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OfflineYaMoonSun
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Registered: 10/23/14
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Corners that cut back
    #22448379 - 10/29/15 08:57 AM (8 years, 3 months ago)

So about a week ago I decided to make a spore syringe, and during the steam sterilization of my shot-glass, some moisture appears to have gotten into the shot-glass. Rather then start over, I got frustrated and made the syringe anyways. I've had to toss eight substrates this week.


Don't cut corners.. It's fucking stupid, and you're only hurting yourself.

Made a SAB last night, and hoping to start a fresh syringe this afternoon. :facepalm3:


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Offlinenewrook
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Re: Corners that cut back [Re: YaMoonSun]
    #22448401 - 10/29/15 09:05 AM (8 years, 3 months ago)

Steam sterilize a shot glass? wut

Probably had more to do with the "making it in open air" than whatever moisture you're talking about.


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InvisibleInocuole
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Re: Corners that cut back [Re: newrook]
    #22448403 - 10/29/15 09:06 AM (8 years, 3 months ago)

Don't you uh... put water in the shotglass to make the syringe anyway?  Wouldn't a little water in there be perfect?  Maybe not understanding..


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OfflineYaMoonSun
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Re: Corners that cut back [Re: newrook]
    #22448409 - 10/29/15 09:08 AM (8 years, 3 months ago)

It was dry before I put it into the steam, but I put it upside down so the steam wouldn't get into the glass, and ironically enough it did anyways. A warm moist place is the perfect breeding ground for bacteria, innit? Generally I just sanitize using some rubbing alcohol, and I've never had a problem, but I wanted to try doing things by the book, and I fucked up I guess. Unless this house is just filthy or something.


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InvisibleInocuole
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Re: Corners that cut back [Re: YaMoonSun]
    #22448422 - 10/29/15 09:12 AM (8 years, 3 months ago)

I don't think the inside of a PC is a good breeding ground for bacteria though, so it was never an issue I imagine.  Did you just cover them in foil?  I would wrap it in foil and put it inside of a pp5 container if you have one big enough, and sterilize that.  I have a few tall pasty plate type containers that would be perfect for doing that.  That way the pp5 is what's keeping it sterile until you open it in a SAB, and the foil is just a little extra security for when you're handling it.


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OfflineYaMoonSun
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Re: Corners that cut back [Re: Inocuole]
    #22448435 - 10/29/15 09:15 AM (8 years, 3 months ago)

Personally, I sterilize the syringe and water for the syringe separately from the shot glass. Generally I only sanitize the shot-glass. This was my first attempt at getting it sterile, and it kind of back-fired as far as I can tell. The pp5 doesn't sound like a bad idea though, I was considering that earlier while I was wrapping the shot-glass. The water should just be steaming up here shortly.


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OfflineDrCrumbs
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Re: Corners that cut back [Re: YaMoonSun]
    #22448436 - 10/29/15 09:16 AM (8 years, 3 months ago)

I PC mine with other tools, and the tools still get flamed.

Edit: Sounds like he held it in steam?

Right and wouldnt the water in the PC be sterilized?

Might contain some nutes from other cooks.

Edit: oh, it was PC'ed. Prob not your issue then.


Edited by DrCrumbs (10/29/15 09:17 AM)


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OfflineYaMoonSun
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Re: Corners that cut back [Re: DrCrumbs]
    #22448450 - 10/29/15 09:20 AM (8 years, 3 months ago)

Technically it was steam sterilized in a large cooking pot; I don't have a pressure cooker :shrug:

Think I need to customize the SAB to contain some LED lights too, as I can't see a damn thing in there.


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OfflineDrCrumbs
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Re: Corners that cut back [Re: YaMoonSun]
    #22448459 - 10/29/15 09:24 AM (8 years, 3 months ago)

That could be why then yeah.

When I was doing a lot at once, I've had to sanitize glasses with iso when I ran out of shot glasses and that has worked fine.


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OfflineYaMoonSun
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Registered: 10/23/14
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Re: Corners that cut back [Re: DrCrumbs]
    #22448480 - 10/29/15 09:30 AM (8 years, 3 months ago)

I should probably turn off the stove then and just do what works for me until I get a damn PC :shrug:


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