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InvisibleKalistis
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Registered: 09/06/15
Posts: 2,265
Golden Teacher Clusters, Cloning, & More. COMPLETE! FINAL YIELD UPDATE * 1
    #22428875 - 10/24/15 07:16 PM (8 years, 8 months ago)

Alright... The monotub that was cracked by my idiot move to attempt to remove water from below the liner has recovered well but it does have some metabolites. Larger fruits than the other tub spawned at the same time.  I'm assuming that the stress pushed them to fruit sooner because the pinset in the other tub came in more even than the cracked tub.

Keep in mind I put my best 6 quart jars of GT spawn in the tub not cracked and 5 of my least favorite jars in the second tub that cracked, so maybe the early fruiting and the metabolites were due to the quality of the spawn?

Per the advice of Pasty, I tightened my bottom polyfil and misted the hell out of these tubs. Temps have remained between 72 and 76 at the warmest. The difference in the number of pins between yesterday and today is overwhelming but because my substrate is uneven the pins are clustering.



UPDATED 10/25/15 PM




More pins keep coming in huge clusters. Crazy.I'm PCing some agar as we speak. I will clone some of these this week.

Edited by Kalistis (11/03/15 04:37 PM)

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OfflineYaMoonSun
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Re: How am I doing? [Re: Kalistis]
    #22429017 - 10/24/15 07:40 PM (8 years, 8 months ago)

They have some weird nips, but congrats nevertheless. I'd mist them, but I'm a misting addict :super:

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InvisibleKalistis
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Re: How am I doing? [Re: YaMoonSun]
    #22429037 - 10/24/15 07:43 PM (8 years, 8 months ago)

I think Pasty said the nips you are referring to are the universal veils.

I just misted them. How do you know if things are too wet?


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InvisibleLocN9ne
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Re: How am I doing? [Re: Kalistis] * 1
    #22429064 - 10/24/15 07:47 PM (8 years, 8 months ago)

Pools of water that don't evaporate or soak in...that'd be too wet.


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Q&A
US vs. THEM

The more I learn, the less I know.

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OfflineYaMoonSun
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Re: How am I doing? [Re: Kalistis]
    #22429075 - 10/24/15 07:50 PM (8 years, 8 months ago)

I'm referring to what looks like mutant titties, there's like little growths on a few of those caps. Also, I'm pretty sure you can get away with a heavy misting at least once or twice a week with a mono, but I've yet to do one, so don't hold me to that, it's just an educated guess

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InvisibleKalistis
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Re: How am I doing? [Re: LocN9ne]
    #22429093 - 10/24/15 07:52 PM (8 years, 8 months ago)

Aren't they cute! I love them so much... I think I just might feel guilty picking them.

How many days out do you guys think I am from finishing my first flush?

And yes, I had asked about the mutant titties and was told it's just the left over universal veil. I thought it was from insufficient misting of FAE. But I'll do whatever I must!


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InvisibleLocN9ne
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Re: How am I doing? [Re: Kalistis]
    #22429116 - 10/24/15 07:55 PM (8 years, 8 months ago)

Your mind is going to be blown by how rapidly that tub just booms... in two days you're gonna look at this picture compared to your tub and be like "oh dang" lol.


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Q&A
US vs. THEM

The more I learn, the less I know.

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OfflineYaMoonSun
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Re: How am I doing? [Re: Kalistis]
    #22429127 - 10/24/15 07:57 PM (8 years, 8 months ago)

Probably two days, three at most, provided you mist them :super:

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InvisibleKalistis
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Re: How am I doing? [Re: YaMoonSun] * 1
    #22431841 - 10/25/15 12:50 PM (8 years, 8 months ago)

It might not be the prettiest lot but I'm almost there guys. They look meaty!
They aren't coming in at the same rate and new pins keep popping up. How the heck will I harvest these without screwing up the immature fruit when the time comes?





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InvisiblePastywhyteMDiscord
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Re: How am I doing? [Re: Kalistis]
    #22431854 - 10/25/15 12:55 PM (8 years, 8 months ago)

I would try to clone some of the fruits outta one of them big clusters. Thats genetics that ya want.

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InvisibleKalistis
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Re: How am I doing? [Re: Pastywhyte]
    #22431952 - 10/25/15 01:28 PM (8 years, 8 months ago)

Yay! A new skill to learn. I'll start reading up on cloning.

Do I wait for the fruits I want to clone to fully mature to clone? Do you have a preferred cloning Tek?

Thanks for all of your help.


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InvisibleKalistis
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Re: How am I doing? [Re: Kalistis] * 1
    #22431964 - 10/25/15 01:31 PM (8 years, 8 months ago)

Here's my other tub. Also a cluster fuck.



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InvisibleKalistis
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Re: How am I doing? [Re: YaMoonSun]
    #22431992 - 10/25/15 01:39 PM (8 years, 8 months ago)

Pasty, do you ever use biopsy punches for cloning? I have different varieties of disposable and autoclaveable biopsy punches that seem like they would be useful.


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InvisiblePastywhyteMDiscord
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Re: How am I doing? [Re: Kalistis]
    #22432276 - 10/25/15 02:55 PM (8 years, 8 months ago)

I tear the fruit apart and pull out tissue with a scalpel or tweezers from the clean centre.  Punching through carries a fair amount of risk for contam. Cloning pins is pretty easy tho as you just drop the pin on the plate and it converts to myc fast and out runs the contams allowing for a clean transfer. Its best to clone the fruits before they mature too much, pins are great. You want fewer cell divisions for a younger culture.

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OfflineYaMoonSun
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Re: How am I doing? [Re: Kalistis]
    #22432285 - 10/25/15 02:57 PM (8 years, 8 months ago)

Grab from the base of the mushroom and twist it back clockwise and then counter clockwise repeatedly until it loosens up.

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InvisibleKalistis
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Re: How am I doing? [Re: YaMoonSun]
    #22432307 - 10/25/15 03:02 PM (8 years, 8 months ago)

Do I need to wipe the pin with alcohol or anything prior to popping it on my plate? I might not have pins to clone by tomorrow though and I don't have any plates ready to go today. I need to mix up a fresh batch of agar and pour plates.


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OfflineYaMoonSun
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Re: How am I doing? [Re: Kalistis]
    #22432313 - 10/25/15 03:05 PM (8 years, 8 months ago)

You break the mushroom in half and take a tissue sample from the inside of the mushroom using a flame sterilized scalpel or knife.

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InvisibleKalistis
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Re: How am I doing? [Re: YaMoonSun] * 1
    #22432396 - 10/25/15 03:28 PM (8 years, 8 months ago)

Gottcha! I'm pumped.

Nice little surprise found in my colonizing Malabar tubs today... Pins. Cute little white pins everywhere in 1 of the 2 tubs. The second tub has a few but not nearly as many as the first. I misted the shit out of them and threw both into fruiting conditions. I can't believe how quickly these guys popped up. I mixed 6 jars of oats to 1 brick coir, 2 quarts verm, and 1 cup gypsum. I did add a thin layer of just the coir/verm mix on top before people told me it wasn't necessary. I think I want to try casing my next tubs.



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OfflineLogicaL ChaosM
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Re: How am I doing? [Re: Kalistis]
    #22432661 - 10/25/15 04:40 PM (8 years, 8 months ago)

Look like an expert already.

Good work and great pics :awesome::thumbup:


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InvisibleKalistis
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Re: How am I doing? [Re: LogicaL Chaos]
    #22433959 - 10/25/15 10:03 PM (8 years, 8 months ago)

Thanks, trust me when I say I appreciate the words of encouragement, but I am FARRRRR from an expert. I will be happy if I get one good flush from these tubs.

Working on making agar right now. I am curious how the pinset on my Malabar tubs are coming, but I don't want to open them.

My jars of Argentina and B+ are nearly fully colonized. They should be ready to spawn by Tuesday. My boyfriend is a little irritated with me right now. I think he is hoping I'll be done with this hobby after the first or second flush. When I pulled out the PC tonight her asked me what the hell I was doing. I am enjoying myself so much and I really don't see the harm.


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