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InvisibleGhatti
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Registered: 06/23/14
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Testing an lc
    #22433622 - 10/25/15 08:23 PM (8 years, 3 months ago)

How do you guys test your lc specifically? What I mean is, every time I try to test my lc on a plate I always get growth that looks like a lc drop rolled around.


Should I be dripping a drop onto an inoculation loop and streaking, touching in one tiny spot, just dab the needle point on the agar without pushing at all?


Basically what's the easiest way to inoculate just the tiniest spot?


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InvisibleLocN9ne
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Re: Testing an lc [Re: Ghatti]
    #22433624 - 10/25/15 08:25 PM (8 years, 3 months ago)

Is it bacteria on the agar that you are saying it looks like it rolled around, or does is grow mycelium where the trails are?.


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Invisibleazur
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Re: Testing an lc [Re: Ghatti]
    #22433626 - 10/25/15 08:26 PM (8 years, 3 months ago)

A few ways:
Make your agar softer
Pf cake
Pint jar of grain spawned to mini tub


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InvisiblePastywhyteMDiscord
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Re: Testing an lc [Re: azur]
    #22433639 - 10/25/15 08:28 PM (8 years, 3 months ago)

Melt a divot in the agar with a hot loop or scalpel. Then drop the drop in that.


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InvisibleGhatti
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Re: Testing an lc [Re: Pastywhyte]
    #22433682 - 10/25/15 08:42 PM (8 years, 3 months ago)

It's not bacteria its from my lc rolling about.

I've tried making a divot but I always manage to still spill it out onto the surrounding plate.

Could I do this: suck up lc, flame needle, squirt to quench and just touch the needle point to my plate?

Would it carry enough live culture to do anything if I stab my plate or will it depend on how well my lc is shredded when I try this?


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InvisiblePastywhyteMDiscord
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Re: Testing an lc [Re: Ghatti]
    #22433689 - 10/25/15 08:43 PM (8 years, 3 months ago)

Melt a deeper divot. If you leave it for a day without moving the water often absorbs back into the agar and the culture recovers in one spot.


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InvisibleGhatti
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Re: Testing an lc [Re: Pastywhyte]
    #22433731 - 10/25/15 08:57 PM (8 years, 3 months ago)

Alright I'll give it a go. Maybe just use a thick ass plate next time so I have more of an lc bucket.


Thanks you cooky canuck


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Offlinetripdawg420
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Re: Testing an lc [Re: Ghatti]
    #22433757 - 10/25/15 09:04 PM (8 years, 3 months ago)

i didnt think people still used lc but ya put a drop on the agar and leave it alone dont move it around :shrug:


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epic GT mono tub
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wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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InvisibleGhatti
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Re: Testing an lc [Re: tripdawg420]
    #22433795 - 10/25/15 09:14 PM (8 years, 3 months ago)

Why would people still not use lc?


It's great when its good
Shitty when its bad.


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Offlinetripdawg420
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Re: Testing an lc [Re: Ghatti]
    #22433813 - 10/25/15 09:19 PM (8 years, 3 months ago)

idk why people do :shrug:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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InvisibleLocN9ne
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Re: Testing an lc [Re: Ghatti]
    #22433842 - 10/25/15 09:28 PM (8 years, 3 months ago)

Quote:

Ghatti said:



It's great when its good




That's why.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


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Offlinetripdawg420
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Registered: 02/02/09
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Re: Testing an lc [Re: LocN9ne]
    #22433846 - 10/25/15 09:30 PM (8 years, 3 months ago)

:thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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