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Runegaldr
Stranger
Registered: 10/30/15
Posts: 4
Last seen: 8 years, 2 months
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Chemical analysis of Psilocybe sp. *DELETED*
#22452548 - 10/30/15 07:28 AM (8 years, 3 months ago) |
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Post deleted by RunegaldrReason for deletion: Seeing as people shrug off a stranger, I see no reason to benefit this community with my research.
Edited by Runegaldr (11/09/15 06:52 AM)
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Mescalitoe
Psychonaut



Registered: 05/14/14
Posts: 584
Loc: California!
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Re: Chemical analysis of Psilocybe sp. [Re: Runegaldr]
#22452588 - 10/30/15 07:43 AM (8 years, 3 months ago) |
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I'm sure you know you can buy prints off the internet.
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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Re: Chemical analysis of Psilocybe sp. [Re: Mescalitoe]
#22452800 - 10/30/15 09:15 AM (8 years, 3 months ago) |
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i'm not sure what kind of laws estonia has, i know a lot of eastern blok countries are down right draconian with regard to importing mushroom cultures, much less active spores. My advice would be to start in Amsterdam where they recently started grow 'stones' or truffles. They have several varieties for sale and perhaps you could see if there is a difference between them on a chemical level.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Chemical analysis of Psilocybe sp. [Re: Runegaldr]
#22453372 - 10/30/15 12:10 PM (8 years, 3 months ago) |
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Quote:
Runegaldr said: My work is to compare different growing methods and species(and varieties) for their alkaloid content and hopefully even some bioactivity testing on various cell cultures.
As far as species, Psilocybe azurescens and Psilocybe cyanescens will have the highest alkaloid content. Smaller mushrooms have more alkaloid % by weight in P. cubensis and there has been success increasing the alkaloid content by adding precursors tryptamine and further downstream.
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I have about 3 different species and 3 cubensis varieties of spores, but for a good solid work I need atleast 5 different species to compare and would like to kindly ask if anyone has spores to donate to for this cause? I am planning to take lots of prints anyway, since I have all the equipment to grow efficently in lab settings so I will spread the love back when I am able.
Species? Or strains?
There should be a link to the sponsors near the top of the forum.
Quote:
The methods of analysis will be liquid and gas chromatography and possibly some kind of capillary method that another local chemistry institute is looking into perfecting.
I thought that was commonplace, now...
Quote:
I am also looking for any advice and any kind of ideas at what I should put into my work, since I have alot of freedom to do as I see fit, because of my previous experience in growing and my supervisor trust's me and is a long time researcher of different psychoactive substances.
You should clone the enzymes tryptophan decarboxylase and after into S. cerveza and get it to make psychedelic beer 
Quote:
i'm not sure what kind of laws estonia has, i know a lot of eastern blok countries are down right draconian with regard to importing mushroom cultures,
Well, this is in an institutional setting and besides, with some of the stuff I have ordered from Estonia I would be surprised if their laws were that harsh. It was either a shady underground chemist that openly advertises on the Internet, or more likely, a legitimate supply company and their laws don't preclude the sale of certain substances. Then again, China is much the same...
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: Chemical analysis of Psilocybe sp. [Re: micro]
#22453494 - 10/30/15 12:48 PM (8 years, 3 months ago) |
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Hi Sven, It's good to hear of your academic studies in this field, it's interesting that you are doing this now as there is a prominent Mycologist doing a very similar work at present. Here is a link to a post regarding his work, it may be beneficial for you to contact him. http://www.shroomery.org/forums/showflat.php/Number/22345076/page/5
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Runegaldr
Stranger
Registered: 10/30/15
Posts: 4
Last seen: 8 years, 2 months
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Re: Chemical analysis of Psilocybe sp. [Re: drake89]
#22465448 - 11/02/15 06:15 AM (8 years, 2 months ago) |
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Quote:
inski said: Hi Sven, It's good to hear of your academic studies in this field, it's interesting that you are doing this now as there is a prominent Mycologist doing a very similar work at present. Here is a link to a post regarding his work, it may be beneficial for you to contact him. http://www.shroomery.org/forums/showflat.php/Number/22345076/page/5
That's amazing, I will be sure to link up with this fellow. Tallinn University has been researching psychedelic mushrooms for a few years now, last year a similar work was done, but the student who did it failed miserably as she did not have the passion for it.
We have dried samples from 2009 and 2014 which will be used to see how they hold potency over longer periods. But the rest of the work will be conducted with freshly grown samples to compare how the methodology of growing affects the contents. 
Quote:
drake89 said: i'm not sure what kind of laws estonia has, i know a lot of eastern blok countries are down right draconian with regard to importing mushroom cultures, much less active spores. My advice would be to start in Amsterdam where they recently started grow 'stones' or truffles. They have several varieties for sale and perhaps you could see if there is a difference between them on a chemical level.
Outside the institute growing is illegal yes, but my university has a license, and the other uni in Tallinn has a license for cannabis.
Edited by Runegaldr (11/02/15 06:31 AM)
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Runegaldr
Stranger
Registered: 10/30/15
Posts: 4
Last seen: 8 years, 2 months
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Re: Chemical analysis of Psilocybe sp. [Re: micro]
#22465484 - 11/02/15 06:38 AM (8 years, 2 months ago) |
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Quote:
micro said:
Quote:
Runegaldr said: My work is to compare different growing methods and species(and varieties) for their alkaloid content and hopefully even some bioactivity testing on various cell cultures.
As far as species, Psilocybe azurescens and Psilocybe cyanescens will have the highest alkaloid content. Smaller mushrooms have more alkaloid % by weight in P. cubensis and there has been success increasing the alkaloid content by adding precursors tryptamine and further downstream.
Species? Or strains?
Quote:
The methods of analysis will be liquid and gas chromatography and possibly some kind of capillary method that another local chemistry institute is looking into perfecting.
I thought that was commonplace, now...
Quote:
I am also looking for any advice and any kind of ideas at what I should put into my work, since I have alot of freedom to do as I see fit, because of my previous experience in growing and my supervisor trust's me and is a long time researcher of different psychoactive substances.
You should clone the enzymes tryptophan decarboxylase and after into S. cerveza and get it to make psychedelic beer 
Quote:
i'm not sure what kind of laws estonia has, i know a lot of eastern blok countries are down right draconian with regard to importing mushroom cultures,
Well, this is in an institutional setting and besides, with some of the stuff I have ordered from Estonia I would be surprised if their laws were that harsh. It was either a shady underground chemist that openly advertises on the Internet, or more likely, a legitimate supply company and their laws don't preclude the sale of certain substances. Then again, China is much the same...
A few cubensis strains. And P. Mexicana, P. Galindoii and one more species that is not cubensis. 
Gas chroma seems to fail miserably and only do damage to the machine. LC is pretty common yes, but the extraction solution changes the results alot.
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There should be a link to the sponsors near the top of the forum.
I know I can order them, but I was hoping for people with good hearts to help out, but yeah, I guess being a stranger and all doesn't really help with peoples generosity.
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fipsy
Stranger
Registered: 05/13/07
Posts: 126
Last seen: 3 years, 2 months
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Re: Chemical analysis of Psilocybe sp. [Re: Runegaldr]
#22466732 - 11/02/15 01:52 PM (8 years, 2 months ago) |
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i would be interested about potency differencis, if grown on walnut,cause they are rich in serotonin
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Chemical analysis of Psilocybe sp. [Re: fipsy]
#22469232 - 11/03/15 01:00 AM (8 years, 2 months ago) |
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How would it undergo a 5 to 4 hydroxy rearrangement without an adjacent carbonyl?
Then again, it's enzymes 
Who knows what the hell the do?
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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