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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Lids ok for making LC? [Re: micro]
#22441016 - 10/27/15 04:54 PM (8 years, 3 months ago) |
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If your talking about a sterilizer unit or something that holds a vacuum then your right about the contams not being able to get in, but most people use a presto with a rocker that does let unsterile air in and wont hold a vacuum, and as THAT unsterile air goes in sideways to depressurize the jar -it IS a contam vector-. It is bad advice to give to someone using a presto to loosen the lid, though the chances might be remote, I repeat it is bad advice if it's not necessary and it's not necessary.
Edited by invitro (10/27/15 04:55 PM)
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Lids ok for making LC? [Re: pitbullfan]
#22441023 - 10/27/15 04:55 PM (8 years, 3 months ago) |
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Just let everything cool overnight, and don't get your filter wet when drawing up inoculate.
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Lids ok for making LC? [Re: invitro]
#22441033 - 10/27/15 04:58 PM (8 years, 3 months ago) |
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None of those bottles at lab depot inc have SFDs siliconed onto the lid.... check the picture, of course you have to loosen the lid, and ofcourse they are using a sterilizer not a presto....
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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So I pced a quart of water until 10psi, and used a lid almost identical to the OPs, stuck an empty syringe in, and the needle did not get sucked in at all, and the lid popped right off, zero pressure. It is totally not happening that the sfd will allow a vacuum of any kind. SFDs and Cellulose disks pull a ton of air, they are not the same as filter patches on bags.
I took the temp of the water immediately after the syringe going in and it read about 49.5C or 111f I could test again at higher temperatures ....
Edited by invitro (10/27/15 05:49 PM)
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enlightened seed
Utopia is a state of mind



Registered: 05/04/07
Posts: 2,117
Loc: amongst civilization
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Re: Lids ok for making LC? [Re: invitro]
#22441160 - 10/27/15 05:33 PM (8 years, 3 months ago) |
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i like to put a couple of marbles in a jar of LC.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Lids ok for making LC? [Re: invitro]
#22441935 - 10/27/15 08:22 PM (8 years, 3 months ago) |
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Quote:
invitro said: None of those bottles at lab depot inc have SFDs siliconed onto the lid.... check the picture, of course you have to loosen the lid, and ofcourse they are using a sterilizer not a presto..
An autoclave is really just a large pressure cooker that locks.
And yeah, cellulose filters are made for separating solids in suspensions.
It's probably enough to keep contamination away but I'd always ask for the .2um ones.
Quote:
invitro said: I could test again at higher temperatures ....
Did you do the normal sterilization run?
If so, nothing more to demonstrate unless you had the same jars as he is using.
It's safe to say if he has the same filter papers you are using it should be similar.
Quote:
enlightened seed said: i like to put a couple of marbles in a jar of LC.
Yeah, or broken glass or something.
Just need to chop up that mycelium.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Lids ok for making LC? [Re: micro]
#22441976 - 10/27/15 08:31 PM (8 years, 3 months ago) |
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I haven't done the normal run yet no... not sure I have time for that right now.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Lids ok for making LC? [Re: micro]
#22443655 - 10/28/15 07:44 AM (8 years, 3 months ago) |
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I'm talking about the cellulose filter discs from Aloha that are made for mycology.
Also, an easy way to tell how well your sfd or whatever breathes enough not to hold pressure is simply to put the lid on the jar tight, with the jar nearly full of water and take a 60cc syringe. Aspirate. If there were an issue you wouldn't be able to fill the whole syringe, aspirate over and over again if you like, no vacuum forms. Alternatively you could inject 60ccs of air over and over, no resistance.
I just pumped 60ccs of air into a jar 95% full of water, injected all at once in about 1 second, what happens is the lid bows up and then bows back down after one second as the pressure is totally equalized. My sfds are from MadSeason if I recall.
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