Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Lids ok for making LC? [Re: micro]
    #22441016 - 10/27/15 04:54 PM (8 years, 3 months ago)

If your talking about a sterilizer unit or something that holds a vacuum then your right about the contams not being able to get in, but most people use a presto with a rocker that does let unsterile air in and wont hold a vacuum, and as THAT unsterile air goes in sideways to depressurize the jar -it IS a contam vector-.  It is bad advice to give to someone using a presto to loosen the lid, though the chances might be remote, I repeat it is bad advice if it's not necessary and it's not necessary.


Edited by invitro (10/27/15 04:55 PM)


Extras: Filter Print Post Top
InvisibleGrey
⇜ ✯ ⇝
 User Gallery


Registered: 11/06/14
Posts: 6,223
Trusted Cultivator
Re: Lids ok for making LC? [Re: pitbullfan]
    #22441023 - 10/27/15 04:55 PM (8 years, 3 months ago)

Just let everything cool overnight, and don't get your filter wet when drawing up inoculate. :thumbup:


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Lids ok for making LC? [Re: invitro]
    #22441033 - 10/27/15 04:58 PM (8 years, 3 months ago)

None of those bottles at lab depot inc have SFDs siliconed onto the lid.... check the picture, of course you have to loosen the lid, and ofcourse they are using a sterilizer not a presto....

:waitingpatiently:


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Lids ok for making LC? [Re: sir_longdong]
    #22441147 - 10/27/15 05:30 PM (8 years, 3 months ago)

So I pced a quart of water until 10psi, and used a lid almost identical to the OPs, stuck an empty syringe in, and the needle did not get sucked in at all, and the lid popped right off, zero pressure.  It is totally not happening that the sfd will allow a vacuum of any kind.  SFDs and Cellulose disks pull a ton of air, they are not the same as filter patches on bags.

I took the temp of the water immediately after the syringe going in and it read about 49.5C or  111f
I could test again at higher temperatures ....


Edited by invitro (10/27/15 05:49 PM)


Extras: Filter Print Post Top
Invisibleenlightened seed
Utopia is a state of mind
Male User Gallery


Registered: 05/04/07
Posts: 2,117
Loc: amongst civilization
Re: Lids ok for making LC? [Re: invitro]
    #22441160 - 10/27/15 05:33 PM (8 years, 3 months ago)

i like to put a couple of marbles in a jar of LC.


Extras: Filter Print Post Top
Invisiblemicro
bunbun has a gungun
Male User Gallery


Registered: 05/09/03
Posts: 7,532
Loc: Brick City Flag
Re: Lids ok for making LC? [Re: invitro]
    #22441935 - 10/27/15 08:22 PM (8 years, 3 months ago)

Quote:

invitro said:
None of those bottles at lab depot inc have SFDs siliconed onto the lid.... check the picture, of course you have to loosen the lid, and ofcourse they are using a sterilizer not a presto..




An autoclave is really just a large pressure cooker that locks.

And yeah, cellulose filters are made for separating solids in suspensions.

It's probably enough to keep contamination away but I'd always ask for the .2um ones.

Quote:

invitro said:
I could test again at higher temperatures ....




Did you do the normal sterilization run?

If so, nothing more to demonstrate unless you had the same jars as he is using.

It's safe to say if he has the same filter papers you are using it should be similar.

Quote:

enlightened seed said:
i like to put a couple of marbles in a jar of LC.




Yeah, or broken glass or something.

Just need to chop up that mycelium.


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Lids ok for making LC? [Re: micro]
    #22441976 - 10/27/15 08:31 PM (8 years, 3 months ago)

I haven't done the normal run yet no... not sure I have time for that right now. 

:popcorn:


Extras: Filter Print Post Top
Offlineinvitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Lids ok for making LC? [Re: micro]
    #22443655 - 10/28/15 07:44 AM (8 years, 3 months ago)

I'm talking about the cellulose filter discs from Aloha that are made for mycology.

Also, an easy way to tell how well your sfd or whatever breathes enough not to hold pressure is simply to put the lid on the jar tight, with the jar nearly full of water and take a 60cc syringe.  Aspirate.  If there were an issue you wouldn't be able to fill the whole syringe, aspirate over and over again if you like, no vacuum forms.  Alternatively you could inject 60ccs of air over and over, no resistance. 

I just pumped 60ccs of air into a jar 95% full of water, injected all at once in about 1 second, what happens is the lid bows up and then bows back down after one second as the pressure is totally equalized.  My sfds are from MadSeason if I recall.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Injection Grain Bag   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* 8 oz snap on lid jars sirJ 1,061 4 06/26/02 08:35 PM
by Roadkill
* Difficult to get jar lid off after PCing
( 1 2 all )
flyhighfunguy 1,203 22 03/22/18 01:42 PM
by Psilosopherr
* Simple Way to Remove the Wax Seal on Metal Lids
( 1 2 3 all )
Ziran 1,132 45 03/23/18 07:45 AM
by plurfekt
* Re: Making Spore Prints scribbles 1,497 3 04/05/01 03:46 PM
by Cableface
* Do you want to make rye shakables? TrippinRhino 5,393 11 10/11/01 01:56 PM
by TrippinRhino
* Making jars ahead SwondPooping 754 2 09/10/02 11:47 AM
by Ripple
* Re: White plastic jar lids night trainV 1,680 8 04/10/01 10:54 PM
by tom
* autoclave help please heathadelic 1,346 8 08/14/01 02:50 AM
by heathadelic

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
638 topic views. 30 members, 203 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.009 seconds on 14 queries.