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 ranman
Stranger
Registered: 09/02/15
Posts: 91
Loc: Canada 
Last seen: 5 years, 2 months
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 Benefits of Liquid Culture? 
#22441824 - 10/27/15 09:57 PM (5 years, 4 months ago) |
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I was recently reading this tek for LCs: http://www.shroomery.org/forums/showflat.php/Number/5238137/fpart/1/vc/1
I don't understand why anyone would go to the hassle of creating LCs instead of inoculating substrate with spore solution.
If I understand correctly, LC inoculation results in faster colonization. But it takes a week to create the LC. So is there any net benefit? Quicker? Less contams? Higher yield? Let me in on the secret.
This is coming from a guy who is no longer a noob, but still learning and asking dumb questions.
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 LocN9ne
ɢﾷﾷd ԲﾷЯ ﾢﾷȚￃΙﾢɢ ﾤﾷ￦ﾤΙԲﾼ
Registered: 04/17/15
Posts: 7,062
Loc: to the brain 
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Re: Benefits of Liquid Culture? [Re: ranman]
#22441833 - 10/27/15 10:00 PM (5 years, 4 months ago) |
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The liquid culture had live mycelium growing in it...so instead of innoculating with spores and waiting for them to germinate and hoping it will be contam free, you will be confident in knowing it's clean inoculant and no germination period... it's already rocking before it ever hits the grains...that being said, if your sterile procedure is not top notch, I wouldn't advise fucking with LC
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Q&A
US vs. THEM The more I learn, the less I know.
Edited by LocN9ne (10/27/15 10:02 PM)
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azur
God of Fuck
Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Benefits of Liquid Culture? [Re: ranman]
#22441834 - 10/27/15 10:01 PM (5 years, 4 months ago) |
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Oh man. You have A LOT to learn.
Inoculation via spores is stupid.
But to answer your question, lc is lightening fast...once you're on a cycle
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micro
bunbun has a gungun
Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Benefits of Liquid Culture? [Re: LocN9ne]
#22441841 - 10/27/15 10:02 PM (5 years, 4 months ago) |
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You can make as much of it as you want...
The week is worth the wait if it means you aren't bound to using spore syringes each time.
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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)
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MarcTheMushroom
MycoNaught
Registered: 03/28/10
Posts: 222
Loc: Canada
Last seen: 4 years, 2 months
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Re: Benefits of Liquid Culture? [Re: ranman]
#22441846 - 10/27/15 10:03 PM (5 years, 4 months ago) |
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The benefit is that once you isolate a good producing strain it can be grown indefinitely either in LC or agar.
With LC you can easily and quickly inoculate multiple jars, bags etc
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ranman
Stranger
Registered: 09/02/15
Posts: 91
Loc: Canada
Last seen: 5 years, 2 months
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Can you use LC with PF tek (i.e., BRF substrate in jars)? Thinking I might give LC a try.
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azur
God of Fuck
Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: Benefits of Liquid Culture? [Re: ranman]
#22441880 - 10/27/15 10:11 PM (5 years, 4 months ago) |
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Definitely
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micro
bunbun has a gungun
Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Benefits of Liquid Culture? [Re: ranman]
#22441882 - 10/27/15 10:11 PM (5 years, 4 months ago) |
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... sure 
you can use it with p much anything
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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)
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Inocuole
Scalpel of Evil's Bane
Registered: 11/22/11
Posts: 24,845
Loc: ★
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Re: Benefits of Liquid Culture? [Re: ranman]
#22441885 - 10/27/15 10:12 PM (5 years, 4 months ago) |
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Quote:
ranman said:
This is coming from a guy who is no longer a noob, but still learning and asking dumb questions.
Slow down there slick.
A clean LC is infinitely more effective and faster than spores. You have to get into agar before you can reliably produce clean LC though.
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Benefits of Liquid Culture? [Re: ranman]
#22441889 - 10/27/15 10:13 PM (5 years, 4 months ago) |
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when LC is clean, is it always faster to inoc grains vs. other methods.
to get a verified clean LC, and maintain it thru time, is usually the hard part.
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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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