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OfflineDeadPhan
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How does or doesnt polyfil as a filter work for you?
    #22418553 - 10/22/15 02:27 PM (8 years, 3 months ago)

Thought of making as a poll. Ive had 100 percent success many times. Im trying to narrow down my source of contams. Im pretty certain its my workspace. Even with a flowhood this summer working out of garages with drafts....  im only starting back up again. I usually do good in the colder weather. I wonder at times if my filter on flowhood has gone bad. But there were some sterilized uninnoculated jars that i hadnt got to in maybe just over a month because i was busy with other shit that, by the time i got too all had trich. Ive had jars stay good for as long with poly but also not dead of summer and also no in a dirty garage. Im just curious on others experience. How long might you have had a sterilized jar before innoc stay good. Have you ever had a sterilized jar with poly filter go bad before opening. I wana switch to sfd's but dont have the money too atm. Poly is just so convenient and cheap and readily available at any dollar store. Ive had plenty of success with it but im starting to question how good a filter it may be. Im sure its inferior to most but as long as one works quick enough maybe not much to worry about. Im gonna do another run with it and if things dont pan out switch to sfd and if i still get contams i can rule out the filter and that maybe its my flowhood. Which would break my heart. Lol. Just never was apealed to a sab or glove box. I love working in open air amd get shit done quick. Id proly just build another fH before i worked with sab or gb but slacking on funds atm and the hepa i got on evay for real cheap is no longer avail. It was a clearance sale of sorts so i got a 2x2 foot one for 80 bux. Anyhoo. Just curious who ises poly and how it works for u


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Offlinetripdawg420
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Re: How does or doesnt polyfil as a filter work for you? [Re: DeadPhan]
    #22418572 - 10/22/15 02:31 PM (8 years, 3 months ago)

out grow has free shipping on sfd i used poly lids for year its best to have it in there pretty tight :thumbup:


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OfflineMachiavelliavore
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Re: How does or doesnt polyfil as a filter work for you? [Re: DeadPhan]
    #22418784 - 10/22/15 03:19 PM (8 years, 3 months ago)

I wouldn't expect poly filters to fail.  Perhaps you have put a needle(esp hot?) through the same poly enough times to create a channel for contams?  I think pasty uses poly for his plate setup.

Go grab some postal tyvek and use that for comparison.  Supposedly it only stays good for one round, though I've used it for many more without a problem.  Just grab a few priority envelopes and trace out circle with your jar lids and cut'm out with scissors.

Open up an agar plate for a reasonable amount of time (60s?) in your flow hood without doing any shitty sterile procedure and see if anything grows.  Maybe repeat for 2-3 plates to get a better sense.

Also, you could place your jars sideways, so you don't have gravity working contams through your poly.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: How does or doesnt polyfil as a filter work for you? [Re: Machiavelliavore]
    #22418812 - 10/22/15 03:26 PM (8 years, 3 months ago)

Considering I've had blank pasty plates with poly stuffed in my open living room for a few weeks with absolutely no contams on any of them, I'd say it works great lol. Might be different with grains getting shaken and disturbing the filter tho....


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Edited by Mad Season (10/22/15 03:30 PM)


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Invisibleoontribe
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Re: How does or doesnt polyfil as a filter work for you? [Re: Mad Season]
    #22418903 - 10/22/15 03:48 PM (8 years, 3 months ago)

I havent tried anything but poly...but it works perfect so no reason to change it.


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Invisibleazur
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Re: How does or doesnt polyfil as a filter work for you? [Re: DeadPhan]
    #22423835 - 10/23/15 05:08 PM (8 years, 3 months ago)

Dude, this summer was a bitch for everyone. I actually switched from plastic lids with sfd back to metal lids with tyvek. Helped tremendously


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A cube is NOT a cube.

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OfflineLogicaL ChaosM
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Re: How does or doesnt polyfil as a filter work for you? [Re: DeadPhan]
    #22424009 - 10/23/15 05:49 PM (8 years, 3 months ago)

Ive had zero issues with poly. Just got to stuff it right (not too loose or tight).


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"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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