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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter] * 1
    #22420060 - 10/22/15 08:15 PM (8 years, 3 months ago)

Quote:

MysticMoteToter said:
PCing some agar right now wish me luck everyone and thank you all for the info on here!
:offthehook:





Congrats man. Dont forget pics of harvest. Or it didn't happen :p


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OfflineMysticMoteToter
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Re: So stoked, finally got the spores to start my 1st grow! [Re: spacechildo]
    #22420110 - 10/22/15 08:24 PM (8 years, 3 months ago)

I innoced them and wrapped them in glad press n seal (its all i had), some of the dishes the condensation disappeared but a few still have heavy condensation on the lid. Also where is the best place to keep them from getting contaminated? Can i just keep them in the SAB or is there anywhere better? Thanks again everyone


--------------------

:zaphod:   :zaphod: 


Half Homo Hardly Sapient
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Fuck War, Feed Birds.



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OfflineMachiavelliavore
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Re: So stoked, finally got the spores to start my 1st grow! [Re: spacechildo]
    #22420144 - 10/22/15 08:32 PM (8 years, 3 months ago)

You can store the plates anywhere.  You're probably gonna want to look at them a lot so put'm somewhere convenient.  Why didn't you just put the original lids back on the plates?  Anyway, condensation isn't a huge deal, as long as there isn't a big puddle sloshing around in the plate.  Good luck.


I like to have paper towels in the bottom of my box well soaked with 1:10 bleach.  I actually think soapy water is a better idea for preventing contams from lifting off, but I started down this path and I just <3 to kill shit and I haven't had any problems with it.

Anyway, the paper towels help prevent vibration when contact is made with the floor of the air box, and help keep it uniformly wet, while plastic will be dry in some areas quite quickly, unless you're working in a puddle.

I like to spray down the tote, wipe it down, spray it down again.  Spray down the lid, put paper towels, spray down the towels, put in alcohol swabbed contents, place the tote on, then mist down again through the arm holes, and let it sit till work commenses.

#1 Rule:  Don't put your hands above your work.

Don't worry about X chance of contamination per second, MUST BE SO FAST.  Just be steady.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineMysticMoteToter
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Re: So stoked, finally got the spores to start my 1st grow! [Re: Machiavelliavore]
    #22420193 - 10/22/15 08:39 PM (8 years, 3 months ago)

Now that they're wrapped and innoced' should i still have gloves and tyvek sleeves on when handling the plates or is washing my hands with soap then alcohol or HO2 and then using bare hands okay?


--------------------

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Half Homo Hardly Sapient
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Fuck War, Feed Birds.



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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter]
    #22420829 - 10/22/15 10:57 PM (8 years, 3 months ago)

Check pm

Edit:

Should be ok to toucb but I still wash my hands cuz my parafilm still tears sometimes. Gloves are not really necessary I dont use them a lot even in sab. I just make sure my hands dont go over an open plate or jar. Tyvek sleeves are only used in sab imo. I suspect this is so when your arms rub against the edge of the holes you dont fill the box with dead skin cells.could also be to close the hole better but I read a close fitting hole will agitate the air in you sab more when you take your hands out so I dont really know what to think on that one


Edited by Supalemonhaze (10/22/15 11:06 PM)


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OfflineMysticMoteToter
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Re: So stoked, finally got the spores to start my 1st grow [Re: MysticMoteToter]
    #22431917 - 10/25/15 01:16 PM (8 years, 3 months ago)

Well Its about 3 days since i poured the agar and inoculated it and theres no contams and the condensation has reduced :thumbup: !  Good so far, or would contams from the pour and inoc. and wrap show up later on? Thanks guys


--------------------

:zaphod:   :zaphod: 


Half Homo Hardly Sapient
Overview Effect
Fuck War, Feed Birds.



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OfflineoKiHai
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Registered: 10/21/15
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Re: So stoked, finally got the spores to start my 1st grow [Re: MysticMoteToter]
    #22431921 - 10/25/15 01:17 PM (8 years, 3 months ago)

Great I can't wait to get to the same place!


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OfflineMysticMoteToter
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Re: So stoked, finally got the spores to start my 1st grow [Re: oKiHai]
    #22432764 - 10/25/15 05:00 PM (8 years, 3 months ago)

What's stopping you? haha get crafty! I thought i'd never be able to get spores but if theres a will theres a way.


--------------------

:zaphod:   :zaphod: 


Half Homo Hardly Sapient
Overview Effect
Fuck War, Feed Birds.



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OfflineDesigner Drugs
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Registered: 03/22/15
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Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter]
    #22432808 - 10/25/15 05:08 PM (8 years, 3 months ago)

I wish you luck my dude!

Lol and fun fact, your makeshift glove box is the exact same kinda sterilite or whatever as my SGFC :tongue:


--------------------


"For the more we go through the fire,
the more refined we become.”


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OfflineMysticMoteToter
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Registered: 07/26/15
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Re: So stoked, finally got the spores to start my 1st grow! [Re: Designer Drugs]
    #22432837 - 10/25/15 05:13 PM (8 years, 3 months ago)

Thanks man, I appreciate it. So far im happy theres no bacterial growth so im pretty sure the pour and spores were clean (hopefully im not speaking too soon haha) i just cant wait to see some myc. crawling along  :biggrin: I accidentally got the opaque white lid sterlite for my SGFC haha had to work out how to light it from the side but i worked it out haha, still i wish i wouldve used this as my SGFC so i can get more ambient daylight as well as the 6500k CFL im putting on the side.


--------------------

:zaphod:   :zaphod: 


Half Homo Hardly Sapient
Overview Effect
Fuck War, Feed Birds.



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OfflineMachiavelliavore
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Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter]
    #22434524 - 10/26/15 01:39 AM (8 years, 3 months ago)

Even if a bit of bacteria does show up, you can always just transfer away from it.  Even if your technique is a sloppy, it's probably not going to be a lot.

LoL, make your airbox with the lid on the bottom and the lid colour doesn't matter.  Add to that, the lid almost always has greater surface area than the bottom of the tote, and thus provides more workspace.  You also don't have to lower all your shit into the tote, then close it, just place it on the lid then lower the box.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineMysticMoteToter
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Re: So stoked, finally got the spores to start my 1st grow! [Re: Machiavelliavore]
    #22436295 - 10/26/15 02:37 PM (8 years, 3 months ago)

Would bacteria would show up before mycelium?

Edit: still no signs of growth of anything


Edited by MysticMoteToter (10/26/15 02:38 PM)


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OfflineMachiavelliavore
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Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter]
    #22436324 - 10/26/15 02:46 PM (8 years, 3 months ago)

7 days is probably the median germination time, so nothing to worry about.  After two weeks you might get suspicious, but I mean, if it's a purple brown spore print, it's probably gonna do the trick.  You know what you've got much more than with a syringe.

The bacteria I've had has always shown up as a small milky brown/caramel coloured dot, and takes maybe 5 days to show up?  I'm not sure, I haven't payed that much attn to the timing.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineMysticMoteToter
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Registered: 07/26/15
Posts: 2,036
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Re: So stoked, finally got the spores to start my 1st grow! *DELETED* [Re: Machiavelliavore]
    #22436346 - 10/26/15 02:52 PM (8 years, 3 months ago)

Post deleted by MysticMoteToter

Reason for deletion: .



--------------------

:zaphod:   :zaphod: 


Half Homo Hardly Sapient
Overview Effect
Fuck War, Feed Birds.



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OfflineMysticMoteToter
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Registered: 07/26/15
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Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter]
    #22444078 - 10/28/15 09:39 AM (8 years, 3 months ago)

So its just about 6 days since inoc. and there is some growth but it's very, VERY transparent and is only about the size of a dime at largest in one of the plates. It originated only from the spots on inoculated so i think it's mycelium because it's slow moving as all hell. It has little branch patterns and i assume looks like the very, very beginning of mycelium growth. I cant get any clear macro pictures because it is so faint but it has a "veinlike" looking growth pattern and is nearly transparent...Idk i hope its young or monokaryotic mycelium or something but we shall see. ill probably be posting pics


Edited by MysticMoteToter (10/28/15 10:14 AM)


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OfflineMysticMoteToter
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Registered: 07/26/15
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Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter]
    #22444189 - 10/28/15 10:10 AM (8 years, 3 months ago)

Pictures added to help see if i'm still on track. I'm pretty sure it isn't bacteria, b/c, although you cant really tell in the pic, it has a very "veinlike" or "branchlike" growth pattern. Hoping it's mycelium, fingers crossed  :learning: Thank you all for your help and input!




--------------------

:zaphod:   :zaphod: 


Half Homo Hardly Sapient
Overview Effect
Fuck War, Feed Birds.



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OfflineMachiavelliavore
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Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter]
    #22444818 - 10/28/15 01:11 PM (8 years, 3 months ago)

Wow, that's really thin looking growth.  I hope it's cube mycellium.  Quite unlikely it's monokaryotic, from everything I read about that you need like 100x/1000x dilution of a spore syringe to get that.

How did you inoculate the plates?  I would have thought you'd have just scraped some spores on the print and poured them into the plate, but it looks like you only got'm at one point.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineMysticMoteToter
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Registered: 07/26/15
Posts: 2,036
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Re: So stoked, finally got the spores to start my 1st grow! [Re: Machiavelliavore]
    #22444907 - 10/28/15 01:32 PM (8 years, 3 months ago)

The print was extremely light and i got it from a free spore place, print was purple though so idk. And i used a loop to inoculate the dishes. hopefully it turns out but i didn't even notice any growth until yesterday really so maybe it'll perk up enough for a transfer. I have 4 other plates too and those have the same kind of growth just less of it.


--------------------

:zaphod:   :zaphod: 


Half Homo Hardly Sapient
Overview Effect
Fuck War, Feed Birds.



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OfflineprofessorFATTYCAP
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Re: So stoked, finally got the spores to start my 1st grow! [Re: MysticMoteToter]
    #22448489 - 10/29/15 09:33 AM (8 years, 3 months ago)

It looks good 2 go bro. Its possible ur agar isnt nutrient rich enuff for good strong growth. Or its a poor strain .but the growth def appears to be myc not contam


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: So stoked, finally got the spores to start my 1st grow! [Re: professorFATTYCAP]
    #22448566 - 10/29/15 10:05 AM (8 years, 3 months ago)

Ya congrats man. Im just spawning my first mini mono tub myself.

Race ya to harvest? :p I got some 2nd transfers that might be isolated but they are still pretty young to know. Ill post them pics when they grow a lil to see if they are isolated or not. Doesn't seem like they are sectoeing yet so fingers crossed


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