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OfflineIfishhigh
Stranger

Registered: 03/21/15
Posts: 570
Last seen: 4 years, 6 months
Spore syringes?
    #22419386 - 10/22/15 05:59 PM (8 years, 3 months ago)

Ok I'm a newb, and I have to ask some dumb questions
I have got my syringes in today and I can't start my grow yet.

1. I opened box to look at them and two of the three had lots of black particles and the third was clear. I guess from my research this is nothing to worry about. My question is I just put them back in the box and placed in the refrigerator. I don't have any ziplock bags. Is this a bad storage method ?
Until tomorrow I have no choice.

2. Leaving the syringes in the fridge with no ziplock got me thinking. I'm suppose to use a glove box,gloves,tyvec sleeves,sterilized jars and substrate, and flame the needle. Only thing is the syringe itself will not be sterile. Am I missing something? Or is it one of those things where, it is what it is. Everything will be sterile except for the syringe correct ?

Again im a newb and want to do everything right and I just have so many thought going through my head. O which brings me to one last question

3. I have denatured alcohol. I seen a tek on here on how make a lamp with a half pint glass. But in the last pic it shows the flame burning but the flame looks orange. Shouldn't the flame be blue if it's working properly? does anyone just use a shot glass ? I can't find a lamp and don't want to order one either. Unless of chours you recommend to.

Any Answere to my questions will be appreciated. And any recommendations for the lamp issue would be cool also. Thanks guys


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InvisibleStickyIcky Fingers
Free DOOM!! Available Here
Registered: 12/03/13
Posts: 178
Re: Spore syringes? [Re: Ifishhigh]
    #22419475 - 10/22/15 06:15 PM (8 years, 3 months ago)

they may take a little longer to germinate if stored in the fridge for consecutive days. that should be fine just leave the caps on the syringes

The inside of the syringe will have spores in it so it is not sterile but it is hopefully clean: only having the spores of the desired species present in the syringe. Syringes (and other medical supplies) are sterilized by the manufactures and syringes can be repeatedly sterilized with boiling water or better a PC run when you want to reuse them.

It may be just the colors that the camera picked up.... I dont know I dont see any problems it'll be fire either way.

I dont know about shot glasses or alc lamps I use a bic lighter or a propane dab torch


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OfflineIfishhigh
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Registered: 03/21/15
Posts: 570
Last seen: 4 years, 6 months
Re: Spore syringes? [Re: StickyIcky Fingers]
    #22419514 - 10/22/15 06:24 PM (8 years, 3 months ago)

I was referring to the plastic surface of the syringe that is not sterile. Should I wipe it down with some alcohol?


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Offlineorison
mcfluffysugarnuts


Registered: 01/19/09
Posts: 5,468
Last seen: 24 days, 15 hours
Re: Spore syringes? [Re: Ifishhigh]
    #22419543 - 10/22/15 06:31 PM (8 years, 3 months ago)

shake the clumpy syringes like a Polaroid picture before use..


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OfflineMachiavelliavore
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Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
Re: Spore syringes? [Re: Ifishhigh]
    #22419545 - 10/22/15 06:32 PM (8 years, 3 months ago)

They should be fine in the fridge.  I forget if it's standard to store them in the fridge or at room temp.  Plunger on one side, needle that will have its contents burned before use.


Nothing in a still air box is sterile.  Only what's inside vessels that went through the pressure cooker/steaming will be sterile.  If you're going to take the syringe into the airbox, wiping it down with alcohol to try and avoid flying particles is a decent precaution.  No need to get OCD about it.  If your injecting through ports or into PF cakes, it really won't matter much.  If your opening jars, do not put the syringe or your hands above the open jar, only the needle.

Inoculating through injection ports/poly stuffing/PF cakes does not require an airbox.  Only opening containers requires that.  It can't hurt though.  Leave foil coverings over ports/poly/PF until you are ready to inject.  That way when the foil comes off it's pretty clean and you won't be taking much with the needle as it goes in.  Turn of AC/Fans/Heating and shut doors and windows when inoculating to minimize what will be falling ontop of your work.


I think photos tend not to pick up the blue flame very well.  Probably can't see a blue section that's there.  I know nothing about this topic, I just use a shitty bic.

Black particles are spore flakes.  Sometimes there are not enough spores to be obvious to the naked eye, though that is absolutely still plenty for it to do its job fine.  Albinos have translucent spores, and RedBoy/PF Redspore/Cambodian Rustspore have offcolour red/rusty spores.  Else dark purple-brown.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleStickyIcky Fingers
Free DOOM!! Available Here
Registered: 12/03/13
Posts: 178
Re: Spore syringes? [Re: Ifishhigh]
    #22419558 - 10/22/15 06:34 PM (8 years, 3 months ago)

if you want to but the side of the syringe shouldn't come in to contact with the substrate its possible that something/endospores could fall off the syringe but I think it would be more detrimental to have the syringe covered in alcohol before flame sterilizing the needle


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OfflineDrCrumbs
Alpha Blue


Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
Last seen: 4 years, 2 months
Re: Spore syringes? [Re: StickyIcky Fingers]
    #22419631 - 10/22/15 06:49 PM (8 years, 3 months ago)

They were just loose in the box?

Seems like they would have already been in a bag.

Use a lighter, dont try to make a lamp.

Can always clean everything with iso.

fridges can be pretty dirty. However a clean needle, is a clean needle. It wont effect the inside of the syringe.

Don't touch dirty surfaces and then surfaces that should be/remain clean.


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