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Kalistis


Registered: 09/06/15
Posts: 2,265
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Golden Teacher Clusters, Cloning, & More. COMPLETE! FINAL YIELD UPDATE 1
#22428875 - 10/24/15 07:16 PM (8 years, 3 months ago) |
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Alright... The monotub that was cracked by my idiot move to attempt to remove water from below the liner has recovered well but it does have some metabolites. Larger fruits than the other tub spawned at the same time. I'm assuming that the stress pushed them to fruit sooner because the pinset in the other tub came in more even than the cracked tub.
Keep in mind I put my best 6 quart jars of GT spawn in the tub not cracked and 5 of my least favorite jars in the second tub that cracked, so maybe the early fruiting and the metabolites were due to the quality of the spawn?
Per the advice of Pasty, I tightened my bottom polyfil and misted the hell out of these tubs. Temps have remained between 72 and 76 at the warmest. The difference in the number of pins between yesterday and today is overwhelming but because my substrate is uneven the pins are clustering.

UPDATED 10/25/15 PM


More pins keep coming in huge clusters. Crazy.I'm PCing some agar as we speak. I will clone some of these this week.
Edited by Kalistis (11/03/15 04:37 PM)
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YaMoonSun
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Re: How am I doing? [Re: Kalistis]
#22429017 - 10/24/15 07:40 PM (8 years, 3 months ago) |
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They have some weird nips, but congrats nevertheless. I'd mist them, but I'm a misting addict
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Kalistis


Registered: 09/06/15
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I think Pasty said the nips you are referring to are the universal veils.
I just misted them. How do you know if things are too wet?
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LocN9ne
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Re: How am I doing? [Re: Kalistis] 1
#22429064 - 10/24/15 07:47 PM (8 years, 3 months ago) |
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Pools of water that don't evaporate or soak in...that'd be too wet.
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YaMoonSun
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Re: How am I doing? [Re: Kalistis]
#22429075 - 10/24/15 07:50 PM (8 years, 3 months ago) |
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I'm referring to what looks like mutant titties, there's like little growths on a few of those caps. Also, I'm pretty sure you can get away with a heavy misting at least once or twice a week with a mono, but I've yet to do one, so don't hold me to that, it's just an educated guess
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Kalistis


Registered: 09/06/15
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Re: How am I doing? [Re: LocN9ne]
#22429093 - 10/24/15 07:52 PM (8 years, 3 months ago) |
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Aren't they cute! I love them so much... I think I just might feel guilty picking them.
How many days out do you guys think I am from finishing my first flush?
And yes, I had asked about the mutant titties and was told it's just the left over universal veil. I thought it was from insufficient misting of FAE. But I'll do whatever I must!
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LocN9ne
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Re: How am I doing? [Re: Kalistis]
#22429116 - 10/24/15 07:55 PM (8 years, 3 months ago) |
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Your mind is going to be blown by how rapidly that tub just booms... in two days you're gonna look at this picture compared to your tub and be like "oh dang" lol.
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Q&A US vs. THEM The more I learn, the less I know.
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YaMoonSun
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Re: How am I doing? [Re: Kalistis]
#22429127 - 10/24/15 07:57 PM (8 years, 3 months ago) |
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Probably two days, three at most, provided you mist them
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Kalistis


Registered: 09/06/15
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Re: How am I doing? [Re: YaMoonSun] 1
#22431841 - 10/25/15 12:50 PM (8 years, 3 months ago) |
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It might not be the prettiest lot but I'm almost there guys. They look meaty! They aren't coming in at the same rate and new pins keep popping up. How the heck will I harvest these without screwing up the immature fruit when the time comes?

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Pastywhyte
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Re: How am I doing? [Re: Kalistis]
#22431854 - 10/25/15 12:55 PM (8 years, 3 months ago) |
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I would try to clone some of the fruits outta one of them big clusters. Thats genetics that ya want.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Yay! A new skill to learn. I'll start reading up on cloning.
Do I wait for the fruits I want to clone to fully mature to clone? Do you have a preferred cloning Tek?
Thanks for all of your help.
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Kalistis


Registered: 09/06/15
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Re: How am I doing? [Re: Kalistis] 1
#22431964 - 10/25/15 01:31 PM (8 years, 3 months ago) |
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Here's my other tub. Also a cluster fuck.
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Kalistis


Registered: 09/06/15
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Pasty, do you ever use biopsy punches for cloning? I have different varieties of disposable and autoclaveable biopsy punches that seem like they would be useful.
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Pastywhyte
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Re: How am I doing? [Re: Kalistis]
#22432276 - 10/25/15 02:55 PM (8 years, 3 months ago) |
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I tear the fruit apart and pull out tissue with a scalpel or tweezers from the clean centre. Punching through carries a fair amount of risk for contam. Cloning pins is pretty easy tho as you just drop the pin on the plate and it converts to myc fast and out runs the contams allowing for a clean transfer. Its best to clone the fruits before they mature too much, pins are great. You want fewer cell divisions for a younger culture.
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YaMoonSun
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Re: How am I doing? [Re: Kalistis]
#22432285 - 10/25/15 02:57 PM (8 years, 3 months ago) |
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Grab from the base of the mushroom and twist it back clockwise and then counter clockwise repeatedly until it loosens up.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Do I need to wipe the pin with alcohol or anything prior to popping it on my plate? I might not have pins to clone by tomorrow though and I don't have any plates ready to go today. I need to mix up a fresh batch of agar and pour plates.
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YaMoonSun
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Re: How am I doing? [Re: Kalistis]
#22432313 - 10/25/15 03:05 PM (8 years, 3 months ago) |
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You break the mushroom in half and take a tissue sample from the inside of the mushroom using a flame sterilized scalpel or knife.
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Kalistis


Registered: 09/06/15
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Re: How am I doing? [Re: YaMoonSun] 1
#22432396 - 10/25/15 03:28 PM (8 years, 3 months ago) |
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Gottcha! I'm pumped.
Nice little surprise found in my colonizing Malabar tubs today... Pins. Cute little white pins everywhere in 1 of the 2 tubs. The second tub has a few but not nearly as many as the first. I misted the shit out of them and threw both into fruiting conditions. I can't believe how quickly these guys popped up. I mixed 6 jars of oats to 1 brick coir, 2 quarts verm, and 1 cup gypsum. I did add a thin layer of just the coir/verm mix on top before people told me it wasn't necessary. I think I want to try casing my next tubs.
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LogicaL Chaos
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Re: How am I doing? [Re: Kalistis]
#22432661 - 10/25/15 04:40 PM (8 years, 3 months ago) |
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Look like an expert already.
Good work and great pics 
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Kalistis


Registered: 09/06/15
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Thanks, trust me when I say I appreciate the words of encouragement, but I am FARRRRR from an expert. I will be happy if I get one good flush from these tubs.
Working on making agar right now. I am curious how the pinset on my Malabar tubs are coming, but I don't want to open them.
My jars of Argentina and B+ are nearly fully colonized. They should be ready to spawn by Tuesday. My boyfriend is a little irritated with me right now. I think he is hoping I'll be done with this hobby after the first or second flush. When I pulled out the PC tonight her asked me what the hell I was doing. I am enjoying myself so much and I really don't see the harm.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



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Re: How am I doing? [Re: Kalistis] 1
#22434016 - 10/25/15 10:21 PM (8 years, 3 months ago) |
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Lmmfao... he's gonna learn today...why don't you wanna open your tub? Open that motherfucker and take a picture.
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Q&A US vs. THEM The more I learn, the less I know.
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Pastywhyte
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Re: How am I doing? [Re: LocN9ne] 1
#22434036 - 10/25/15 10:30 PM (8 years, 3 months ago) |
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HA! Once people get the bug there ain't no going back. Open the tub up and have a peek, won't hurt a thing
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Quote:
Pastywhyte said: Its best to clone the fruits before they mature too much, pins are great. You want fewer cell divisions for a younger culture.
I agree that pins are much more vigorous when cloned compared to mature fruits... but why would cell division be the reason for that? I thought fruits grow from cell enlargement, not cell division?
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Pastywhyte
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Re: How am I doing? [Re: FriedEgg]
#22434702 - 10/26/15 05:07 AM (8 years, 3 months ago) |
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They grow from both. Some species like hericium grow almost exclusively from division. Far as I can tell cubes start out mostly growing from division and water expansion kicks in as it develops. Many think that once the veil tears the growth at that point is almost all water weight. But certainly division is occurring past the point of pin formation.
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rudeboi
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Inocuole
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Quote:
Pastywhyte said: They grow from both. Some species like hericium grow almost exclusively from division. Far as I can tell cubes start out mostly growing from division and water expansion kicks in as it develops. Many think that once the veil tears the growth at that point is almost all water weight. But certainly division is occurring past the point of pin formation.
I share this view.
I'd say it goes from like 90% division and 10% expansion as a pin to 90% expansion and 10% division right as the veil is tearing. That's a guess of course, based on observation and time scale. I'd say producing spores also takes a little something out of the mycelium's vigor for whatever reason.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: Inocuole]
#22435179 - 10/26/15 09:19 AM (8 years, 3 months ago) |
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Okay so I have my plates ready. Just to confirm, can I pick a pin and just throw it on my plate or do I have to take it into my SAB, split it with a sterile blade and extract tissue?
If I do just throw it on the plate, do I wipe it down with alcohol or anything or will the mycelium just take over contaminants? What's the largest pin I want to work with? When I get home today I'll take a new pic and maybe you guys can hell me decide which pins and fruits to clone. These tubs are ridiculous. I might just get a canopy on my first try!!
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Inocuole
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Re: How am I doing? [Re: Kalistis]
#22435195 - 10/26/15 09:23 AM (8 years, 3 months ago) |
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Okay so....
What does "throwing a pin on a plate" mean to you? Where is the plate while this is happening? You only mentioned the SAB in the second option.
Either works but.. not if your first option means you're opening an agar plate in open air, obviously. Another obvious being that the inside of a pin is cleaner than the outside. So, take the risk if you want. Whole pins are more likely to contam than a piece of the inner tissue, but if it's real small then that's what it is.
Don't put iso on the pin, but do wipe the outside of the plates down with it while you're working.
I recommend the biopsy option but.. it's a matter of preference.
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Kalistis


Registered: 09/06/15
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Re: How am I doing? [Re: Inocuole]
#22435479 - 10/26/15 10:52 AM (8 years, 3 months ago) |
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Oh, I intended on using my SAB in either scenario. I was just confused if I should biopsy the pin or just literally put it on the plate. Maybe I'll try both? I poured 50 plates last night as I'm expecting a shipment as well sometime this week. Yay.
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YaMoonSun
The Double Standard


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Re: How am I doing? [Re: Kalistis] 1
#22435482 - 10/26/15 10:54 AM (8 years, 3 months ago) |
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Quote:
50 plates
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
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Damn, how'd I miss this one. Good job!
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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FriedEgg



Registered: 09/22/14
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Re: How am I doing? [Re: Kalistis]
#22436943 - 10/26/15 05:44 PM (8 years, 3 months ago) |
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Quote:
Kalistis said: Oh, I intended on using my SAB in either scenario. I was just confused if I should biopsy the pin or just literally put it on the plate. Maybe I'll try both? I poured 50 plates last night as I'm expecting a shipment as well sometime this week. Yay.
either one will work. my personal preference is to split the stem with my fingers and take a biopsy from the center.
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Kalistis


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Re: How am I doing? [Re: FriedEgg] 1
#22437579 - 10/26/15 08:34 PM (8 years, 3 months ago) |
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I have a dilemma. Most of what I have read about uneven substrate and pinning says to just pick the fruits as they mature, but my fruits are so closely clustered and at such different levels of maturation that the task isn't as simple as it seems. I will surely damage the smaller pins on the outer most edge during this process.
I see that I have 3 options here:
1. Should I just pick most of the large clusters to avoid the near fully mature guys from dropping spores?
2. Do I wait and let the most mature guys fully mature and drop what they will, allowing for the smaller fellows to catch up?
3. Do I selectively and systematically pick those closest to maturity and leave the little guys giving them a chance to catch up over the next 24 hours and pick as they come?
Lastly, are these darker guys going to abort? They are still growing but look funny.

Thanks again guys.
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Inocuole
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Re: How am I doing? [Re: Kalistis]
#22437587 - 10/26/15 08:35 PM (8 years, 3 months ago) |
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I'd just go ahead and do a clean sweep and get it all if it were me. You can do any of the things you said though, it's all up to you.
Not sure if they're aborts, really won't matter much if you pick them in the next 24 hours.
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Kalistis


Registered: 09/06/15
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Re: How am I doing? [Re: Inocuole]
#22437659 - 10/26/15 08:57 PM (8 years, 3 months ago) |
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Clean sweep all out, even the little tiny pins on the perimeter or leave those for next flush? What do I do with those little tiny guys then? Dry them? I have been popping aborts here and there. Fresh mushrooms are delicious, BTW. I get a little head spacey but def. don't trip. I was considering eating some tomorrow on my day off.
I was planning on adding a couple of cups of water to the substrate once I was done instead of doing a full dunk. What do you think?
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Inocuole
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Re: How am I doing? [Re: Kalistis]
#22437675 - 10/26/15 08:59 PM (8 years, 3 months ago) |
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You can leave the really little pins. If something is part of a cluster and it's about to drop spores, I just take the whole cluster, though.
It doesn't hurt yield too bad though to just grab it all. Harvesting large amounts off a tub seems to delay the maturation of the other fruits.
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Kalistis


Registered: 09/06/15
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Re: How am I doing? [Re: Inocuole]
#22438447 - 10/27/15 12:18 AM (8 years, 3 months ago) |
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I made 8 clones prior to harvest of 1 out of the 2 GT tubs. I don't have high expectations there.... I most certainly feel like I had to have contaminated the plates during that process.
As far as the harvest, 420 grams of fruit from the partial flush out of one tub. Since none of the clusters on the sides looked ready to go, I didn't touch them. I figure I can harvest them tomorrow with the other tub. The first batch is in my dehydrator now. 
Is it normal for there to be a full pin set ready to go just below all of these fruit or are those just going to abort?



Hopefully these will be cracker dry by morning so I can get another batch in. The manual says to do mushrooms at 130 for 2 to 4 hours if they are sliced. Based on what I have read, I decided to bump it up to 160 (machine goes up to 165, but I decided not press my luck) for 4 hours. I will heck them then and add time if necessary.
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FriedEgg



Registered: 09/22/14
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Re: How am I doing? [Re: Kalistis]
#22441616 - 10/27/15 07:09 PM (8 years, 3 months ago) |
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good job
i put mine in the dehydrator for 12-14 hours. sometimes longer if needed. it wont hurt.
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Kalistis


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Re: How am I doing? [Re: FriedEgg]
#22447263 - 10/28/15 11:30 PM (8 years, 3 months ago) |
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UPDATE: Here's what they look like this evening. A little bruised, but I am surprised that they haven't taken any time to recover. Even the crack in the substrate in the one tub has a web of mycelium now. I think the number of pins is crazy. The edges blew up overnight. I wonder if the middle is going to abort like mad? I see that some of the pins that were near larger fruits have bruised. They aren't soft yet and I worry about doing more damage to the network if I pick at it. The first flush had some pretty small fruit. These seem to be growing taller.



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Kalistis


Registered: 09/06/15
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Re: How am I doing? [Re: FriedEgg]
#22447267 - 10/28/15 11:31 PM (8 years, 3 months ago) |
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Quote:
FriedEgg said:
Quote:
Kalistis said: Oh, I intended on using my SAB in either scenario. I was just confused if I should biopsy the pin or just literally put it on the plate. Maybe I'll try both? I poured 50 plates last night as I'm expecting a shipment as well sometime this week. Yay.
either one will work. my personal preference is to split the stem with my fingers and take a biopsy from the center.
I followed this advice and it seems to be working like a charm. Thanks!
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Kalistis


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Re: How am I doing? [Re: Kalistis] 1
#22471976 - 11/03/15 04:42 PM (8 years, 2 months ago) |
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Just finished the 3rd flush on one of my 2 GT monotubs. The other monotub was done after 2nd flush (or at least I thought so).
Final yield Monotub #1 MS to WBS to damon tek with gypsum: Dry 5.4 oz Monotub # 2 Same: Dry 7.8 oz
I have no idea how that compares, but damn! I'm not complaining.
Thank you to everyone that helped me along the way. Here's to more successful grows to come.
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Soreidorb
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Re: How am I doing? [Re: Kalistis]
#22472175 - 11/03/15 05:23 PM (8 years, 2 months ago) |
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I definitely wouldn't be complaining either lol. Congrats man.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



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Well Done.
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Q&A US vs. THEM The more I learn, the less I know.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: How am I doing? [Re: LocN9ne]
#22473387 - 11/03/15 09:06 PM (8 years, 2 months ago) |
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Quote:
LocN9ne said: Well Done.
good job. Seems like ya got the bug
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Thanks guys! I can't believe how much I've accomplished in 2 months.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: How am I doing? [Re: Kalistis]
#22473801 - 11/03/15 10:31 PM (8 years, 2 months ago) |
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great job kalistis, what's next?
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Kalistis


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Re: How am I doing? [Re: FriedEgg]
#22473869 - 11/03/15 10:47 PM (8 years, 2 months ago) |
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I have 2 Malabar MS monotubs finishing their first flush. I didn't deviate at all from the first Tek, I just attempted to improve on it. The tubs are doing great.
I just spawned my first MS Argentina transferred to agar then to oats 2 days ago. 5 quart jars to Damon's Tek again, only I didn't lay a top layer when spawning as I'd like to use the next few tubs to learn how to case.
I spawned MS B+ oats to same Tek as above at same time as the agar spawning. Unfortunately, I didn't have a good feeling about quite a few of my MS jars and discarded all by 4 quarts. Better safe than sorry.
Last Tuesday I performed my first clones of the GT first flush. I made first transfers on Sunday and some clones of my Malabar fruit.
I prepared and poured another 30 plates of MEA this evening to prepare for next clone transfers and to get ready to work with some new syringes I picked up last week. I think my next adventure will be with Pan cambodginiensis. At least some agar work to start.
I bet you weren't expecting that. Lol.
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Hook420
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Re: How am I doing? [Re: FriedEgg]
#22473898 - 11/03/15 10:56 PM (8 years, 2 months ago) |
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Edited by Hook420 (11/03/15 10:57 PM)
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: How am I doing? [Re: Kalistis]
#22473934 - 11/03/15 11:07 PM (8 years, 2 months ago) |
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Yea i wasn't expecting you to jump right into pan cambos already but go for it! That's awesome
As far as growing 3 varieties of similar cubes (malabar, b+, GT)... I don't really see the point... they are all so similar. You may find it more interesting to go after different cubes like PE, APE, or AA+. It takes a lot of work to focus on multiple varieties (especially when cloning). I try to choose my varieties as carefully as possible. My list is: GT, APE, PE, Pan Cyan Hawaii, and sometimes AA+ if I'm bored. But it's your hobby and time so do as you please. ( Just my 2 cents )
Next time you have a jar that seems clean but you don't feel 100% confident about, you could try adding a casing layer to your jars and fruit them as is. I just tried this a few days ago. I don't know if it'll work or not yet. But it's fun to play around.
 
good luck! keep it up!
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: FriedEgg]
#22474978 - 11/04/15 08:31 AM (8 years, 2 months ago) |
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I did 3 similar cubes just to get some experience under my belt with the process. I knew nothing about them prior to August, so when I chose my first cubes, I picked what other new cultivators had picked in the past. I have a feeling I'll try a little of everything then settle on a few favorites.
I did get a PE uncut syringe this last week, but the other strains came from a grab bag and all seem to be pretty standard. Where I got my syringes didn't have any of the other varieties in stock.
I thought about letting the jars do their thing, but figured I had so much going at once that it was a little overwhelming to take on. However, I love the top layer idea! Please let me know how your jars turn out. Thanks for the advice!
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