (8 years, 2 months ago) |
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I swear this happens every time pubbers venture into mush cult...
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TheMaster
PATIENCE


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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22462885 - 11/01/15 03:37 PM (8 years, 2 months ago) |
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Not a pubber, more of a lurker really.
I just don't like seeing people acting like they know everything about a topic some have devoted their lives to. When it comes to stuff like this, I know my shit. The info is out there if you take the time to research it.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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spacechildo
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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22462896 - 11/01/15 03:42 PM (8 years, 2 months ago) |
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and we're here trying to teach people how to grow, it gets kinda annoying when we have to clean up after other peoples bad advice constantly.
devoted your life to h2o2...good for you!
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cronicr



Registered: 08/07/11
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22462910 - 11/01/15 03:46 PM (8 years, 2 months ago) |
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The answer is simple, you cant sterilize a sab and keep it sterile keep it simple and get it wet,sabs are great because they are cheap and effective.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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TheMaster
PATIENCE


Registered: 07/13/13
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22462943 - 11/01/15 03:54 PM (8 years, 2 months ago) |
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Lol. I agree. It's too bad really; so many threads with the same dated info are out there. This wasn't the best place for this type of thread. That's part of why I recommended the OP repost in the chemistry board where it would be better received.
I don't think there is any need for hostility though. It's nice if everyone can maintain some level of civility and not takes things too personally.
I've devoted my life to engineering and science. The first step if you don't know something is to do research and make as few assumptions as possible in the process.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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cronicr



Registered: 08/07/11
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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22462960 - 11/01/15 03:58 PM (8 years, 2 months ago) |
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There is just no feasable way to do what the op wants to, better off just attaching a mister to the bottle and going traditional.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: PussyFart]
#22463525 - 11/01/15 06:14 PM (8 years, 2 months ago) |
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Quote:
PussyFart said:
Quote:
PussyFart said: I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
Chemicals sanitize, heat sterilizes.
There is no need for peroxide in this hobby outside of treating cobweb mold.
Also I've seen a lot of your grows, and you often lose plenty of your hard work to contamination.
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cronicr



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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: trees]
#22463532 - 11/01/15 06:15 PM (8 years, 2 months ago) |
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You here to argue or discuss shit?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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impatientguy
Ganjalf a very mighty lab wizard



Registered: 11/26/14
Posts: 5,054
Loc: USA
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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: trees]
#22463713 - 11/01/15 06:47 PM (8 years, 2 months ago) |
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Quote:
trees said:
Quote:
PussyFart said:
Quote:
PussyFart said: I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
Chemicals sanitize, heat sterilizes.
There is no need for peroxide in this hobby outside of treating cobweb mold.
Also I've seen a lot of your grows, and you often lose plenty of your hard work to contamination.
Lol at this point it's just to be a dick.
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cronicr



Registered: 08/07/11
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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: impatientguy]
#22463723 - 11/01/15 06:48 PM (8 years, 2 months ago) |
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Getting to that point myself
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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spacechildo
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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: impatientguy]
#22463780 - 11/01/15 06:59 PM (8 years, 2 months ago) |
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lol, how it goes again, if you cant post without showing..respect...hmm..nah cant remember
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PussyFart
Retired Cultivation Extrodinaire



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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: trees]
#22463918 - 11/01/15 07:31 PM (8 years, 2 months ago) |
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Quote:
trees said: Also I've seen a lot of your grows, and you often lose plenty of your hard work to contamination.
Yea, shit happens......
This doesn't mean anything tho.

--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/01/15 07:31 PM)
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impatientguy
Ganjalf a very mighty lab wizard



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Re: Vaporizing H202 for container, chamber, glove box sterilization? [Re: cronicr]
#22464216 - 11/01/15 08:30 PM (8 years, 2 months ago) |
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So was I.
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nitrodigital
MycoAddicted


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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22662369 - 12/16/15 09:37 AM (8 years, 1 month ago) |
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There is a ton of disinfo on the topics of peroxide and O3 on shroomery from people with a lot of mushroom growing experience . I've heard this stuff will "kill you". Hehe, ... so will a car, so will bleach, so will lysol if you drink it!
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PussyFart
Retired Cultivation Extrodinaire



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Re: Vaporizing H202 for glove box sterilization? [Re: nitrodigital]
#22662394 - 12/16/15 09:45 AM (8 years, 1 month ago) |
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It's all about the context......
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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nitrodigital
MycoAddicted


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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22662967 - 12/16/15 12:31 PM (8 years, 1 month ago) |
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you bet,... its all poison unless used in the correct concentration, and used responsibly. I agree it can kill you if not used correctly but these things have many uses if used responsibly.
Edited by nitrodigital (12/18/15 02:25 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Vaporizing H202 for glove box sterilization? [Re: nitrodigital]
#22664294 - 12/16/15 05:57 PM (8 years, 1 month ago) |
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i almost remember this thread, i love drugs
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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