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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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agarless tissue plant
#22419769 - 10/22/15 07:17 PM (8 years, 3 months ago) |
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I bought a likely looking shiitake mushroom today from my local supermarket--they had a bin of loose, rather small but shapely shiitakes on display. I think the price was something like 4.99 @lb. I took my smushroom home, fired up my alki lamp, scorched my tweezers--I have a wonderful set of big tweezers (Harbor Freight)--and set to work. I snipped off the cap and began to tear the stipe down the middle with my bare hands. Then I put the half-ripped stipe in the SAB, where the tweezers were waiting and cooling and went to get an agar jar.
Here is where the plot thickens:I could find no prepared pasty dishes. All I had was a freshly--yesterday--sterilized 4 oz jar of wbs that I was going to use for something else (which I won't go into just now).
Well, to shorten this report to a merciful length, I went full bore ahead anyway, charging like a rhino, grabbed the jar of wbs, put it in the SAB and got to work. Taking up the sterile tweezers I seized the stipe, finished tearing it lengthwise in two,took a third pair of tweezers, fired it, used it to pull a bit of tissue from the freshly exposed innards of the stipe and dropped that bit of tissue into the little jar of wbs.
Question: Has anyone ever tried anything like this? or heard of it being tried? Is success anywhere within a 100 miles of my grasp?
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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dreadhed

Registered: 01/21/11
Posts: 175
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Re: agarless tissue plant [Re: champinhom]
#22420031 - 10/22/15 08:10 PM (8 years, 3 months ago) |
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I've always put clones to agar first. I've taken clean petri dishes on the first try with clones. So I'd say it's possible & your within at least 90 miles of success. Good luck!
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: agarless tissue plant [Re: dreadhed]
#22420105 - 10/22/15 08:24 PM (8 years, 3 months ago) |
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Quote:
dreadhed said: I've always put clones to agar first. I've taken clean petri dishes on the first try with clones. So I'd say it's possible & your within at least 90 miles of success. Good luck!
Yes, I'd say I have about 1 chance in 10. If the tissue is clean and if it will sprout mycelium and if the mycelium can get a grip on the surface of the wbs...
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 3 months, 19 days
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Re: agarless tissue plant [Re: champinhom]
#22421172 - 10/23/15 01:00 AM (8 years, 3 months ago) |
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Works with oysters, sometimes you don't even need to sterilize anything, just drop a piece of the oyster stem in and go, but that it because oysters are fairly vigorous. Not sure how it will pan out with your Shitake.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: agarless tissue plant [Re: Mrcloudy]
#22425598 - 10/24/15 01:31 AM (8 years, 3 months ago) |
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Quote:
Mrcloudy said: Works with oysters, sometimes you don't even need to sterilize anything, just drop a piece of the oyster stem in and go, but that it because oysters are fairly vigorous. Not sure how it will pan out with your Shitake.
I will post any further developments on this thread.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: agarless tissue plant [Re: champinhom]
#22426836 - 10/24/15 11:40 AM (8 years, 3 months ago) |
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so now it is day two of the agarless experiment. The bit of shiitake tissue is sitting on top of the WBS with no sign of having done anything yet. If I had to assign a mood to this bit of tissue, I would say it is: Serene. Another thing I might say about it is that it is white as the driven snow and looks quite viable.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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