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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: How am I doing? [Re: Kalistis] 1
#22434016 - 10/25/15 10:21 PM (8 years, 3 months ago) |
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Lmmfao... he's gonna learn today...why don't you wanna open your tub? Open that motherfucker and take a picture.
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Q&A US vs. THEM The more I learn, the less I know.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: How am I doing? [Re: LocN9ne] 1
#22434036 - 10/25/15 10:30 PM (8 years, 3 months ago) |
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HA! Once people get the bug there ain't no going back. Open the tub up and have a peek, won't hurt a thing
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Quote:
Pastywhyte said: Its best to clone the fruits before they mature too much, pins are great. You want fewer cell divisions for a younger culture.
I agree that pins are much more vigorous when cloned compared to mature fruits... but why would cell division be the reason for that? I thought fruits grow from cell enlargement, not cell division?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: How am I doing? [Re: FriedEgg]
#22434702 - 10/26/15 05:07 AM (8 years, 3 months ago) |
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They grow from both. Some species like hericium grow almost exclusively from division. Far as I can tell cubes start out mostly growing from division and water expansion kicks in as it develops. Many think that once the veil tears the growth at that point is almost all water weight. But certainly division is occurring past the point of pin formation.
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rudeboi
Noob

Registered: 05/17/14
Posts: 107
Loc: North BC
Last seen: 5 months, 13 days
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Pastywhyte said: They grow from both. Some species like hericium grow almost exclusively from division. Far as I can tell cubes start out mostly growing from division and water expansion kicks in as it develops. Many think that once the veil tears the growth at that point is almost all water weight. But certainly division is occurring past the point of pin formation.
I share this view.
I'd say it goes from like 90% division and 10% expansion as a pin to 90% expansion and 10% division right as the veil is tearing. That's a guess of course, based on observation and time scale. I'd say producing spores also takes a little something out of the mycelium's vigor for whatever reason.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: Inocuole]
#22435179 - 10/26/15 09:19 AM (8 years, 3 months ago) |
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Okay so I have my plates ready. Just to confirm, can I pick a pin and just throw it on my plate or do I have to take it into my SAB, split it with a sterile blade and extract tissue?
If I do just throw it on the plate, do I wipe it down with alcohol or anything or will the mycelium just take over contaminants? What's the largest pin I want to work with? When I get home today I'll take a new pic and maybe you guys can hell me decide which pins and fruits to clone. These tubs are ridiculous. I might just get a canopy on my first try!!
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How am I doing? [Re: Kalistis]
#22435195 - 10/26/15 09:23 AM (8 years, 3 months ago) |
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Okay so....
What does "throwing a pin on a plate" mean to you? Where is the plate while this is happening? You only mentioned the SAB in the second option.
Either works but.. not if your first option means you're opening an agar plate in open air, obviously. Another obvious being that the inside of a pin is cleaner than the outside. So, take the risk if you want. Whole pins are more likely to contam than a piece of the inner tissue, but if it's real small then that's what it is.
Don't put iso on the pin, but do wipe the outside of the plates down with it while you're working.
I recommend the biopsy option but.. it's a matter of preference.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: Inocuole]
#22435479 - 10/26/15 10:52 AM (8 years, 3 months ago) |
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Oh, I intended on using my SAB in either scenario. I was just confused if I should biopsy the pin or just literally put it on the plate. Maybe I'll try both? I poured 50 plates last night as I'm expecting a shipment as well sometime this week. Yay.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 10 days
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Re: How am I doing? [Re: Kalistis] 1
#22435482 - 10/26/15 10:54 AM (8 years, 3 months ago) |
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Quote:
50 plates
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Damn, how'd I miss this one. Good job!
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: How am I doing? [Re: Kalistis]
#22436943 - 10/26/15 05:44 PM (8 years, 3 months ago) |
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Quote:
Kalistis said: Oh, I intended on using my SAB in either scenario. I was just confused if I should biopsy the pin or just literally put it on the plate. Maybe I'll try both? I poured 50 plates last night as I'm expecting a shipment as well sometime this week. Yay.
either one will work. my personal preference is to split the stem with my fingers and take a biopsy from the center.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: FriedEgg] 1
#22437579 - 10/26/15 08:34 PM (8 years, 3 months ago) |
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I have a dilemma. Most of what I have read about uneven substrate and pinning says to just pick the fruits as they mature, but my fruits are so closely clustered and at such different levels of maturation that the task isn't as simple as it seems. I will surely damage the smaller pins on the outer most edge during this process.
I see that I have 3 options here:
1. Should I just pick most of the large clusters to avoid the near fully mature guys from dropping spores?
2. Do I wait and let the most mature guys fully mature and drop what they will, allowing for the smaller fellows to catch up?
3. Do I selectively and systematically pick those closest to maturity and leave the little guys giving them a chance to catch up over the next 24 hours and pick as they come?
Lastly, are these darker guys going to abort? They are still growing but look funny.

Thanks again guys.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How am I doing? [Re: Kalistis]
#22437587 - 10/26/15 08:35 PM (8 years, 3 months ago) |
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I'd just go ahead and do a clean sweep and get it all if it were me. You can do any of the things you said though, it's all up to you.
Not sure if they're aborts, really won't matter much if you pick them in the next 24 hours.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: Inocuole]
#22437659 - 10/26/15 08:57 PM (8 years, 3 months ago) |
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Clean sweep all out, even the little tiny pins on the perimeter or leave those for next flush? What do I do with those little tiny guys then? Dry them? I have been popping aborts here and there. Fresh mushrooms are delicious, BTW. I get a little head spacey but def. don't trip. I was considering eating some tomorrow on my day off.
I was planning on adding a couple of cups of water to the substrate once I was done instead of doing a full dunk. What do you think?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: How am I doing? [Re: Kalistis]
#22437675 - 10/26/15 08:59 PM (8 years, 3 months ago) |
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You can leave the really little pins. If something is part of a cluster and it's about to drop spores, I just take the whole cluster, though.
It doesn't hurt yield too bad though to just grab it all. Harvesting large amounts off a tub seems to delay the maturation of the other fruits.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: Inocuole]
#22438447 - 10/27/15 12:18 AM (8 years, 3 months ago) |
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I made 8 clones prior to harvest of 1 out of the 2 GT tubs. I don't have high expectations there.... I most certainly feel like I had to have contaminated the plates during that process.
As far as the harvest, 420 grams of fruit from the partial flush out of one tub. Since none of the clusters on the sides looked ready to go, I didn't touch them. I figure I can harvest them tomorrow with the other tub. The first batch is in my dehydrator now. 
Is it normal for there to be a full pin set ready to go just below all of these fruit or are those just going to abort?



Hopefully these will be cracker dry by morning so I can get another batch in. The manual says to do mushrooms at 130 for 2 to 4 hours if they are sliced. Based on what I have read, I decided to bump it up to 160 (machine goes up to 165, but I decided not press my luck) for 4 hours. I will heck them then and add time if necessary.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: How am I doing? [Re: Kalistis]
#22441616 - 10/27/15 07:09 PM (8 years, 3 months ago) |
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good job
i put mine in the dehydrator for 12-14 hours. sometimes longer if needed. it wont hurt.
-------------------- (Yes, the egg is real)
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: FriedEgg]
#22447263 - 10/28/15 11:30 PM (8 years, 3 months ago) |
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UPDATE: Here's what they look like this evening. A little bruised, but I am surprised that they haven't taken any time to recover. Even the crack in the substrate in the one tub has a web of mycelium now. I think the number of pins is crazy. The edges blew up overnight. I wonder if the middle is going to abort like mad? I see that some of the pins that were near larger fruits have bruised. They aren't soft yet and I worry about doing more damage to the network if I pick at it. The first flush had some pretty small fruit. These seem to be growing taller.



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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: How am I doing? [Re: FriedEgg]
#22447267 - 10/28/15 11:31 PM (8 years, 3 months ago) |
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Quote:
FriedEgg said:
Quote:
Kalistis said: Oh, I intended on using my SAB in either scenario. I was just confused if I should biopsy the pin or just literally put it on the plate. Maybe I'll try both? I poured 50 plates last night as I'm expecting a shipment as well sometime this week. Yay.
either one will work. my personal preference is to split the stem with my fingers and take a biopsy from the center.
I followed this advice and it seems to be working like a charm. Thanks!
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