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DampMushroom


Registered: 12/01/14
Posts: 115
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FIRST SECTORS/ISOLATES? *DELETED*
#22418257 - 10/22/15 01:07 PM (8 years, 3 months ago) |
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Post deleted by DampMushroomReason for deletion: I've got to leave for a couple years. Hope the best for all of y'all.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 12 hours, 38 minutes
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DampMushroom


Registered: 12/01/14
Posts: 115
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Re: FIRST SECTORS/ISOLATES? *DELETED* [Re: tripdawg420]
#22423187 - 10/23/15 02:08 PM (8 years, 3 months ago) |
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Post deleted by DampMushroomReason for deletion: I've got to leave for a couple years. Hope the best for all of y'all.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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How might the constancy of the fruit clusters size be with each of these cultures? My goal is to isolate away from all the crap while taking as reasonably healthy of a wedge as possible in the glovebox. I want every cap to look the same and I also want fatter yielding genetics any advice?
Once a plate starts to show sectoring you want to take a transfer from each sector to a new plate. I take at least a dozen. Then you would want to keep transfering from each of those dozen plates untill each of those no longer shows sectoring. These are your isolates.
Then you need to label each isolate very well and fruit each of them out, taking note of yeild and whatever other traits you are looking for, and most importantly potency. I still have a huge bag of weak mushrooms from a bomb clone... huge awesome yeild but weak ass potency.
You would then select the winning isolates to slant.
When you are working with a clone like you are its pretty much the same deal but less work. You'd wanna take your clone and fruit it out, check yeild and potency etc.. if its a keeper slant it. You can also keep working on the clone, transferring the best looking myc from each plate untill you no longer see sectoring. You would still want to test your clone once its stabilized
To get the results you are looking for it takes a lot of culture work and most importantly a whole lotta growing each culture out to test
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 Amateur Mycologists United AMU Q&A
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DampMushroom


Registered: 12/01/14
Posts: 115
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Re: FIRST SECTORS/ISOLATES? *DELETED* [Re: mushpunx]
#22423821 - 10/23/15 05:05 PM (8 years, 3 months ago) |
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Post deleted by DampMushroomReason for deletion: I've got to leave for a couple years. Hope the best for all of y'all.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 12 hours
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Quote:
DampMushroom said: Yea a while ago when I tested the plate pins I actually ate a gram from a healthy clean ms pastyplate (was large glad rectangle). There were plenty of clones I grew out and they were onyou ly the strongest pins from the plates with plenty of spore density and it was bruising black also smelled like a strong cube too. There are some pins which just rotted and never colonized mabye it touched bleach or isopropyl during a transfer so I guess what survived is only fruiting substrains which have mostly rhizo characteristics; fast colonizers were my main choice to isolate though everything really needs more time until i find the needle-in-haystack isolate.
Yea man you shouldn't be having iso or bleach anywhere near your culture, it'll contaminate it! Don't really rely on rhizo either, it doesnt mean much.. a rhizo culture will change based on nutrient content in the agar, if you up them it will go fuzzy. But rhizo usually does look the fastest and strongest on a plate so I usually pick it too
Good luck man I hope your grow turns out great!!
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 Amateur Mycologists United AMU Q&A
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