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sir_longdong
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Registered: 09/06/15
Posts: 87
Last seen: 1 year, 24 days
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Lids ok for making LC?
#22440599 - 10/27/15 02:56 PM (8 years, 3 months ago) |
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1) wondering if these lids are ok for making karo/water LC?
2) if so will i have to use a filter syringe or will my filter be enough ( think I'm asking to be flamed for such a stupid question)??
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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looks like the lids I use for LC  just make sure you don't tilt the jar so much to get the filter you do have wet.
Inoculate with agar wedge(don't use spore syringe to inoculate LC), and let colonize, when ready you can suck it up with your syringes any time you need.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Yeah, just make sure to loosen the lids a bit if you are pressure cooking them, and after let the entire thing cool down (along with the jars inside) before opening it.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Lids ok for making LC? [Re: micro]
#22440614 - 10/27/15 03:01 PM (8 years, 3 months ago) |
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why loosen the lids if they have a filter? I have loosened 0 lids ever, that have had filters.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Lids ok for making LC? [Re: bodhisatta]
#22440627 - 10/27/15 03:05 PM (8 years, 3 months ago) |
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Probably don't need to, but just to be safe.
I'm not sure how much air can escape if those are .2um filters, but probably enough to prevent them from exploding.
I'd hate to tell someone otherwise and then damage their PC 'cause that has happened to me before
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Lids ok for making LC? [Re: micro]
#22440635 - 10/27/15 03:07 PM (8 years, 3 months ago) |
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they wouldn't explode even without the filter, how do you think mason jars work. but the filters we put on jars flow enough air though.
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sir_longdong
Stranger
Registered: 09/06/15
Posts: 87
Last seen: 1 year, 24 days
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Re: Lids ok for making LC? [Re: bodhisatta]
#22440651 - 10/27/15 03:13 PM (8 years, 3 months ago) |
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awesome good to hear, so since their is basically a filtered hole i don't need a second syringe to help with the vacuum effect correct? i can just suck it up with one syringe and the "covered hole" will replace the polyfille syringe
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Lids ok for making LC? [Re: bodhisatta]
#22440655 - 10/27/15 03:14 PM (8 years, 3 months ago) |
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Quote:
bodhisatta said: they wouldn't explode even without the filter, how do you think mason jars work. but the filters we put on jars flow enough air though.
Oh? Is this a trick question 
They work like jars :V
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Quote:
sir_longdong said: awesome good to hear, so since their is basically a filtered hole i don't need a second syringe to help with the vacuum effect correct? i can just suck it up with one syringe and the "covered hole" will replace the polyfille syringe
Not if you let it cool down completely, first.
(that is the very reason I mentioned it in my post)
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
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Re: Lids ok for making LC? [Re: micro]
#22440674 - 10/27/15 03:20 PM (8 years, 3 months ago) |
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I've never had a jar crack from the vacuum effect, that's very rare scenario IMO.
The filters let a lot of air pass. Enough to depressurize a jar in about 1 second (I timed it ).
I wouldn't hesitate to trust a sfd to be able to depressurize. I would never loosen the lid to pc.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Lids ok for making LC? [Re: invitro]
#22440681 - 10/27/15 03:23 PM (8 years, 3 months ago) |
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filter or not all jars die eventually from heat processing they're not made out of awesome glass.
but most of the people on the internet bitching about their cracked canning jars probably also failed to include their operator error with their inquiry.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Lids ok for making LC? [Re: invitro]
#22440687 - 10/27/15 03:24 PM (8 years, 3 months ago) |
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I do my grain jars with the lid screwed until there's a bit of resistance so I can take the lid off easily. Different strokes lol. I just find it a huge pain when the lids are tight AF
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Lids ok for making LC? [Re: Mad Season]
#22440699 - 10/27/15 03:26 PM (8 years, 3 months ago) |
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I don't go tight AF, but it's not loose or anything. just as torqued down as you would do if it were made entirely of plastic and you didn't want to break it. If you make them tight AF good luck unscrewing them with grace in your SAB or FH
a little bit of chapstick on the top rim of the glass part
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Lids ok for making LC? [Re: invitro]
#22440751 - 10/27/15 03:39 PM (8 years, 3 months ago) |
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Quote:
invitro said: I've never had a jar crack from the vacuum effect, that's very rare scenario IMO.
"its never happened to me so it must be very rare..." 
sorry, i find humor in strange places
the vacuum effect i was referring to is when you *don't* let them cool down
you ever stuck a syringe full of... anything into a bottle like that? :V
Quote:
invitro said: I wouldn't hesitate to trust a sfd to be able to depressurize. I would never loosen the lid to pc.
Well, good luck. The filter patches on the bags they sell hardly allow the air to come out when you squeeze it. It will but it's sloow. Not sure why you think loosening the lids is scary >.>
You aren't supposed to loosen them a lot -- maybe 1/4 turn just so pressurized air can move in and out.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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invitro

Registered: 05/03/13
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Last seen: 1 month, 21 days
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Re: Lids ok for making LC? [Re: micro]
#22440770 - 10/27/15 03:46 PM (8 years, 3 months ago) |
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The reason I said "in my opinion" was specifically to say that I wasn't drawing a hard a fast rule, just telling my experience.
Even a hot lc I think the SFD would depressurize the jar quickly. (I'm running a test right now to confirm)...
I'm not talking about filters on bags, I'm talking about sfds and or celluose filter discs.
loosening the lid is not scary just completely unnecessary and it is a path for contams to come in (however remote the likelyhood the possibility is there), if unfiltered air can come in so can contams.
Edited by invitro (10/27/15 04:11 PM)
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sir_longdong
Stranger
Registered: 09/06/15
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Last seen: 1 year, 24 days
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Re: Lids ok for making LC? [Re: micro]
#22440885 - 10/27/15 04:21 PM (8 years, 3 months ago) |
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the vacuum effect i am referring and asking about is when sucking the LC up the pressure makes it hard for the plunger to move, thats why some lids require a second syringe to create equal pressure. i am asking since this lid basically has a filtered hole am i going to need a second syringe
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
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I see, the answer is no, it will not cause a problem, based on my experiences.
Edited by invitro (10/27/15 04:25 PM)
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Lids ok for making LC? [Re: invitro]
#22440949 - 10/27/15 04:39 PM (8 years, 3 months ago) |
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Quote:
invitro said: loosening the lid is not scary just completely unnecessary and it is a path for contams to come in (however remote the likelyhood the possibility is there), if unfiltered air can come in so can contams.
No, it can't.
Source: I used to work with BL4's =P
But I'll Google it anyway:
Quote:
Remember Dust Falls
If you remember that dust falls or is blown by wind, you will have fewer contamination problems. Thus, a loose foil cover or lid will protect a container from contamination. Loose covers such as those on petri dishes are effective because dust only falls, unless it is blown by a wind. Remember to shut off electric fans when you are working with loose fitting covers. Get your cultures into your dust-free boxes soon as possible.
http://www.disknet.com/indiana_biolab/b024.htm
Quote:
To prevent bottles from shattering during pressurization, the caps of vessels with liquids must be fully loosened before loading. Always use the slow exhaust or liquid cool cycle when autoclaving liquids to prevent liquid boil over which can result in loss of content. Never autoclave a sealed vessel containing liquids as this may result in an explosion of super-heated liquid and steam during the cycle or when the vessel is opened. Wear heat-resistant gloves when opening the autoclave door after a cycle. At a minimum, when removing items from an autoclave, a rubber apron, rubber sleeve protectors and heat-resistant gloves should be worn.
Wait 5 minutes before removing dry glassware from the autoclave, and wait 10 to 20 minutes for glassware containing liquids. The larger the volume of liquid the longer you should wait. Liquids removed too soon may boil and overflow the vessel, burning operator. DO NOT tighten caps on vessels immediately after autoclaving as the vacuum resulting from the cooling of the steam in the vessel can cause the cap or vessel to shatter. This is especially important on larger vessels. Caps with liners may have their liner sucked into the vessel if the cap is not lose enough on the vessel opening.
- See more at: http://www.labdepotinc.com/articles/autoclaving-laboratory-glassware.html#sthash.jWt4RqhW.dpuf
Cellulose filters don't get rid of all microbes and is more of a crapshoot.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Quote:
sir_longdong said: the vacuum effect i am referring and asking about is when sucking the LC up the pressure makes it hard for the plunger to move, thats why some lids require a second syringe to create equal pressure. i am asking since this lid basically has a filtered hole am i going to need a second syringe
just let it cool first
you really should be doing that, anyway
i've had the pressure differential where i stuck a syringe into the jar and it sucked the whole thing up in a second :\
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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pitbullfan
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Re: Lids ok for making LC? [Re: micro]
#22441002 - 10/27/15 04:52 PM (8 years, 3 months ago) |
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If your filter allows airflow (even if its slow) you will not (really cannot) have a vacuum, unless said filter gets clogged somehow.
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