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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Contaminated, again; Six out of Six
#22409771 - 10/20/15 03:18 PM (8 years, 3 months ago) |
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...So I made up 6 pf cakes using rye flour, and all of them smell of spoiled milk. There's life in 4/6 of the jars, but I don't have much faith in them. Where did I go so wrong?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22409797 - 10/20/15 03:26 PM (8 years, 3 months ago) |
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How did you sterilize? Rye flour will contain a substancial number of endospores compared to brf which will have very few. Because of this I would want to PC cakes with rye flour for 90 min to be safe.
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22409800 - 10/20/15 03:27 PM (8 years, 3 months ago) |
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Quote:
YaMoonSun said: Where did I go so wrong?
Explain your process. No way to tell you what you did wrong from your post.
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Tehbearz

Registered: 11/04/13
Posts: 88
Last seen: 10 months, 16 days
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Re: Contaminated, again; Six out of Six *DELETED* [Re: YaMoonSun]
#22409803 - 10/20/15 03:27 PM (8 years, 3 months ago) |
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Post deleted by TehbearzReason for deletion: I want it deleted
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Handslikehouses
Lion Skin

Registered: 09/26/15
Posts: 201
Last seen: 2 years, 7 months
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Re: Contaminated, again; Six out of Six [Re: Tehbearz]
#22409807 - 10/20/15 03:28 PM (8 years, 3 months ago) |
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Don't give up man. Don't give contams the satisfaction.
-------------------- Considering spores can survive the vacuum of space, I would assume it's safe to say that mushrooms are on every planet waiting for the right conditions to present themselves. What it all boils down to, is aliens are on shrooms.

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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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What's the timeline on the contamination? I have had some trouble with steamed rye flour based PF substrate. If you're getting decent colonization (say 50%+-25%) then stalling, that sounds more likely to be endospores. If you aren't getting much of anything, it's probably inoculant.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Contaminated, again; Six out of Six [Re: Pastywhyte]
#22409838 - 10/20/15 03:37 PM (8 years, 3 months ago) |
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I followed my standard procedure of steam sterilization in a large pot for 1h30m to 2h30 depending on the size of the jar. I've yet to acquire a pressure cooker. I should probably put that on my Christmas list
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Contaminated, again; Six out of Six [Re: Tehbearz]
#22409846 - 10/20/15 03:38 PM (8 years, 3 months ago) |
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No, I disposed of that LC, it was filthy.
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Jerkypall
Stranger

Registered: 05/21/15
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22418388 - 10/22/15 01:46 PM (8 years, 3 months ago) |
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Just use BRF with a Pressure cooker. Never have I had one contaminate, so long as you let it cook for a while.
Sincerely, Jerkypall
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Contaminated, again; Six out of Six [Re: Jerkypall]
#22418393 - 10/22/15 01:49 PM (8 years, 3 months ago) |
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 ..Yeah man, been meaning to do that for quite some time now
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22418550 - 10/22/15 02:27 PM (8 years, 3 months ago) |
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So should I let the mycelium continue to try to colonize the smells bacterial jars? Or just toss them now and start over? Basically, avoid rye flour until I get a PC, yeah? Or bump up my times to like 3h30m?
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22418565 - 10/22/15 02:29 PM (8 years, 3 months ago) |
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I personally would start over. I don't think increasing the time is going to help? Something smells fishy here and I don't think it's the stove cooking.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Contaminated, again; Six out of Six [Re: JacobStorm]
#22418587 - 10/22/15 02:34 PM (8 years, 3 months ago) |
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Smells more like spoiled milk to me. Could really use some LI in my life
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22418675 - 10/22/15 02:54 PM (8 years, 3 months ago) |
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If you can't get a PC, can't get BRF (check healthfood stores) it might just be best to drop 10-15$ on a couple bags of sterilized rye. Assuming your inoculant is clean (if it's from a reliable vendor it probably is) you could probably save a lot of time and a lot of vendor quality inoculant.
Add a $5 tote and a brick of coir $2.50, and you can prolly pull at least 4 zips for a total of like 20-30$. Sounds fair.
Better than paying .50c an oz or whatever for stupid Bob's Red Mill Rice Flour. BRF is about 2.50$/lbs at a local healthfood store for me, but I live in a Global Center of Diversificated Multiplural Metrohipster.
 inb4 sponsor.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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JacobStorm
psychedelic cartel



Registered: 07/21/15
Posts: 1,499
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I got six pounds of general mill BRF for 18 bucks. But then again I work at a grocery store and got that at cost price.
-------------------- Rogger Rabbits Mushroom growing videos Ethnobotanical garden forum Inocuole tea TEK azur said: If you like 6th grade results, then 6th grade projects are great.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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I generally just buy the whole brown rice and grind it finely in a coffee grinder, but I decided to swap for rye flour, and I've had a fuck-load of contaminates ever since. I was going to blame the vermiculite at first, but yeah. Until I go to the store, I'm going to fuck around with a "new" substrate that I'm curious to see colonized.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22449816 - 10/29/15 04:16 PM (8 years, 3 months ago) |
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Happened again using BRF, so I made a new syringe, and remade the jars for a third imte
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Zuul
Gatekeeper


Registered: 10/11/15
Posts: 49
Loc: Hawaii, BI
Last seen: 8 years, 1 month
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22449914 - 10/29/15 04:37 PM (8 years, 3 months ago) |
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Are you reusing the same jars over and over? With all the different variables it's not going to be easy trying to pin point the cause of contamination. If these cakes don't make it toss everything, get a PC, and get fresh everything. There's a turd in your punch bowl and it's not going to be easy fishing it out.
-------------------- Nothing in this world can take the place of persistence. Talent will not: nothing is more common than unsuccessful men with talent. Genius will not; unrewarded genius is almost a proverb. Education will not: the world is full of educated derelicts. Persistence and determination alone are omnipotent. Calvin Coolidge
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
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Re: Contaminated, again; Six out of Six [Re: YaMoonSun]
#22449915 - 10/29/15 04:37 PM (8 years, 3 months ago) |
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I have never had a cake contaminate before. And Ive never inoculated one inside an SAB only open air.
Id say most of the time cakes contaminate either from wiping the needle down with ISO *after* flame sterilization and the rest of the time its from a bad syringe.
Seeing as you are making your own syringes I would almost gaurentee there is a flaw in your printing or syringe making.
Can you please walk us thru both? Sterile technique included
Working with agar and grain you quickly realize just how often syringes aren't clean enough. Rarely are they clean enough for grains, but can be clean enough for cakes.
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (10/29/15 04:40 PM)
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Contaminated, again; Six out of Six [Re: mushpunx]
#22450015 - 10/29/15 04:57 PM (8 years, 3 months ago) |
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As I was pouring them into the garbage outside, I smelled the dry layer, and it smelled awful, but the substrate below didn't smell as bad.. makes me wonder if I just tossed good jars.. I mean, am I supposed to wait for the jars to turn mushy and gross before I toss them? Maybe using your nose isn't the best idea? I didn't see any signs of mycelium, but it was only a week after inoculation with ms syringe, so 
Quote:
Are you reusing the same jars over and over?
Yes, after a proper washing and steam sterilization 
My print making is pretty basic stuff, I just collect a mushroom that I harvested and twist the cap off gently; Then I put the mushroom gills downward on a piece of foil and cover it with a clean glass for 24 hours.
The syringe making I looked up on a YouTube video; I just don't have a flow-hood, so previously I was making them in my room, and the a/c wasn't working so there was no air-flow in the room if no-body opened the door. Now I have to use a SAB because the damn vents never turn off and people keep the fucking door and windows opened all the time because they love being cold..
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