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Subnet Mask



Registered: 05/08/15
Posts: 903
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How do these plates look?
#22451636 - 10/29/15 10:41 PM (8 years, 3 months ago) |
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Greetings all
These are the first transfers I've ever made. They came from one MS plate. I can see some contams but it's hard for my noob eyes to tell what is and what isn't. Can y'all professionals help me out?




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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Loc: to the brain
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Re: How do these plates look? [Re: Subnet Mask]
#22451709 - 10/29/15 11:11 PM (8 years, 3 months ago) |
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I would pull transfers from 1,3,and 5...transfer a couple pieced to new plates...pieces about a quarter of the size of the original transfer...that strong growth on 4 also looks promising.
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Edited by LocN9ne (10/29/15 11:12 PM)
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Subnet Mask



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Re: How do these plates look? [Re: LocN9ne]
#22451793 - 10/29/15 11:43 PM (8 years, 3 months ago) |
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Do you think 2 looks ok? Does the agar just two tone like that sometimes?
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
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Re: How do these plates look? [Re: Subnet Mask]
#22451797 - 10/29/15 11:44 PM (8 years, 3 months ago) |
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2 looks fucked with bacteria
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Q&A US vs. THEM The more I learn, the less I know.
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
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Re: How do these plates look? [Re: LocN9ne]
#22451901 - 10/30/15 12:38 AM (8 years, 3 months ago) |
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picture 1, 2 and 4 looks fucked with bacteria. Just make a few transfers. Watch your sterile procedures in the SAB.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
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Re: How do these plates look? [Re: Mr. Alien]
#22451934 - 10/30/15 12:54 AM (8 years, 3 months ago) |
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Not completely fucked he could transfer from those other plates, but #2 has no chance.
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ston3y
The Propagator


Registered: 09/28/15
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Agreed, 1,3&5 are the best looking, but you can pull healthy mycelium from all the plates. I would get a SAB and a bunch of fresh agar dishes, transfer 3-1-5-4-2 in that order with transfers the size of a grain of rice. They look great though, just make sure to use good sterile tek.
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mushpunx
Fungus Punk



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Re: How do these plates look? [Re: ston3y]
#22452313 - 10/30/15 05:43 AM (8 years, 3 months ago) |
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Yea the second plate has that sneaky bacteria that covers the whole plate with a film so its hard to know if its there.
Was there a lot of bacteria on the first plate (that these transfers are from)? If you have a photo of it maybe we can help you figure out why you have so much bacteria on these
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 Amateur Mycologists United AMU Q&A
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Subnet Mask



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Re: How do these plates look? [Re: mushpunx]
#22452714 - 10/30/15 08:43 AM (8 years, 3 months ago) |
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Seemed fine to me. This is what it looks like now. I don't have any photos of it when i inoculated.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
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Re: How do these plates look? [Re: Subnet Mask]
#22452763 - 10/30/15 09:02 AM (8 years, 3 months ago) |
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Yeah there's your problem.
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Subnet Mask



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Re: How do these plates look? [Re: Subnet Mask]
#22452791 - 10/30/15 09:13 AM (8 years, 3 months ago) |
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There is a lot of condensation on this plate. Could that be making it look that way? The myc seemed to cover the plate quickly
Maybe I'm just in denial. lol
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mushpunx
Fungus Punk



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Re: How do these plates look? [Re: Subnet Mask]
#22452847 - 10/30/15 09:29 AM (8 years, 3 months ago) |
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Quote:
Subnet Mask said:

Seemed fine to me. This is what it looks like now. I don't have any photos of it when i inoculated.
Did you inoculate with a spore syringe? The liquid kinda slides around and if it germinates everywhere it can grow over mold or bacteria. Same as if you let the germination plate grow out big before you transfer, its the most likely plate to be dirty.
Next time drop spore solution onto a sterile cotton swab and swipe a little spot on the agar. Or a flame sterilized loop. Take your first transfer as soon as you see healthy growth as far away from contams as you c an get it, maybe wait till its grown out about the size of a dime or so.
You only need 1 transfer from the first plate anyways, you wont see any sectoring for at least 3 or 4 transfers, usually like 8 or 9 to see prominent sectoring IME. But from the first couple plates you are just cleaning up the culture, so just take a transfer from the healthiest looking spot
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Subnet Mask



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Re: How do these plates look? [Re: Subnet Mask]
#22452853 - 10/30/15 09:31 AM (8 years, 3 months ago) |
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Yes it was with Syringe. Thank you for showing me the error of my ways. You guys rock!
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PussyFart
Retired Cultivation Extrodinaire



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Re: How do these plates look? [Re: mushpunx]
#22452884 - 10/30/15 09:44 AM (8 years, 3 months ago) |
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Quote:
mushpunx said: You only need 1 transfer from the first plate anyways
I like doing 2 or 3 transfers just to be sure it's clean, but that's just me.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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mushpunx
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Re: How do these plates look? [Re: PussyFart]
#22452968 - 10/30/15 10:10 AM (8 years, 3 months ago) |
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Quote:
PussyFart said:
Quote:
mushpunx said: You only need 1 transfer from the first plate anyways
I like doing 2 or 3 transfers just to be sure it's clean, but that's just me.
Oh me too dude! I mean he only really needs to taket one wedge from the germination plate instead of starting like 5 plates
I usually do at least 3 beforr I go to grain cuz Im paranoid
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 Amateur Mycologists United AMU Q&A
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