Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Unopened petri dishes all contamed
    #22409194 - 10/20/15 12:49 PM (8 years, 3 months ago)

So I use wide mouth half pints as petri's. Just cooked some MEA in them for 45 minutes the other day, went to use them and noticed they all had condensation in the center of the agar.

Several of them even had this weird contam I've seen once before. Its like a clear jellyfish substance with lines forming a pattern within it. Hopefully someone can ID it from the picture. It'd be nice to learn more about the organisms that cause this so that I can better know how to avoid it. pics below

As for what went wrong: I've recently started using unmodified plastic lids. Some say they don't form a perfect seal, that may explain the contams. But what about the condensation itself? I was using some agar that had clumped up due to being improperly sealed, perhaps the moisture content was too high and hence some extra water separated from the agar and pooled in the middle?

the lids were also loose after PC'ing...and I didn't use foil because they have no filters...Oh shit was that it? :lol:



Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Unopened petri dishes all contamed [Re: Psilosopherr]
    #22419246 - 10/22/15 05:29 PM (8 years, 3 months ago)

so I used my tried and true PDA with metal lids and this still happened on 3/4 jars. All of the jars it happened to were able to be tightened slightly more...I wouldn't think it would be enough for liquid to get through but that must be whats happening.

all the ones that get this weird contam have extra water on top of the agar layer. Its almost like its a condensation problem because I never notice extra water in there right after the PC cycle.

Or maybe extra water seeps in during the PC cycle, and mixes in well enough that it doesn't re-separate for a while. :shrug:

Maybe I should use foil on the lids even though there's no ports/filters to protect from water??


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Unopened petri dishes all contamed [Re: Psilosopherr]
    #22419293 - 10/22/15 05:37 PM (8 years, 3 months ago)

i would go back to your metal lids with a filter


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Unopened petri dishes all contamed [Re: cronicr]
    #22419312 - 10/22/15 05:40 PM (8 years, 3 months ago)

damn, you think its that the containers are totally closed?


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 16 hours, 32 minutes
Trusted Cultivator
Re: Unopened petri dishes all contamed [Re: Psilosopherr]
    #22420985 - 10/22/15 11:45 PM (8 years, 3 months ago)

If there's no filter, unfiltered air can get pulled in when the pressure equalizes after sterilization so maybe.


--------------------


Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Unopened petri dishes all contamed [Re: Kizzle]
    #22420995 - 10/22/15 11:48 PM (8 years, 3 months ago)

:thumbup: thanks!

Hopefully plastic lids will still work if I add filters. I have about 50/50 metal/plastic lids right now but I really like plastic mainly because they are one piece.


Extras: Filter Print Post Top
OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Unopened petri dishes all contamed [Re: Psilosopherr]
    #22422552 - 10/23/15 11:30 AM (8 years, 3 months ago)

Any idea what contam I got specifically? or is it just "bacteria" in general?


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Unopened petri dishes all contamed [Re: Psilosopherr]
    #22422981 - 10/23/15 01:26 PM (8 years, 3 months ago)

i havent' used plastic lids for my no pours in a long time but i know with glass and metal lids i get no condensation, sometimes i get a littel excess water on top but that goes away in a couple of days


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Jump to top Pages: 1

PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Petri Dish Contam? The One 1,589 4 02/07/05 11:11 AM
by The One
* Contam free MEA upupup 1,537 3 12/12/02 12:28 PM
by upupup
* is this petri contamined? (pic) lepiota 2,140 7 06/18/05 08:37 AM
by drugsaregood
* Bacteria Contam Questions shroomsbury 2,965 7 02/27/02 04:37 PM
by fresh357
* A word about Contams
( 1 2 3 4 all )
WildCardsRevenge 91,261 75 11/04/14 04:06 PM
by TeeRoy87
* Mycelium overcoming contams? Xray_Salami 1,775 2 06/05/02 02:59 PM
by Xray_Salami
* Bacterial Contam in MEA upupup 2,712 7 11/23/02 11:38 AM
by Seuss
* ever order contamed syringes? Shaw 1,081 7 12/28/03 12:50 AM
by catalyst777

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
613 topic views. 42 members, 255 guests and 39 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.01 seconds on 14 queries.