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OfflineRohypnol
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Registered: 08/04/03
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Treating pressure cooked susbtrate with Actinomyces?
    #2242735 - 01/14/04 10:40 PM (13 years, 4 months ago)

Actinomyces are the thermophilic bacteria that colonize substrate after pasturizing it, and prevent other bacteria from getting a foot-hold. Mushroom mycellium can easily eat this bacteria, and break it down to beneficial nutrients. I was wondering if it would be practical to ensure substrate success by treating it with Actinomyces after pressure cooking it.

What Actinomyce in particular would be good for this purpose? I suppose the one with the fastest fission cycles, and the easiest digestability would be the ideal choice.


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Invisiblemicro
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Re: Treating pressure cooked susbtrate with Actinomyces? [Re: Rohypnol]
    #2243453 - 01/15/04 09:15 AM (13 years, 4 months ago)

Quote:

Actinomyces are the thermophilic bacteria that colonize substrate after pasturizing it



Not pasteurized substrate, like straw -- these are thermophiles, like you said, and grow at high temperatures. They're only really present in composting.
Quote:

I was wondering if it would be practical to ensure substrate success by treating it with Actinomyces after pressure cooking it.



No -- with sterile culture you want sterile culture -- bulk is spawned with a lot of mycelium, which is different than a few (thousand) spores/hyphal cells/whatever. Besides, you aren't growing the actinos at 150F, so there wouldn't be much of a point.
Quote:

What Actinomyce in particular would be good for this purpose?



A thermophile. There are a lot of them....

--
Micro


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Invisiblemycofile
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Re: Treating pressure cooked susbtrate with Actinomyces? [Re: micro]
    #2244266 - 01/15/04 04:30 PM (13 years, 4 months ago)

I agree that it's not practical for sterile substrates.

But, if you want to use them on bulk substrates, casing mixes etc, just hold the temp at around 120*F for a day or three after pasteurizing. They are abundant in nearly anything you would be using, from peat to straw to whatever, so you won't need to purchase/culture/add a specific species. If you want to be sure, just throw a handful of soil or aged manure in as a starter culture. The bob harris express composter is ideal for this treatment.

BTW if, like most who have tried it, you have trouble growing pans on pasteurized straw alone (no dung), culturing the acti's on the pasteurized straw by the method above will improve your results and lower your contams drastically.


--------------------
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Invisiblesci33
AmbitiousScientist
Registered: 11/11/03
Posts: 186
Re: Treating pressure cooked susbtrate with Actinomyces? [Re: mycofile]
    #2244766 - 01/15/04 07:47 PM (13 years, 4 months ago)

Quote:

mycofile said:
just hold the temp at around 120*F for a day or three after pasteurizing.




Aside from using an oven, how would you hold the temperature at around 120F for a whole day?


--------------------
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Invisiblemycofile
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Re: Treating pressure cooked susbtrate with Actinomyces? [Re: sci33]
    #2246969 - 01/16/04 08:59 PM (13 years, 4 months ago)

Quote:

  mycofile said:
The bob harris express composter is ideal for this treatment.



:wink:
I love being a smart ass!
But seriously, the Bob Harris express composter is basically a steam humidifier wired with a dimmer switch used to steam a styrofoam coller full of substrate.  Most people actually buid something larger, which the hummers can't heat enough, so they use alternate steam sources (wallpaper steamers, steam cleaners etc)
You should be able to find the plans and write up with a quick search, it's been posted many times over the years, with several people trying to take credit for it.  It's an easy build, and is great for making compost very quickly (which pans love btw) as well as steam pasteurization etc.  I'll find some links if you can't, lemmeknow.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


Edited by mycofile (01/16/04 08:59 PM)


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Invisiblesci33
AmbitiousScientist
Registered: 11/11/03
Posts: 186
Re: Treating pressure cooked susbtrate with Actinomyces? [Re: mycofile]
    #2259851 - 01/21/04 03:02 PM (13 years, 4 months ago)

Thanks! Time to begin searching.. oh the possibilities once I make this..


--------------------
Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)

Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)

PM for a trade.


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Invisiblesci33
AmbitiousScientist
Registered: 11/11/03
Posts: 186
Re: Treating pressure cooked susbtrate with Actinomyces? [Re: mycofile]
    #2259950 - 01/21/04 03:35 PM (13 years, 4 months ago)

Myco, I found a couple of stuff but most of the links were outdated, this is the only viable link I found:

http://www.shroomery.org/forums/showflat...amp;o=&vc=1

Is that what you are talking about or are there better plans out there? Thanks.


--------------------
Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)

Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)

PM for a trade.


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Invisiblemycofile
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
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Re: Treating pressure cooked susbtrate with Actinomyces? [Re: sci33]
    #2262742 - 01/22/04 02:23 PM (13 years, 4 months ago)

That's the one, should also be in the grow section as a hard document. Try searching in advanced for "wall paper steamer" and you should see some posts of larger units than just a styro cooler. Those little hummers just can't heat up larger containers.

BTW, wagner has an outlet section of their site and you can get a hell of a steam generator in the $30 range.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Invisiblemycofile
Pooh-Bah
 User Gallery

Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Treating pressure cooked susbtrate with Actinomyces? [Re: mycofile]
    #2262765 - 01/22/04 02:31 PM (13 years, 4 months ago)

Click Here For a particularly useful thread I remember.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Invisiblesci33
AmbitiousScientist
Registered: 11/11/03
Posts: 186
Re: Treating pressure cooked susbtrate with Actinomyces? [Re: mycofile]
    #2265772 - 01/23/04 02:43 PM (13 years, 3 months ago)

Thanks.. time to read.


--------------------
Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)

Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)

PM for a trade.


Post Extras: Print Post  Remind Me! Notify Moderator
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