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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
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Clone culture infected with verticillium
#22414272 - 10/21/15 03:48 PM (8 years, 3 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/22413707
share it on advanced mycology maybe someone here knows something
-------------------- SPREAD THE SPORES
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Clone culture infected with verticillium [Re: Aero]
#22414607 - 10/21/15 05:09 PM (8 years, 3 months ago) |
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Looks more mycogone to me but either way, its really hard to clean a culture up from meshed in white molds. I suggest trying a hot pour. Basically put your transfer in the plate and pour the freshly sterilized agar overtop of it. You want the agar to be around 135-140 when you pour. The white mold can't take the heat but the cube myc can sometimes withstand it.
Antibiotics and cooler temps might help as well. I haven't tried that myself but some people have said they had success. I do know that antibiotics can slow molds down (they can also slow the mushroom myc as well if too concentrated). Cool temps really inhibit the molds progression possibly allowing the cubes to get ahead.
Be sure to only take from the very leading edge. Rather than cutting a wedge, try just scraping up the last mm ofvisible myc on the very edge of the visible myc and transfering that.
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
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Re: Clone culture infected with verticillium [Re: Pastywhyte]
#22414649 - 10/21/15 05:18 PM (8 years, 3 months ago) |
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Quote:
Pastywhyte said: Looks more mycogone to me but either way, its really hard to clean a culture up from meshed in white molds. I suggest trying a hot pour. Basically put your transfer in the plate and pour the freshly sterilized agar overtop of it. You want the agar to be around 135-140 when you pour. The white mold can't take the heat but the cube myc can sometimes withstand it.
Antibiotics and cooler temps might help as well. I haven't tried that myself but some people have said they had success. I do know that antibiotics can slow molds down (they can also slow the mushroom myc as well if too concentrated). Cool temps really inhibit the molds progression possibly allowing the cubes to get ahead.
Be sure to only take from the very leading edge. Rather than cutting a wedge, try just scraping up the last mm ofvisible myc on the very edge of the visible myc and transfering that.
thanks a lot for the tips, the strange thing is that i made a few trasnfers before inoculation and it was there.. took a tiny bit from the far edge of the myc well away from the starting point each time but it might have been in there all the time.. its KSSS btw
do u think obtaining some spores from these fruits is an option for a fresh start or i should just go back to the original print ?
-------------------- SPREAD THE SPORES
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