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OfflineMysticMoteToter
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Should spores be visible when inocing' agar?
    #22424439 - 10/23/15 07:44 PM (8 years, 3 months ago)

Hey everyone thanks for looking. Anyways yesterday it was my first try at agar work and now i'm just waiting to see if my spores are going to germinate. The print i used was very, very light. When i was doing the agar inoc. i wasn't able to get many spores (visible to the naked eye), Only 2 out of my 5 plates have spores visible to the naked eye. Will this be okay, I've heard that even a very light print is viable but do i need to get enough spores on agar to be visible with the naked eye or should just swiping the print then agar without visible spores sufficient to germinate spores? Regardless i guess i'll have my answer in a few days but If i'm supposed to have a visible amount of spores on agar i'm going to go back and re-inoc. the 3 plates where i can't see spores. I appreciate the feedback, thanks everyone.


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Invisibletahoe
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Re: Should spores be visible when inocing' agar? [Re: MysticMoteToter]
    #22424451 - 10/23/15 07:47 PM (8 years, 3 months ago)

You don't need to be able to see them.


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OfflineMysticMoteToter
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Re: Should spores be visible when inocing' agar? [Re: tahoe]
    #22424514 - 10/23/15 08:03 PM (8 years, 3 months ago)

Thank you tahoe!


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Invisiblebootster
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Re: Should spores be visible when inocing' agar? *DELETED* [Re: MysticMoteToter]
    #22425234 - 10/23/15 11:06 PM (8 years, 3 months ago)

Post deleted by bootster

Reason for deletion: Not feeding the trolls.



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InvisibleInocuole
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Re: Should spores be visible when inocing' agar? [Re: bootster]
    #22425239 - 10/23/15 11:08 PM (8 years, 3 months ago)

Quote:

bootster said:
hurts your consolidation.




Can you.. show me on the doll, where that is?


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OfflinecronicrFacebook
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Re: Should spores be visible when inocing' agar? [Re: Inocuole]
    #22425515 - 10/24/15 12:55 AM (8 years, 3 months ago)

:lolsy:


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OfflineMysticMoteToter
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Re: Should spores be visible when inocing' agar? [Re: Inocuole]
    #22427249 - 10/24/15 01:16 PM (8 years, 3 months ago)

Quote:

Inocuole said:
Quote:

bootster said:
hurts your consolidation.




Can you.. show me on the doll, where that is?



We're going to need three dolls :creepylurker:


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InvisibleInocuole
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Re: Should spores be visible when inocing' agar? [Re: MysticMoteToter]
    #22427254 - 10/24/15 01:17 PM (8 years, 3 months ago)

Quote:

MysticMoteToter said:
We're going to need three dolls :creepylurker:



That's a case I wouldn't want to pick up as a social worker..


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Invisiblebootster
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Re: Should spores be visible when inocing' agar? [Re: MysticMoteToter]
    #22454544 - 10/30/15 05:30 PM (8 years, 2 months ago)

https://www.shroomery.org/forums/showflat.php/Number/14258735

Sorry if my term wasn't "definitive" enough for you. An adult response would have been a MINOR correction. I see that we are lacking a few.

I believe that the poster deserves a response that helps him. Apparently that isn't so with you guys..


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OfflinecronicrFacebook
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Re: Should spores be visible when inocing' agar? [Re: tahoe]
    #22454569 - 10/30/15 05:34 PM (8 years, 2 months ago)

Quote:

bootster said:

I believe that the poster deserves a response that helps him. Apparently that isn't so with you guys..



Quote:

tahoe said:
You don't need to be able to see them.



very first answer


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It doesn't matter what i think of you...all that matters is clean spawn

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InvisibleLocN9ne
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Re: Should spores be visible when inocing' agar? [Re: cronicr]
    #22454619 - 10/30/15 05:47 PM (8 years, 2 months ago)



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OfflineMysticMoteToter
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Re: Should spores be visible when inocing' agar? [Re: LocN9ne]
    #22454726 - 10/30/15 06:10 PM (8 years, 2 months ago)

While you're all here.... haha, should i continue to let this grow out or transfer the heavier growth in the middle in the middle to a new plate? This is after 8 days growth.These are the "fastest" growing of the 5 plates i made (petris #1 and #5). #1 has a decent amount of condensation on the lid so i took pics from below too.


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OfflinecronicrFacebook
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Re: Should spores be visible when inocing' agar? [Re: MysticMoteToter]
    #22454741 - 10/30/15 06:14 PM (8 years, 2 months ago)

never transfer from the center, use the leading edge it grows faster from there


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

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OfflineMysticMoteToter
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Re: Should spores be visible when inocing' agar? [Re: cronicr]
    #22454782 - 10/30/15 06:27 PM (8 years, 2 months ago)

thanks cron, then ill have to wait for it to bulk up a bit! thanks guys


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OfflinecronicrFacebook
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Re: Should spores be visible when inocing' agar? [Re: MysticMoteToter]
    #22454786 - 10/30/15 06:27 PM (8 years, 2 months ago)

rigth on man look foreward to your updates


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OfflineDrCrumbs
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Re: Should spores be visible when inocing' agar? [Re: cronicr]
    #22455078 - 10/30/15 07:33 PM (8 years, 2 months ago)

your choking em with scotch tape


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Offlinemushpunx
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Re: Should spores be visible when inocing' agar? [Re: DrCrumbs]
    #22455293 - 10/30/15 08:11 PM (8 years, 2 months ago)

Quote:

DrCrumbs said:
your choking em with scotch tape





Is that tape? If you don't have parafilm, cut a 1.5 to 2" section off a roll of cling wrap and wrap with that :thumbup:


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InvisibleLocN9ne
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Re: Should spores be visible when inocing' agar? [Re: mushpunx]
    #22455307 - 10/30/15 08:15 PM (8 years, 2 months ago)

Looks like cling wrap to me.


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Offlinemushpunx
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Re: Should spores be visible when inocing' agar? [Re: LocN9ne]
    #22455371 - 10/30/15 08:28 PM (8 years, 2 months ago)

Quote:

LocN9ne said:
Looks like cling wrap to me.




Could be :shrug: . Still the wrap job leaves something to be desired haha. It takes a little practice, hell get there. Should stretch the wrap, wrap it a lot tighter :thumbup:


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OfflineMysticMoteToter
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Re: Should spores be visible when inocing' agar? [Re: LocN9ne]
    #22455404 - 10/30/15 08:37 PM (8 years, 2 months ago)

Quote:

LocN9ne said:
Looks like cling wrap to me.



It's press n seal if thats the same thing, but it seems to be working. its just what i had laying around  :shrug2:

edit: it's my first attempt at growing so im just happy i was able to get 8 days on agar w/o contams. but it took about 6 days before any actual tomatose myc showed up. At first i though it was bacterial but when i put it under a light i could see little branch like growth and thought its was probably myc and let em go. 2 days ago they finally started getting cottony but around them theres still the transparent myc growth is this normal? It's very white i dont think it's contams and it's on MEA i got from a sponsor so the agar is good. Here are pictures to show you what i was talking about. Could it be lacking FAE due to the cling wrap or is this how normal cube myc. looks on agar just beginning germination? It's becoming tomatose (i probably spelled that wrong haha) now so its good but its working its way over the transparent myc very it seems.

^same plate just two days ago. It first showed up about 3-4 days after i inoced the plates.
Thanks again for the responses everyone. I appreciate the knowledge and help.


Edited by MysticMoteToter (10/30/15 08:50 PM)


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InvisibleLocN9ne
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Re: Should spores be visible when inocing' agar? [Re: mushpunx]
    #22455436 - 10/30/15 08:43 PM (8 years, 2 months ago)

Like mush said...
Quote:

mushpunx said:
Quote:

LocN9ne said:
Looks like cling wrap to me.




Could be :shrug: . Still the wrap job leaves something to be desired haha. It takes a little practice, hell get there. Should stretch the wrap, wrap it a lot tighter :thumbup:




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InvisibleInocuole
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Re: Should spores be visible when inocing' agar? [Re: bootster]
    #22455483 - 10/30/15 08:58 PM (8 years, 2 months ago)

Quote:

bootster said:
Post deleted by bootster<p>Reason for deletion: Not feeding the trolls.



Uh huh, kay.  I guess anyone who doesn't understand what you're trying to say could be a troll.  What does consolidation have to do with agar again?  I can't tell since you deleted your post.

Quote:

bootster said:
https://www.shroomery.org/forums/showflat.php/Number/14258735

Sorry if my term wasn't "definitive" enough for you. An adult response would have been a MINOR correction. I see that we are lacking a few.

I believe that the poster deserves a response that helps him. Apparently that isn't so with you guys..





Oh but wait, just before that you said you weren't gonna feed me.... Just up there.... now I feel lied to.


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Offlinemushpunx
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Re: Should spores be visible when inocing' agar? [Re: MysticMoteToter]
    #22455517 - 10/30/15 09:03 PM (8 years, 2 months ago)

Quote:

MysticMoteToter said:
Quote:

LocN9ne said:
Looks like cling wrap to me.



It's press n seal if thats the same thing, but it seems to be working. its just what i had laying around  :shrug2:

edit: it's my first attempt at growing so im just happy i was able to get 8 days on agar w/o contams. but it took about 6 days before any actual tomatose myc showed up. At first i though it was bacterial but when i put it under a light i could see little branch like growth and thought its was probably myc and let em go. 2 days ago they finally started getting cottony but around them theres still the transparent myc growth is this normal? It's very white i dont think it's contams and it's on MEA i got from a sponsor so the agar is good. Here are pictures to show you what i was talking about. Could it be lacking FAE due to the cling wrap or is this how normal cube myc. looks on agar just beginning germination? It's becoming tomatose (i probably spelled that wrong haha) now so its good but its working its way over the transparent myc very it seems.

^same plate just two days ago. It first showed up about 3-4 days after i inoced the plates.
Thanks again for the responses everyone. I appreciate the knowledge and help.




Dont worry so much about fluffy or rhizo growth, it actually doesnt speak much to how good the culture is. A fluffy culture will go rhizo if you lower the nutrition content of the agar and vice versa.
That being said just look for strong, healthy growth. Take from the leading edge, the farthest part of healthy growth from the center of the culture.

Germination plates usually look funky. Just try to take a piece that's clean from it. Id take a transfer from that right now, I usually do when the germination is about the size of a dime or so.. you wanna transfer from germination plates as soon as you can get a clean piece... before it grows over any mold or bacteria.

Its not FAE, usually you wrap the plate a bit tighter than that. You're doing fine man! Take a transfer from that plate, on the first plate it doesnt really matter where from just take something clean.


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OfflineMysticMoteToter
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Re: Should spores be visible when inocing' agar? [Re: mushpunx]
    #22476708 - 11/04/15 02:10 PM (8 years, 2 months ago)

So i made some transfers on Halloween, i thought #5 was going to be the best but the #1 plate really took off. I kept all my plates aside from one of the initial that didn't germinate and one of the transfers i had to toss due to some weird satellite colonies. The first plate i inoced is growing extremely strong and the transfer from it looks good so far as well. I was wondering if i could use the myc from the initial plate to make a myc water slurry and do PF. My main concern was ensuring a clean growth vs isolating a strain b/c i got the print for a spore ring but i wanted to get some opinions before i use the myc from #1 to make a LI to inoc. my cakes.

Could i use the first plate i inoced to do a LI or should i wait for the new transfer to grow out to have more of an isolated culture? Thanks everyone


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OfflineMysticMoteToter
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Re: Should spores be visible when inocing' agar? [Re: MysticMoteToter]
    #22476740 - 11/04/15 02:20 PM (8 years, 2 months ago)

Pics of the plates head on i'm thinking of using

The 2nd pic looks like it has some sort of other colony in there with it buts thats the #2 petri visible through #1 which i'm holding up. Sorry about that if it confuses anyone for a bit haha


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