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Offlineblaklite
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Registered: 07/13/15
Posts: 2
Last seen: 10 months, 6 days
Golden Oyster liquid culture on straw?
    #22441153 - 10/27/15 05:32 PM (8 years, 3 months ago)

Hi all.  I'm still very new at mushroom cultivation and I thought I'd try my hand at using liquid cultures.  I decided to see if I could get a Golden Oyster liquid culture to grow on pasteurized straw.

I pasteurized the straw and placed into a sterile poly bag (about a gallon in size) and sealed the bag with a thermal sealer.  The bags have ports (and filter patches) in them, so after sterilizing the needle, I injected the culture into the straw.  I placed the bag in a dim, cool (75F) place and waited....and waited....and nothing happened.  After 3 months, it looks like the straw is starting to dry out (evaporation through filter patch), but I see no signs of colonization.

I know that liquid to straw is not the best method, but with the aggressiveness of Oysters, I thought this would work well enough.  Did I do something horribly incorrect or was I just unlucky this time around?  Any help is appreciated.

Thanks!


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Golden Oyster liquid culture on straw? [Re: blaklite]
    #22441307 - 10/27/15 06:10 PM (8 years, 3 months ago)

:popcorn:

Just FYI It doesn't take myc anywhere near 3 months to recover.  Bacteria can take hold a lot faster than the myc can recover, so the sugar in the lc probably caused a bacteria to take off, which inhibited the myc from recovering.  Next time post in the gourmet and medicinal forum.

:cheers:


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Golden Oyster liquid culture on straw? (moved) [Re: blaklite]
    #22441376 - 10/27/15 06:27 PM (8 years, 3 months ago)

This thread was moved from Mushroom Cultivation.

Reason:
:hehehe:


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Offlinedrake89
Mushroom Magnate
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Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
Trusted Cultivator
Re: Golden Oyster liquid culture on straw? (moved) [Re: cronicr]
    #22441506 - 10/27/15 06:46 PM (8 years, 3 months ago)

i've seen commercial growers do this.  but don't use a filter patch bag.  they need more air.


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Fiery Fungi (like us on faeboo)


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Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Golden Oyster liquid culture on straw? (moved) [Re: drake89]
    #22441601 - 10/27/15 07:06 PM (8 years, 3 months ago)

What would you recommend?  :mushroom:


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Offlinedrake89
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Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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Re: Golden Oyster liquid culture on straw? (moved) [Re: invitro]
    #22442174 - 10/27/15 09:04 PM (8 years, 3 months ago)

not golden oyster.  blue oyster.  there was a thread i saw on here.  look it up.


--------------------
Fiery Fungi (like us on faeboo)


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