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Ifishhigh
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Registered: 03/21/15
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Someone mind helping a newb
#22456653 - 10/31/15 07:04 AM (8 years, 2 months ago) |
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I'm doing the pf tek for beginners. I just have a statement and a question . First will be my statement. I like to thank everyone for answering all my questions up to this point I greatly appreciate it. They must have been decent questions because I never got flamed. Ok so on with it.......
I was preparing my jars for sterilization. I'm doing 5 right now than the other 5 tonight. My pot can't fit more than 5 at a time. The thing is I followed the 2 1 1 ratio and I made sure not to pack the jars I filled them loosely but I came up short by about half a jar. Is this common ? I just made a new batch again 2 1 1 to fill the jar completely and tossed the rest. I know it's wasteful but the verm and flour are cheap and easy to get in my area . I'm using 1/2 pint wide mouth. The only reason I ask if it's common to be short is because I wonder if I messed up something in the mixing or filling.
Also on a quick note, I have a tight schedule. I'm going to pc sterilize the jars today. I can't inoculate until next weekend. Is this a problem ? How should I keep the jars until the next step. Keep the foil on and put them in a shoe box until ready was my plan. Any suggestions would be great. Will I increase contamination possibilities by waiting a week ?
Thanks and happy Halloween
P.s lets go mets
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Psilosoulful

Registered: 09/05/14
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Re: Someone mind helping a newb [Re: Ifishhigh]
#22456689 - 10/31/15 07:25 AM (8 years, 2 months ago) |
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Did you provide a half an inch dry verm layer at the top of each jar to serve as the contamination barrier? This could be why you came up short on your last jar.
Once your jars are properly sterilized, just take the foil off and leave them on a shelf until you get back, they'll be fine!
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Machiavelliavore
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Re: Someone mind helping a newb [Re: Ifishhigh]
#22456709 - 10/31/15 07:40 AM (8 years, 2 months ago) |
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Just store them in a cool place and leave the foil on. The volume of substrate for a given recipe can probably vary a bit depending on what type of vermiculite you use, and what the geometry is, perhaps based on the flour grind, and how it gets packed together.
I've found that it seems to add up fairly linearly with fine verm. 2/3 cup verm, 1/3 cup water, 1/3 cup flour = 1+ cup of sub
I would guess you use like 2/3's to 3/4 of a cup of substrate per jar if you used a verm barrier rather than injection ports. So, 1/2 cup verm, 1/4 cup water, 1/4 cup flour per jar or so. Doesn't matter.
In the future I would try to prep before you use them. Never great to have sub sitting around, especially if it's only steam sterilized. Should be fine though.
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Ifishhigh
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Thanks for the replies. I did use fine verm with a dry verm barrier. It wasn't too off i was only a half a jar short. I also covered holes with micro pore tape. The reason I have to leave them for a week is Halloween today mets game tonight and huge nfl fan for tomorrow. Than during week days my schedule is crazy. The leaving foil on or off in the meantime is debatable I assume since I got 2 different answeres.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



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Re: Someone mind helping a newb [Re: Ifishhigh]
#22456824 - 10/31/15 08:26 AM (8 years, 2 months ago) |
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You don't need the foil or the tape since you have the dry verm layer.
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Q&A US vs. THEM The more I learn, the less I know.
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Psilosoulful

Registered: 09/05/14
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Re: Someone mind helping a newb [Re: Ifishhigh]
#22456825 - 10/31/15 08:26 AM (8 years, 2 months ago) |
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I think Mach meant to say to leave the foil off. The reason is, after PC'ing your jars, there will be a lot of trapped condensation beneath the foil. That excessive moisture will create a microclimate for bacteria to form on your jar lid and eventually work it's way into your jars through the holes (even if you covered them with tape). Micropore is not water resistant. This will bring contams closer to your sterilized brf mix and the dry verm layer will be compromised as a result of the excess moisture.
So, this is why it's best to leave the foil off for long term storage, as you want that moisture on the jar lid to evaporate as quickly as possible.
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Ifishhigh
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Quote:
Psilosoulful said: I think Mach meant to say to leave the foil off. The reason is, after PC'ing your jars, there will be a lot of trapped condensation beneath the foil. That excessive moisture will create a microclimate for bacteria to form on your jar lid and eventually work it's way into your jars through the holes (even if you covered them with tape). Micropore is not water resistant. This will bring contams closer to your sterilized brf mix and the dry verm layer will be compromised as a result of the excess moisture.
So, this is why it's best to leave the foil off for long term storage, as you want that moisture on the jar lid to evaporate as quickly as possible. 
This sounds good. So I'll take the foil off. I'll leave the tape on for the time being. Ill take the tape off after I inoculate or just before ? The video I'm following never shows or says when to take it off. It does seem when he puts them on the shelf to colonize that the tape isint on the lids anymore
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Re: Someone mind helping a newb [Re: Ifishhigh]
#22456916 - 10/31/15 09:11 AM (8 years, 2 months ago) |
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When i make jars, i always come up short on one jar, so i have to make another batch. its pretty common in my experience.
I usually mix the old batch with the new one using seperate bowls. But if u threw away the little bit of old batch, its no big deal.
As for waiting a week. I will say, there is some risk for infection. Its always best to shoot up jars *right* after they have completely cooled from PCing or steam-sterilized, which is about 12-16 Hours of cool time.
But if you keep the jars in a protected, draft-free area with the tape on, you should be fine. Just make sure to wipe the outside of the jars with some alcohol, including the lid, before you shoot them up with spores.
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Ifishhigh
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Registered: 03/21/15
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I thought I read on these boards that it's ok to sterilize ahead of time with no worries. I'll stay up all night if I have too but sure didn't want too
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Psilosoulful

Registered: 09/05/14
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Might as well keep the tape on now since it'll be sitting on a shelf and will most likely collect dust on the top of the jar lid. Extra protection is always good.
When you're ready to inoculate (making sure you do all your work in a SAB), you can wipe down the lids with iso alcohol to sanitize it, flame sterilize your needle, then inject through the tape.
Use 1/4 cc per hole and don't recover them with tape afterwards, that's what the dry verm layer is there for . It'll supply much needed GE for your colonizing cakes and is the primary defense against contams, as long as it stays dry!
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Psilosoulful

Registered: 09/05/14
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Re: Someone mind helping a newb [Re: Ifishhigh]
#22457044 - 10/31/15 10:04 AM (8 years, 2 months ago) |
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Quote:
Ifishhigh said: I thought I read on these boards that it's ok to sterilize ahead of time with no worries. I'll stay up all night if I have too but sure didn't want too
The jars will stay good for weeks after proper sterilization, just make sure you take the foil off before storing.
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