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OfflineRo0k
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Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
MS Syringe to Agar on 10/19. Should I be seeing something by now?
    #22457199 - 10/31/15 10:43 AM (8 years, 2 months ago)

Made the Amazonian spore syringe myself:

Shot-glass upside-down in a 1/2 pint jar and another half-pint with distilled water.  PC'd at 15psi for an hour or so.  Left overnight to cool.  Put the whole works in my SAB, which had been misted with soapy water and left overnight.  Upended the shotty, scraped a good 1/3 of the spores off the print (from a trusted supplier) into the glass with a flame sterilized knife (did let it cool some, but could have been too hot and killed the spores?), filled with the sterilized distilled water.  Brand-new sterile syringe from the wrapper, brand-new needle which I flamed just to be sure.  Quenched the hot needle in the sterilzed half-pint water then aspirated the syringe 3-4 times.  Made 2 syringes this way. 

36 hours later, I shook the syringe, flamed the needle and put 2 drops onto a PDA agar plate on 10/19.  I wrapped with parafilm and left it to colonize at ~75.  Checked today 12 days later and I'm seeing NOTHING.  No bacteria, no mould, no mycelium, nothing. 



So the good news is my sterile technique looks good.  The bad news is I'm not seeing any growth.  Shouldn't I be seeing something by now?  I keep reading stories about how guys get spores popping in 4-6 days and I"m almost a 2 weeks with zero visible growth.  Did I do something wrong? 

Yeah yeah, I know now that spore --> syringe --> plate is not the best way.  I did another last night with an innoc loop.


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InvisibleStickyIcky Fingers
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Registered: 12/03/13
Posts: 178
Re: MS Syringe to Agar on 10/19. Should I be seeing something by now? [Re: Ro0k]
    #22457289 - 10/31/15 11:08 AM (8 years, 2 months ago)

The last plate I started from a print took  3 weeks and I have had multiple ones take that long. No growth is a good sign contams usually grow faster than myc give it some more time and see what happens. I rarely have the rates of growth that are posted on here and my temp is often around 65 degrees F.



Edited by StickyIcky Fingers (10/31/15 11:11 AM)


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