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Offlinenewrook
Sucks at bulk
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Registered: 03/20/15
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Pf Tek - From spore to bulk and the elimination of the SGFC * 5
    #22447136 - 10/28/15 10:52 PM (8 years, 3 months ago)

Skills Necessary:

Basic knowledge of agar -
(this is required only if you want to clean up your spore syringe on agar but the benefits are HIGHLY RECOMMENDED.
Would you do an hours work now to save working a month straight later? This is that kind of situation)

--

Pasty Plates

http://www.shroomery.org/forums/showflat.php/Number/19208976

credits to PastyWhyte

--

Monotub Tek

http://www.shroomery.org/forums/showflat.php/Number/20356759

credits to SpitballJedi

====================================================================================================

Your Checklist:

Vermiculite
Whole Brown Rice
Wide Mouth Half Pints
Spores / Spore Syringe
Mixing Bowls
Lighter or other flame sterilization tool
Tinfoil
Gloves

Iso (70%)
Paper Towel
Polyfil
Coffee Grinder
Strainer
A Big Bucket
Coir
Gypsum
(optional)
Pressure Canner (you can steam them too, but just spend the money.)
Drill or Hammer + Small Nail
Your Tubs (anything really, I like 60-70qt the best)
Garbage Bags (I use painters drop sheets to make my liners)
Drill + 1.5 inch hole saw ( or something to make 1.5inch dia holes with )

--
Still Air Box                        (If you arn't going straight spore syringe to jar)
Pasty/Other Agar Plates (If you arn't going straight spore syringe to jar)
Syringe of Sterile Water  (If you arn't going straight spore syringe to jar)

====================================================================================================

1) Clean up your spores on agar.

((SKIP TO STEP TWO IF YOU ARE DOING A STRAIGHT MS SPORE SYRINGE GROW))

While following your own personal sterile technique take a drop of spore
solution or a small amount of spores on a sterile tool or inoculation loop
and put it on your pasty plate inside of your still air box.



Put the plate in a safe place :smile:.


As soon as you start to have good clean growth you are going to want to
transfer. If you don't mind doing an MS grow and just want to make sure the
spores are clean you can keep growing out the plate you started with and
later turn it into your LI(liquid inoculate).

Start

Bacteria


One transfer


I transfer until I have clean and even growth, and then let it colonize the plate about 80%. Then you are ready to make your LI.


(agar ready to be made into LI)

In your cleaned and prepped SAB, have your colonized plate ready to go.



Flame your needle of the syringe of sterile water and wipe it with an alcohol wipe.



Pop the lid on your plate and first "pressure wash" the colonized area using
a bit of the water and some pressure from the syringe.



Then use the needle tip to slurry the entire top layer of your plate, and draw what you can back up into the syringe. Do this a few times until you have a good amount of plate inside your syringe.



((Use gloves for this process please))

I like to use 30 cc syringes with a slightly higher gage tip to make it
easier to draw the slurry back into the syringe. This stuff is great though,
you will be impressed with the speed of it. One drop per inoc point. It
last's a while in the fridge too.

====================================================================================================

2) Make your jars.



These videos are readily available online but here you go:

The only mod you make to the jars is 4 inoculation points with the hammer and
nail. I like the plastic lids, and I used a drill.



The recipe is 1 cup brown rice flour, 1 cup water, 2 cups vermiculite for 4
cakes. Multiply this for however many you need.



Take your whole brown rice and grind it up in the coffee grinder. Make it
pretty fine but no need to go overboard.



Measure and add to mixing bowl with the water and vermiculite and mix it up
well by hand. I like to use a sort of scoop and sift motion to mix it all up
well, make sure you get all the fine stuff out of the very bottom and sides.
You want this mix light and fluffy, you shouldn't be compacting it at all.



Add this to your half pints til you're a half inch from the top. Try not to
be messy, you're going to have to wipe down the half inch of rim you have
left with a dry paper towel.



Add your dry vermiculite barrier. Don't go too overboard here either or the
lid is going to compact it down when you put it on your jar.



Wrap the top in tinfoil



Pressure Cook(buy a pc, check craigslist or something) / Steam Sterilize)
1hr45m at 15 psi is what I do.



Let them cool overnight and then move them over to your SAB. You can wipe
down the bottoms quick with a paper towel because they might be a little bit
wet.

====================================================================================================

3) inoculation

Flame your needle tip outside the SAB and wipe it with an alcohol wipe in
between jars and before you start. Also prep your SAB and follow your sterile
procedures.



Remove the foil one at a time and insert the syringe and do a drop of
solution per hole.

Wow that was tough.

====================================================================================================

4) The waiting game

Make your monotubs during this time

http://www.shroomery.org/forums/showflat.php/Number/20356759

credits to SpitballJedi

I don't go as high tek with the magnetic covers et al but you definitely want
a liner.

Jars Colonizing


When they are done you need to make your CVG (coir verm gypsum)

====================================================================================================

5) The CVGv's

1 Brick Coir, 2 Quarts Verm, 5 Quart rolling, boiling water (cheaper coir uses less water ime, I use 4.5 quarts on my brand.)


Put your coir brick in a bucket

Put 2 quarts verm in the same bucket



Put 4-5 quarts BOILIN water in bucket




Cover the bucket with a lid and let it sit for 2 hours wrapped in a blanket to retain heat.



Mix up, and add a handful of Gypsum and mix again.

Let it sit wrapped up until the next day. Do not mix your spawn with hot cvg.

====================================================================================================

6) Onto the Tubs

When your mix has cooled, it's time to prep your fully colonized PF jars.

Crack em open and remove the dry verm.



Take your cheese grater (I use the
biggest hole pattern) and shred em all up.



Add water and let soak for 10-15 mins.

Strain off.



Take all but a small amount of your bulk sub that you're going to use (leave
a bit for a casing layer) and put it into a fresh garbage bag.

Add your shredded, hydrated, cakes.



Shake it like a newborn baby.



Add to desired level in your monotub.

******I now put a medium amount of polyfil into all holes at spawning and don't wait for colonization******

====================================================================================================

7) Again with the waiting.

Wait for 100% colonization. With this method, this actually happens usually
within 48 hours even with a small amount of spawn because of how finely
grated it ends up.


(this is after 24 hrs recovery, 2.5qts spawn to a 70qt tub)

At 100% you can either case it or fruit it now. I recommend casing.
(1/4 inch casing layer)

(minitub)

If you do case you put the lid back on and wait for the casing to be about
30% colonized, you then patch the colonized parts with a bit more casing and
put into fruiting conditions.


(this tub is ready to be patched and fruited)

====================================================================================================

8) You are home free.

You will learn to recognize what your shrooms need and when, whether or not
you should mist and things of that nature. But this is pretty much the part
where you sit back and wait for them to start popping up.

I think grains still throw out better shrooms overall.

I threw out my SGFC, but I still like to do cakes. Pretty foolproof.

Thanks to everyone at the shroomery who has helped me.

Let me know if I need to add anything.



(EQ Multispore) (5 half pints spawn)

--
(EQ cleaned up on agar)(10 half pints spawn)

--
(B+ Clone)(11 half pints spawn)

--
(B+ Multispore)(12 half pints spawn)



--------------------

                                                                      THROW AWAY YOUR SGFC


Edited by newrook (12/04/15 07:22 PM)


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
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Re: Pf Tek - From spore to bulk and the elimination of the SGFC (UNDER CONSTRUCTION) [Re: newrook] * 1
    #22501023 - 11/09/15 04:02 PM (8 years, 2 months ago)

:hehehe:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleShroomspective
Looking into the void
I'm a teapot User Gallery


Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
Re: Pf Tek - From spore to bulk and the elimination of the SGFC (UNDER CONSTRUCTION) [Re: cronicr]
    #22501032 - 11/09/15 04:04 PM (8 years, 2 months ago)

:asianofapproval:


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OfflineNDStepp84
Stellar nuclear waste
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Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
Re: Pf Tek - From spore to bulk and the elimination of the SGFC (UNDER CONSTRUCTION) [Re: Shroomspective]
    #22572005 - 11/25/15 09:22 AM (8 years, 2 months ago)

:congrats: Nice write up. I was thinking the other day it would be great if someone did one on spawning cakes to bulk. It's a very good transition for someone who wants to try their hand at bulk, liking the LI also:super:


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineMrTrips31
1st timer
Male


Registered: 11/11/15
Posts: 132
Loc: Earth
Last seen: 2 years, 4 months
Re: Pf Tek - From spore to bulk and the elimination of the SGFC (UNDER CONSTRUCTION) [Re: NDStepp84]
    #22596858 - 11/30/15 10:24 PM (8 years, 1 month ago)

Thanks for posting this its going to help me out alot !!!!!


--------------------



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OfflinecronicrFacebook
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Posts: 61,436
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Re: Pf Tek - From spore to bulk and the elimination of the SGFC (moved) [Re: newrook] * 1
    #22610656 - 12/03/15 09:03 PM (8 years, 1 month ago)

This thread was moved from the user's journal.

Reason:
Hope this is what ya meant:lol:


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OfflineGroo
sola dosis facit venenum
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Registered: 12/06/14
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Re: Pf Tek - From spore to bulk and the elimination of the SGFC (moved) [Re: cronicr] * 1
    #22610677 - 12/03/15 09:09 PM (8 years, 1 month ago)

Hands down. Perfect example of what Pro Mycology is all about! :incredible:


Edited by Groo (12/04/15 01:00 AM)


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Offlinenewrook
Sucks at bulk
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Registered: 03/20/15
Posts: 657
Last seen: 3 months, 5 days
Re: Pf Tek - From spore to bulk and the elimination of the SGFC (moved) [Re: Groo]
    #22614360 - 12/04/15 07:19 PM (8 years, 1 month ago)

Quote:

cronicr said:
<img src='https://www.shroomery.org/forums/images/moved.gif'> This thread was moved from the user's journal.

Reason:
Hope this is what ya meant:lol:




Yes, thanks much.

Quote:

Groo said:
Hands down. Perfect example of what Pro Mycology is all about! :incredible:





Lol, thanks Groo. You sure are an interesting one.


--------------------

                                                                      THROW AWAY YOUR SGFC


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OfflineGreenRabbit
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Re: Pf Tek - From spore to bulk and the elimination of the SGFC (moved) [Re: newrook]
    #22620931 - 12/06/15 12:19 PM (8 years, 1 month ago)

Damn you beat me to the write up. This has been my preferred growing method for the past couple years.
Nice job.


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Offlinenewrook
Sucks at bulk
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Registered: 03/20/15
Posts: 657
Last seen: 3 months, 5 days
Re: Pf Tek - From spore to bulk and the elimination of the SGFC (moved) [Re: GreenRabbit]
    #22623850 - 12/06/15 09:55 PM (8 years, 1 month ago)

Quote:

GreenRabbit said:
Damn you beat me to the write up. This has been my preferred growing method for the past couple years.
Nice job.




Thanks... I pretty much just wanted something to show up when people searched pf tek that was a simple guide... but it doesn't show up :haha:


--------------------

                                                                      THROW AWAY YOUR SGFC


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OfflineDrEarnhardt
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Registered: 10/21/15
Posts: 150
Last seen: 8 months, 28 days
Re: Pf Tek - From spore to bulk and the elimination of the SGFC [Re: newrook]
    #22753027 - 01/08/16 02:56 PM (8 years, 23 days ago)



--------------------


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OfflinePsilosoulful
Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Pf Tek - From spore to bulk and the elimination of the SGFC [Re: DrEarnhardt]
    #22753103 - 01/08/16 03:19 PM (8 years, 23 days ago)

Go with the Exo terra plantation soil brick instead.
Less sticks and rocks, and I hear a lot of TC's prefer it over the eco earth brand.


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OfflineDrEarnhardt
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Registered: 10/21/15
Posts: 150
Last seen: 8 months, 28 days
Re: Pf Tek - From spore to bulk and the elimination of the SGFC [Re: Psilosoulful]
    #22754877 - 01/08/16 11:01 PM (8 years, 22 days ago)

Ok awesome. Thank you. I typed in "coir" and there was thousands of choices.


--------------------


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