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Invisiblerams
MushTafa
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Registered: 07/16/15
Posts: 423
Loc: Mt. Everest Base camp
something settled in the Media Slants after sterilization
    #22456346 - 10/31/15 01:46 AM (8 years, 2 months ago)

Attempted Agar for the first time today.
Made PDA with the suggested tek (200ml broth from 40gm potato, filtered with a
clean cloth, reboiled and added 4gm of Dextrose and 4gm of Agar) and poured my test tubes and few "quarter whiskey flat bottles". However after sterilizing them in the PC I noticed the contents settling at the bottom and forming a ball kinda structure in water in every TT and bottle. I had to give them a shake to disintegrate the contents evenly but tiny particles still remained visible (especially in bottles). 
Wanna know why this happened? is it still OK?

PS. Have already inoculated the slants and bottles hence no images.


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InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
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Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: something settled in the Media Slants after sterilization [Re: rams]
    #22456362 - 10/31/15 02:08 AM (8 years, 2 months ago)

I sure do... and it'll be fine...I always have some sediment when I'm fuckin with potatoes


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