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OfflineTemporary Death
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Registered: 10/05/15
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Last seen: 8 years, 1 month
APE Germinated in Syringe, or...?
    #22458703 - 10/31/15 05:55 PM (8 years, 2 months ago)

This is my first post, I have been a very long time Shroomery follower and only recently gotten into cultivation this year.
I am curious about an order that I placed through a spore sponsor,  i've been very happy with all of my previous purchases and this purchase included, but one of these 5 syringes (albino penis envy) I'm curious if this is for sure germinated spores within the solution, and/or if this breed is known to have white spores that I'm seeing?
If this is growing mycelium (created 9/15) when would it be recommended to use this syringe by at the latest?
Thanks everyone! I think this should be in the correct section.





And also I was hoping to save my first post to share my experience of reach complete death with the help from our fungal friends here, that will have to be saved for later tho! Thanks!


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Invisiblemicro
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Re: APE Germinated in Syringe, or...? [Re: Temporary Death]
    #22459778 - 10/31/15 10:50 PM (8 years, 2 months ago)

http://sporeworks.com/Psilocybe-cubensis-Albino-Penis-Envy-APE-ver.-1.0-Spore-Syringe-Microscopy-Kit.html

Quote:

Spores: Few, transparent, subellipsoid on 4-spored basidia, white in deposit.





That said, no clue if they have germinated. The liquid looks yellow; do you know if that is a nutrient broth or water/saline? It should take a while for them to germinate if they are kept refrigerated but it has been known to happen. If it is water or saline you should keep it cold and use it pretty soon, since the mycelium will not survive an extended period of time without food.

One thing you can do is flick the syringe several times and see if it breaks up the clump. Spores will clump together, but in a hyphal network the cells are actually connected. So, if it's really difficult to break up it is likely mycelium.


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OfflineTemporary Death
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Registered: 10/05/15
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Last seen: 8 years, 1 month
Re: APE Germinated in Syringe, or...? [Re: micro]
    #22464505 - 11/01/15 09:38 PM (8 years, 2 months ago)

I'm not familiar with a spore vendor sending a spore syringe of a cubensis in nutrient water. But I've just inoculated a quart of rye with it tonight so I suppose the time it takes to see myc will offer the answer of it being pre germinated or not right?

APE are clear spores too, so I wonder about this yellow liquid in the syringe.

Thanks


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OfflineInextinctus
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Registered: 06/17/15
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Re: APE Germinated in Syringe, or...? [Re: Temporary Death]
    #22481105 - 11/05/15 01:02 PM (8 years, 2 months ago)

looks like a fat ass spore clump to me, do what micro said, swish and flick the shit out of it and if it breaks up you gotta spore clumpy :laugh:


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OfflineInextinctus
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Registered: 06/17/15
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Re: APE Germinated in Syringe, or...? [Re: Inextinctus]
    #22481112 - 11/05/15 01:04 PM (8 years, 2 months ago)

Quote:

And also I was hoping to save my first post to share my experience of reach complete death with the help from our fungal friends here, that will have to be saved for later tho! Thanks!




save it for your 42nd, 69th or 420th posts :kimjong:


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Soundcloud.com/inextinctus
what are we but a different combination of the elements we consume?
"So then I :lsd:"



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Offlinedharmadeans
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Re: APE Germinated in Syringe, or...? [Re: Temporary Death]
    #22581522 - 11/27/15 09:46 AM (8 years, 2 months ago)

Serendipitously found this post....same thing just happened to me from same sponsor I'm sure. Makes me think we are in the clear...love to hear if yours was clean vs contam...


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OfflineTemporary Death
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Registered: 10/05/15
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Last seen: 8 years, 1 month
Re: APE Germinated in Syringe, or...? [Re: dharmadeans]
    #22605169 - 12/02/15 05:18 PM (8 years, 1 month ago)

It definetly wasn't contaminated, could've been a blob of the clear spores as they apparently come, or some did germinate.

The liquid culture I made thrived way quicker than 3 others I started (golden teacher, malabar(looks like contam in this jar, assume from syringe since I'm finatic about sterility), pf classic (which still are going sooo slow)

I inoculated 6 rye berry quarts of PE and 6 quarts of this APE LC same time, PE is more than done, although the mycelium doesn't look quite as "sarurated" in white as past projects, but the APE had the thickest whitest myc starting comparatively to the PE, except the APE has halted, at like barley 4% colonized but did shake it a bit early experimenting and wanting to spread around what I thought would disperse the "at the time" thriving healthy APE myc

Shit, owell, first PE tub going tonight or tomorrow, will have to research why some rye grain quarts stall out later tonight, unless someone has a quick answer here!

Peace!


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OfflineTemporary Death
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Registered: 10/05/15
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Last seen: 8 years, 1 month
Re: APE Germinated in Syringe, or...? [Re: Temporary Death]
    #22638268 - 12/10/15 12:18 PM (8 years, 1 month ago)

My rye berrie quarts (all 6 of them) look apparently contaminated from this APE syringe that Inocculated a liquid culture jar (along with  after very slow growth while 4 other strains finished colonizing in 11 days,
The ape LC grew faster than the rest, looked good at first, then 25 days later halted midway and now has this slimy pale yellow gray substance around the berries.

Assuming the rest of my 4 other strains inocc'd to LC did just fine under all same sterile (SAB) tequnique, was this APE syringe contaminated to begin hence the question ability of the original post on this thread?

I'll inocc a half pint straight from the syringe to test, still procrastinating agar method purchases.


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OfflineTemporary Death
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Registered: 10/05/15
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Re: APE Germinated in Syringe, or...? [Re: Temporary Death]
    #22642011 - 12/11/15 09:48 AM (8 years, 1 month ago)

Bumbity bumble bee,

Anyone opinions? Or shall a new tgread be re mreated.
The APE was going strong in the quart jars first 4 days, shook them, then just halted now all going grey ceramic slimed stuff on the rye berries.


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OfflinePeter Dinklage
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Registered: 05/23/15
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Re: APE Germinated in Syringe, or...? [Re: Temporary Death]
    #22665477 - 12/16/15 10:28 PM (8 years, 1 month ago)

Just had the same thing happen to me. Same vendor and APE strain. It looks like LC. I put a few drops on some agar dishes. Have you had any luck with yours?  My first thought was I received a mislabeled syringe of an edible LC.


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OfflineKnoxstein
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Registered: 12/28/15
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Re: APE Germinated in Syringe, or...? [Re: Peter Dinklage]
    #22734457 - 01/04/16 02:46 AM (8 years, 27 days ago)

same thing with me also. my syringe looked like they had germed inside. mine had a orange color, and the spores looked very milky. lets just say they not doing their thing.


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OfflinePeter Dinklage
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Re: APE Germinated in Syringe, or...? [Re: Knoxstein]
    #22736411 - 01/04/16 04:36 PM (8 years, 27 days ago)

i squirted some on agar and I all I got was what appeared to be yeast.


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OfflineKnoxstein
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Re: APE Germinated in Syringe, or...? [Re: Peter Dinklage]
    #22736949 - 01/04/16 07:39 PM (8 years, 27 days ago)

same. I got nothing, maybe the new APES we all got are all sterile.


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InvisibleJake McBaked
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Re: APE Germinated in Syringe, or...? [Re: Knoxstein]
    #22737785 - 01/04/16 11:06 PM (8 years, 26 days ago)

you guys should contact the vendor and if it isnt resolved make a thread in the sponsor forums


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OfflineKnoxstein
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Re: APE Germinated in Syringe, or...? [Re: Jake McBaked]
    #22740995 - 01/05/16 06:11 PM (8 years, 26 days ago)

i would contact the vendor, but since the vendor sells the spores for mycology, and not for cultivation I don't know if they will get back with me if I tell them the spores did not germ. I'm not too upset about it though I have been pleased with all my previous purchases from the vendor.


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Offlinedharmadeans
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Registered: 02/24/11
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Re: APE Germinated in Syringe, or...? [Re: Knoxstein]
    #23037496 - 03/23/16 12:49 PM (7 years, 10 months ago)

Well, put mine to grain, came out perfect,...ugliest syringe I've seen and would've thought it to be contam but it's not!


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