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Offlinesirion
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Registered: 10/14/15
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Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing)
    #22414938 - 10/21/15 06:23 PM (8 years, 3 months ago)

My PF Tek cakes are 17 days post birthing and don't show any signs of pinning, so I wanted to get some input on whether I'm doing anything terribly wrong or just need more patience!

Dates and steps taken:

8/14/15: Inoculated jars (1 syringe Penis Envy, 1 syringe PF amazonian cubensis). Placed in 80° incubator. Cakes took a few weeks to colonize, no contamination.
10/3/15: Took out of incubator and dunked. Cakes felt solid and hard, smelled like mushrooms. Dunking definitely increased their size significantly.
10/4/15: Rolled in verm, put cakes into shotgun fruiting chamber on top of aluminum foil squares. 3-4" of perlite on the bottom. Had some difficulty keeping humidity up, so I've closed ~75% of the holes with tape at this point. Temp is 66-70°.
10/14/15: Posted here, and followed advice to removed tape from my SGFC's holes. That's also when I moved my cakes out and resaturated the perlite, along with trimming the aluminum foil down so more of the perlite was exposed.
10/17/15: Based on advice from Shroomery, started misting the cakes a LOT more, and put my SGFC up on a stand so it got better air flow beneath it (used to only have an inch or two of space below it, now it's completely open). Humidity meter went up to 99% pretty consistently.
10/21/15: Today! Still no pins after 17 days and figured I'd check in and figure out if anyone can offer some suggestions. Supplying a bunch o pictures:












Edited by sirion (10/21/15 06:29 PM)


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OfflineNDStepp84
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Re: Setup critique? (Delayed PFTek pinning - 2.5 weeks after birthing) [Re: sirion]
    #22414965 - 10/21/15 06:30 PM (8 years, 3 months ago)

They look dry to me, are you misting directly until they glisten? Keep misting and be patient.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineMachiavelliavore
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion]
    #22414980 - 10/21/15 06:33 PM (8 years, 3 months ago)

Those still look pretty dry to me.  The fact that there is literally 0 moisture/condensation on your walls suggests that it is not very humid in there at all.

Take off your lady knickers and mist like a man.  If they've been like this a long time, it may be in your interest to dunk them again to rehydrate, I think I saw some bruisering.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (10/21/15 09:53 PM)


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Offlinesirion
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: Machiavelliavore]
    #22415777 - 10/21/15 09:28 PM (8 years, 3 months ago)

Would it make sense to add more verm on top and keep that saturated?

Alternatively, what's involved in re-dunking cakes that you've already dunked and rolled? Do you need to try and remove the verm?

Alternatively alternatively, would it make sense to do that whole drill-hole-insert-straw-keep-straw-full-o-water Tek?


Edited by sirion (10/21/15 09:28 PM)


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InvisibleLocN9ne
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion]
    #22415815 - 10/21/15 09:35 PM (8 years, 3 months ago)

No.

Just dunk.

Entirely unnecessary but up to you


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OfflineNDStepp84
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion]
    #22415817 - 10/21/15 09:36 PM (8 years, 3 months ago)

Feel of them, if they feel really light you could re dunk, but don't add or remove verm. Some people have had success sitting cakes in a small saucer of very shallow water and changing the water every day for a few days.


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Offlinesirion
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: NDStepp84]
    #22416165 - 10/21/15 11:23 PM (8 years, 3 months ago)

Going to apply science: Currently dunking 3 of the cakes and we'll see if they pin sooner than the others.


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OfflineMachiavelliavore
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion]
    #22416184 - 10/21/15 11:29 PM (8 years, 3 months ago)

I think the real point of dunking in this instance is to rehydrate the surface mycellium which has been getting abused for quite some time.  Misting just doesn't rehydrate dried mycellium well, where dunking tends to cause it to super saturate a bit and fluff out with new growth.  Their total water content probably isn't too hot either.

Just do them all IMO.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineGolden Student
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Registered: 10/21/15
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: Machiavelliavore]
    #22416260 - 10/21/15 11:58 PM (8 years, 3 months ago)

I agree to dunk them all. I like the scientific approach but re-hydration couldn't hurt... especially after 17 days w/o pinning.


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Offlinesirion
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: Golden Student]
    #22416463 - 10/22/15 01:56 AM (8 years, 3 months ago)

Alrighty, all cakes are getting dunked. 24 hour or is 12 enough?

Also, whenever I take them out, should I dump verm on top of them? Do a full on roll? Both? In either of those scenarios, should that verm be sterilized in a PC?

(I'll also note that they didn't feel light when picking them up..)


Edited by sirion (10/22/15 01:58 AM)


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OfflineMachiavelliavore
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion] * 1
    #22416525 - 10/22/15 02:41 AM (8 years, 3 months ago)

I imagine 12 will do the trick, especially since they are probably mostly just dry on the surface.

You can re-roll if a bunch of verm fell off, otherwise I'd just leave them as is.  Verm does not need to be treated since it can't really contaminate on its own, being a mineral.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinesirion
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Registered: 10/14/15
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: Machiavelliavore]
    #22416569 - 10/22/15 03:02 AM (8 years, 3 months ago)

I had some issues with rolling, where the verm coverage was really uneven, with some cakes barely having much verm at all on top. I've seen some threads where folks just piled a bunch of verm on top of their cakes and used that as a moisture reservoir...pros and cons of those two options?


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OfflineMachiavelliavore
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion]
    #22416637 - 10/22/15 03:43 AM (8 years, 3 months ago)

Looks like it stuck to yours better than it stuck to mine.  Do what you can, rolling is far from a perfect casing.

Can't say I find the idea of piling verm ontop too compelling.  Seems like it might lead to an uneven pinset, and I wouldn't expect it to be colonized vigorously enough to make it a real resevoir.  If you want to add water capacity, spawn them to coir or case them in a tray.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinesirion
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Registered: 10/14/15
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: Machiavelliavore]
    #22418007 - 10/22/15 12:12 PM (8 years, 3 months ago)

Alrighty, they're all back in their home. Rolled 'em slightly, misted the crap out of them, and we'll see how it all goes.


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OfflineArush
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion]
    #22418313 - 10/22/15 01:22 PM (8 years, 3 months ago)

Quote:

8/14/15: Inoculated jars (1 syringe Penis Envy, 1 syringe PF amazonian cubensis). Placed in 80° incubator. Cakes took a few weeks to colonize, no contamination.




According to RR the middle of cakes are roughly 4 degrees higher than the outside. Above 83 the growth is highly stunted. So assume your cakes were at 84-85. There's a higher chance of contam at those temps as well.


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Offlinesirion
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: Arush]
    #22418871 - 10/22/15 03:38 PM (8 years, 3 months ago)

So is PFTek for simple minds off when they say that 86 is a good temp to be at (and therefore to incubate at 80?)


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OfflineMachiavelliavore
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion]
    #22418911 - 10/22/15 03:51 PM (8 years, 3 months ago)

I think that's old info from an ancient stamets book or something, probably related to growth rate on sterile agar plates.

General concensus seems to be above ~75 you're promoting any contams much more than helping what you're trying to grow.

Under 65 it can get slow, which in its own right can lead to contams germinating/stalling by leaving more sub uncolonized for longer.  One would expect a similar or greater reduction in contaminant growth rate as well so maybe it's a wash.  Regardless, it's just slow.  That might not be a problem if you're doing megaballer inoculation methods such as LI or better yet slurry.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinesirion
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Registered: 10/14/15
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: Machiavelliavore]
    #22422011 - 10/23/15 09:08 AM (8 years, 3 months ago)

Any way to know how soon I can expect pins in this situation?


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InvisibleMr. Alien
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Re: Setup critique? (Delayed PFTek SGFC pinning - 2.5 weeks after birthing) [Re: sirion]
    #22422025 - 10/23/15 09:15 AM (8 years, 3 months ago)

Your SHFC is not properly builded. That's why the condensation. i think the hole pattern is all fucked up.


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