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Offlinemushpunx
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Pinpointing contams, agar to grain
    #22426330 - 10/24/15 09:16 AM (8 years, 3 months ago)



Hey all

    So I had a rough summer as far as contams go. Despite many hours of reading and as much improvemt in most every area as Ive learned how to make Im still losing a few  tubs out of every round. Granted I spawn a lot of tubs but its certainly not acceptable.

  So I grew out some new MS cultures, the photos aren't too great but they are absolutely clean to the eye.
Im very confident of my culture work actually, I never see so much as a spot of bacteria or a colony.

Which makes me think its not my flowhood, I would think if it was it would show up in my plates at some point? If I can transfer from plate to plate with no problems then I doubt innoculating my master jars is the issue. Im thinking its happening during G2G somewhere.

So Im going to inoculate 12 jars tonight, that I sterilized at 25 PSI for 90 minutes, with 3 very clean plates. Im going to spawn these to 3 tubs, pasteurized coir. Pretty safe. I mean agar to grain to pasteurized coir... if any of these contam Im gunna be quite confused.

Originally I was thinking I should knock them up in my SAB, then if they go fine Ill do the same thing in front of my hood and see if there's a difference.

  Sound like a plan?


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