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OfflinePsylocybe
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Grains drying during colonization? What do?
    #22438860 - 10/27/15 04:04 AM (8 years, 3 months ago)

So I've noticed that even without much FAE, after a few days of insane growth, that progress on my grains jars starts to stall and move much slower once the small trickles of excess water start drying up from the sides.

This has gotten worse since I introduced full FAE (a fan) in the room for my monotub (I colonize and spawn in the same room to save on gas), I was seeing  visible progress on my grain jars every day and in the last two days I've barely seen any.

Is there any way to prevent this happening? Like opening the window for longer (it's humid outside anyway) and leaving the fan on for less time? The grain jars are behind a closed cupboard door, with the fan facing the opposite direction from them.


Edited by Psylocybe (10/27/15 04:06 AM)


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InvisibleLocN9ne
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Re: Grains drying during colonization? What do? [Re: Psylocybe]
    #22438868 - 10/27/15 04:10 AM (8 years, 3 months ago)

There is no FAE inside of a colonizing grain jar...the fan shouldn't matter to them.


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Invisibleblindingleaf
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Re: Grains drying during colonization? What do? [Re: LocN9ne]
    #22438884 - 10/27/15 04:20 AM (8 years, 3 months ago)

u don't reallywant ur spawn jars in as humid room.  if the filters get moist, mold can squeeze thru.

i use a large GE hole (3/4"), and a fan is on in my room, and my top grains sometimes dry out, but nothing a shake doesn't take care of.

check ur grain prep.  u might be loading them too dry.
in rare cases, if ur PC rocker is going like crazy thru the cycle, that can dry out jars too IME.


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OfflineMachiavelliavore
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Re: Grains drying during colonization? What do? [Re: Psylocybe]
    #22438943 - 10/27/15 05:15 AM (8 years, 3 months ago)

Did you inoculate with multispore, and how long ago did it germinate?  With multispore syringe inoculation, you expect a sort of big ploom of mycellium around where the spore solution went, followed by slow creeping progress through the rest of the grain.  This is why multispore grain jars are generally shaken between 15-30% colonization.

Drying would occur around the top due to a filter that allows FAE (such as loosely stuffed poly, thin filterdisk material, or too much open surface area.)  What type of filters are you using?

You should not be fanning them or tryin to provide FAE or himdity.  The only interaction with the outside world the grain jars care about is temperature, which would ideally be somewhere between 65-75F.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflinePsylocybe
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Re: Grains drying during colonization? What do? [Re: Machiavelliavore]
    #22439013 - 10/27/15 05:55 AM (8 years, 3 months ago)

Yeah, Multispore. Ah that makes sense! I expected a consistent or expanding growth, didn't think it was normal to slow down!

Noc'd them one and a half to two weeks ago? One is starting to really take hold of the entire grain after a good shake, the rest of them are lagging around 10-15%. (I generally shake around 20-25%, but if 15% is enough I might give 'em a shake to speed things up)

Usually I stuff the polyfill really tightly but this time I stuffed them *slightly* less tightly. I'll remember that next time!

Like I said they're not being directly fanned - I have two cupboard facing each other, one which holds grains and one which holds monotubs. I have a fan facing towards (but not directly at) the opened monotub cupboard, while the grain cupboard stays closed. Still, the fan will be drying out the air around the grains somewhat.


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OfflineKizzle
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Re: Grains drying during colonization? What do? [Re: Psylocybe]
    #22439070 - 10/27/15 06:20 AM (8 years, 3 months ago)

I'm assuming the jars were all made the same way in which case it sounds more like there's bacteria in some of them.


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OfflinePsylocybe
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Re: Grains drying during colonization? What do? [Re: Kizzle]
    #22439078 - 10/27/15 06:29 AM (8 years, 3 months ago)

There were some jars I messed up actually.

What happened was, I forgot to shake 2 or 3 of the jars BEFORE inoculating, so instead I did it after, later finding out that this makes it much more difficult for multispore to spread.

Those are probably the jars that haven't shown any signs of growth thus far, and in one there's a pea-sized speck coming through.


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OfflineMachiavelliavore
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Re: Grains drying during colonization? What do? [Re: Psylocybe]
    #22439088 - 10/27/15 06:37 AM (8 years, 3 months ago)

Well, I was talking about multispore without a shake slowing down a lot.  Ofc shakes go at different speeds depending on how much grain was colonized for the shake and how well you shook to inoculate the entire jar.  Areas in the jar left without colonized grain tend to colonize slowly.

I'd be inclined to think Kizzle is right on the bacteria if it's making next to no progress.

Detail your grain prep.  Again, excessive FAE due to shitty filters will only dry out the top grains.

Not shaking before inoculating isn't likely to have much effect.  Shaking after distributes the spore solution.  Most people prefer it on the edges to see what germnates.  Sounds like a case of dirty inoculant.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflinePsylocybe
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Registered: 10/06/10
Posts: 145
Loc: Scotland
Last seen: 7 years, 3 months
Re: Grains drying during colonization? What do? [Re: Machiavelliavore]
    #22439191 - 10/27/15 07:55 AM (8 years, 3 months ago)

I didn't think bacteria would halt the growing process all-together, I assumed I would just get different strains of bacteria as well as mycelium growing at the same time. That makes figuring out the cause quite difficult.

- Rinsed thoroughly and soaked grains in cold water overnight with 1tbsp gypsum,
- Rinsed again and boiled, let steam boil off for about an hour until slightly moist to the touch but not enough to leave a wet mark on a paper towel,
- Loaded into jars with polyfill and slightly loose tinfoil, PC'd for 90 minutes
- Left to cool, taken out into alcohol-sterilized area, tinfoil taken off and discarded
- Wiped MS syringe with alcohol and then injected directly into jars, at an angle facing the sides
- Realized I was supposed to shake them after inoculating two or three, shake all of them and then inoculate the other three or four (Next time I'll detail on the stickers what I did differently if I do a similar thing again)

The only thing I didn't do which I usually do, is use a flame against the spore syringe as I thought it might be overkill after using alcohol. This might be where I messed up? I didn't do this because I didn't like how using a flame against the needle would inevitably make the solution leak somehow.


Edited by Psylocybe (10/27/15 11:22 AM)


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