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OfflineRo0k
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Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
Can you help me isolate a strain from these agar plates?
    #22410120 - 10/20/15 04:36 PM (8 years, 3 months ago)

Hello,

The strain I'm working with is B+ and I did a transfer to these 4 plates from 2 nutrient agar plates inocced with a multispore syringe.  The original plates were done ~9/28 and the first transfer was done on 10/10.  The plates are starting to look a little dry and as they've been growing for 10 days, I think it may be time to transfer again.  I did a batch of PDA plates on Sunday.... hope I did that right too. 

But this is my first time trying to isolate... I'm not sure which parts to isolate or how to do it considering they've grown out so well.  Can you recommend where I should try and isolate from?

All 4 plates;



Plate 6



Plate 7



Plate 8



Plate 9



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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Can you help me isolate a strain from these agar plates? [Re: Ro0k]
    #22410517 - 10/20/15 06:09 PM (8 years, 3 months ago)

it would be easier to help if you were further along, all i can say right now is to make transfers from what seems to be the fastest growth for the moment


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Can you help me isolate a strain from these agar plates? [Re: cronicr]
    #22410533 - 10/20/15 06:13 PM (8 years, 3 months ago)

Why does that mycelium look soo whispy? Reminds me of cobweb. :notsureif:


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Can you help me isolate a strain from these agar plates? [Re: Psilosoulful]
    #22410725 - 10/20/15 07:01 PM (8 years, 3 months ago)

Quote:

Psilosoulful said:
Why does that mycelium look soo whispy? Reminds me of cobweb. :notsureif:



first transfer, it happens


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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