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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22461470 - 11/01/15 10:26 AM (8 years, 2 months ago) |
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Seriously, fuck off. If you're just here to insult the people who obviously have better sterile technique than you, this isn't the forum for you.
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DrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
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Re: Vaporizing H202 for glove box sterilization? [Re: Inocuole]
#22461838 - 11/01/15 11:47 AM (8 years, 2 months ago) |
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What about Ozone (O3) generators? https://en.wikipedia.org/wiki/Ozone#Production
I've used this a few times, usually when I first start a new clean room for working. Then again every once in a while.
I havent used one of these SAB's yet, mostly because I havent had any serious issues. I just use a large clean empty room, that I try to keep as still as possible.
If I was just starting again I would, and I'm seriously thinking of making one (I got the materials a few weeks ago). I just havent had an issue this time around yet, I'm sure I will without a sab or flow hood.
What I'd really like to looking into is UV sterilizing. Seems like the best way to sterilize the front of the filter each time on the flow hood.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Vaporizing H202 for glove box sterilization? [Re: DrCrumbs]
#22461892 - 11/01/15 11:55 AM (8 years, 2 months ago) |
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Most cite the danger of burns to living tissues when not used carefully. Hydrogen peroxide can be dangerous to use on the skin and even more dangerous to use internally. Most especially, be sure to dilute the stronger 35-percent food grade peroxide meticulously to avoid burning internal tissues.
That part right there is enough to deter me from attempting.
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Q&A US vs. THEM The more I learn, the less I know.
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: LocN9ne]
#22461959 - 11/01/15 12:10 PM (8 years, 2 months ago) |
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Quote:
LocN9ne said: Most cite the danger of burns to living tissues when not used carefully. Hydrogen peroxide can be dangerous to use on the skin and even more dangerous to use internally. Most especially, be sure to dilute the stronger 35-percent food grade peroxide meticulously to avoid burning internal tissues.
That part right there is enough to deter me from attempting.
It is corrosive at high concentrations but it's really not dangerous even at 30%. Ive accidentally splashed 30% on my hands a bunch of times and it turns the skin milky white and stings a bit. Definitely not deadly, and when you dilute it, it's really not a threat to anything larger than microbial cells.
I've doused tiny seedlings in diluted 30% (50 ml per gallon 30%) for sterilizing growing medium and the seedlings always survive.
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Edited by trees (11/01/15 12:11 PM)
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impatientguy
Ganjalf a very mighty lab wizard



Registered: 11/26/14
Posts: 5,054
Loc: USA
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462003 - 11/01/15 12:20 PM (8 years, 2 months ago) |
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Do it the then tell us what happens trees. Tell us what happens because none of us have tried it.
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DrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
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Re: Vaporizing H202 for glove box sterilization? [Re: DrCrumbs]
#22462054 - 11/01/15 12:30 PM (8 years, 2 months ago) |
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Back in the day when we incubated, and use h202 religiously. I would put it in the water part with a fish heater. This would cause build of higher concentrations in the incubator.
When I would inspect jars/bags the skin on my fingers would get white splotches on them from mildly being burned. It took me a while to figure out what was going on, haha.
I mean everything's dangerous, you just need to know how to properly handle things. Course unexpected things can come up.
The thing that bothers me is your adding so much moisture to your room, it seem like that would be good for your house in the long run. Course I bet this would help keep the air still. Its not like h202 solution evaporates fast like iso or something.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Re: Vaporizing H202 for glove box sterilization? [Re: TheMaster]
#22462371 - 11/01/15 01:29 PM (8 years, 2 months ago) |
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Quote:
TheMaster said: When you have a TC saying that chemicals can't sterlize 
Lol..... peroxide itself is not sterile..... prove me wrong, don't just facepalm.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462581 - 11/01/15 02:14 PM (8 years, 2 months ago) |
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Quote:
PussyFart said:
Quote:
TheMaster said: When you have a TC saying that chemicals can't sterlize 
Lol..... peroxide itself is not sterile..... prove me wrong, don't just facepalm.
Peroxide itself is just a multi oxygen molecule. Hydrogen-peroxide IS sterile.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462595 - 11/01/15 02:18 PM (8 years, 2 months ago) |
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Peroxide refers to usable solutions anything you can buy around town isn't sterile and won't sterilise
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: bodhisatta]
#22462601 - 11/01/15 02:20 PM (8 years, 2 months ago) |
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Even if it was sterile, the second you open the bottle it isn't, so yea.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: bodhisatta]
#22462606 - 11/01/15 02:21 PM (8 years, 2 months ago) |
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Quote:
trees said:
Quote:
PussyFart said:
Quote:
TheMaster said: When you have a TC saying that chemicals can't sterlize 
Lol..... peroxide itself is not sterile..... prove me wrong, don't just facepalm.
Peroxide itself is just a multi oxygen molecule. Hydrogen-peroxide IS sterile.
Quote:
bodhisatta said: Peroxide refers to usable solutions anything you can buy around town isn't sterile and won't sterilise
I buy this locally https://www.planetnatural.com/product/h2o2-hydrogen-peroxide-29/
It's absolutely sterile
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462608 - 11/01/15 02:22 PM (8 years, 2 months ago) |
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Until u open it.....and stuff gets in it.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/01/15 02:22 PM)
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462609 - 11/01/15 02:22 PM (8 years, 2 months ago) |
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Quote:
PussyFart said: Even if it was sterile, the second you open the bottle it isn't, so yea.
Completely wrong. The outside of the bottle isn't sterile but the contents and what ever they touch is sterile.
You're definitely not knowledgeable enough about h2o2 basics to contribute to this thread.
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Edited by trees (11/01/15 02:23 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462615 - 11/01/15 02:24 PM (8 years, 2 months ago) |
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Exposure to open air = not sterile.........but whatever you say....
All this is not needed with proper technique.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/01/15 02:24 PM)
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trees



Registered: 02/08/09
Posts: 9,194
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462619 - 11/01/15 02:25 PM (8 years, 2 months ago) |
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Quote:
PussyFart said: Until u open it.....and stuff gets in it.
Good for you: https://en.m.wikipedia.org/wiki/Hydrogen_peroxide
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Re: Vaporizing H202 for glove box sterilization? [Re: trees]
#22462621 - 11/01/15 02:26 PM (8 years, 2 months ago) |
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462662 - 11/01/15 02:39 PM (8 years, 2 months ago) |
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wikipedia 
no need for tags or even this forum anymore, wiki to the rescue
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TheMaster
PATIENCE


Registered: 07/13/13
Posts: 705
Loc: Earth
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462749 - 11/01/15 02:57 PM (8 years, 2 months ago) |
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Quote:
PussyFart said:
Quote:
TheMaster said: When you have a TC saying that chemicals can't sterlize 
Lol..... peroxide itself is not sterile..... prove me wrong, don't just facepalm.
Quote:
TheMaster said:
Quote:
PussyFart said:
Quote:
trees said: I don't know how stable H202 is when vaporized by a humidifier, maybe a chemist who knows can chime in?
I thought it would be a good idea to fill a humidifier with hydrogen peroxide solution and pipe the vapor into the container, glove box, or fruiting chamber effectively sterilizing every nook and cranny.
I know that H202 is not very stable and might decompose in the ultra sonic vaporizer?
Chemicals sanitize, heat and filtration sterilize.
There is no need for peroxide in this hobby outside of treating cobweb mold.
At the proper concentrations and with the appropriate engineering, hydrogen peroxide is indeed a sterilant. http://www.cdc.gov/hicpac/Disinfection_Sterilization/13_02sterilization.html Microbicidal Activity. This process has the ability to inactivate a broad range of microorganisms, including resistant bacterial spores. Studies have been conducted against vegetative bacteria (including mycobacteria), yeasts, fungi, viruses, and bacterial spores469, 721, 856, 881-883, 890-893. Like all sterilization processes, the effectiveness can be altered by lumen length, lumen diameter, inorganic salts, and organic materials469, 721, 855, 856, 890, 891, 893.
Trees, you're probably not going to be able to accomplish your goal with over the counter hydrogen peroxide. At that low of concentration the sporicidal activity will be greatly dimished. Peracetic acid in combination with low concentration hydrogen peroxide would boost efficacy as a sterilant. Also, this technique also requires you initiate free radical generation of the hydrogen peroxide. It seems a bit out of reach for what most people have available to them.
You had an excellent idea, one which great minds have already devised and implemented with success. However, for what you aim to accomplish this technique is dangerous and would most likely prove difficult to procure the necessary components.
I guess you missed the part where I already proved you wrong. Chemicals such as hydrogen peroxide are used everyday as a sterlilant to prevent transfer of deadly pathogens. Its efficacy has been proven in multiple peer reviewed studies.
-------------------- "Live as if you were to die tomorrow. Learn as if you were to live forever." Mahatma Gandhi
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 17 hours
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Re: Vaporizing H202 for glove box sterilization? [Re: PussyFart]
#22462756 - 11/01/15 02:59 PM (8 years, 2 months ago) |
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Quote:
PussyFart said:

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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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impatientguy
Ganjalf a very mighty lab wizard



Registered: 11/26/14
Posts: 5,054
Loc: USA
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Re: Vaporizing H202 for glove box sterilization? [Re: spacechildo]
#22462757 - 11/01/15 02:59 PM (8 years, 2 months ago) |
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I like to use hydrogen peroxide in my dab rig to keep my reclaim from trying to grow mold. Lol
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