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OfflineNoahr66
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Registered: 09/29/15
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a few questions about sterilization, getting started, and inoculation
    #22409176 - 10/20/15 12:46 PM (8 years, 3 months ago)

hey guys, I ordered a 10 mm golden teacher spore syringe and I am using a low budget version of the PF tek (Please dont judge me as I have no extra money) and I have some quick questions. so first off, I have two cups of brown rice flour with shredded rags (I couldnt find anywhere nearby that has vermiculite because its already winter where i live and I have seen someone say before that this will work) that have been steam sterilized in a pot with a wet towel on the bottom for 2 hours on medium heat with a single layer of aluminum foil. they have been sitting in the pot for about an hour and a half in the pot now without heat.

so I Know now that I should have probably used multiple layers of aluminum foil before putting them in the pot for sterilization, and was wondering if there was any way to sterilize a few extra layers of aluminum foil safely before adding them to the layer, or if the extra layers need to be sterilized at all?

how long should I wait before inocculating (roughly)? Im on a very tight schedule and I dont want all the stuff to be laying out after 4:30 (in about 2 hours)

if I add more than the standard 1-2 ccs of spores to the substate, will they grow faster/stronger/bigger?

If I do use 1-2 ccs of spores per cup would the leftover spores be safe to keep for another time or would the syringe be contaminated from already using it?

I will admit it, I was rushing a little bit to get all this started and it probably wasnt wise to start this without the proper equipment, but its started now so there is nowhere to go but forward with what I have. thanks for any help, I have grown marijuana before but this is my first mushroom grow and I am extremely nervous something will go wrong, or the mushrooms will never grow.


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Edited by Noahr66 (10/20/15 02:10 PM)


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OfflineYaMoonSun
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Registered: 10/23/14
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Re: a few questions about sterilization, getting started, and inoculation [Re: Noahr66]
    #22409295 - 10/20/15 01:15 PM (8 years, 3 months ago)

It's recommended that you remove your sponsor name from the post, and use the search bar at the top of the page. That being said. I've had succeed replacing the vermiculite with spent coffee grounds. It takes a bit longer to colonize, but produces more flushes than usual.

How long you wait depends on the size of the jar. If you sterilized the jar thoroughly, 6-8 hours is not out of the question. I generally make a batch of jars one day, and do all my inoculations the next morning. I find that working on a good night's sleep helps keep you on your game.

If you add more than 1cc, you're likely to get a bacterial contamination because you'll have ruined the moisture content.


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InvisibleJacobStorm
psychedelic cartel
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Registered: 07/21/15
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Re: a few questions about sterilization, getting started, and inoculation [Re: YaMoonSun]
    #22409337 - 10/20/15 01:22 PM (8 years, 3 months ago)

get that vendor name out of the post and we'll talk.


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OfflineNoahr66
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Re: a few questions about sterilization, getting started, and inoculation [Re: JacobStorm]
    #22409525 - 10/20/15 02:12 PM (8 years, 3 months ago)

Done. sorry, im new here and forgot.


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OfflineTehbearz

Registered: 11/04/13
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Re: a few questions about sterilization, getting started, and inoculation *DELETED* [Re: Noahr66]
    #22409770 - 10/20/15 03:18 PM (8 years, 3 months ago)

Post deleted by Tehbearz<p>Reason for deletion: I want it deleted


Edited by Tehbearz (10/21/15 05:57 PM)


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OfflineNoahr66
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Re: a few questions about sterilization, getting started, and inoculation [Re: Tehbearz]
    #22409819 - 10/20/15 03:32 PM (8 years, 3 months ago)

Quote:

Tehbearz said:
I wouldn't trip about only 1 layer of foil. As long as no extra water got in and you pressed the foil on good, you're fine. Like you admitted, I think it woulda been worth the effort to find a verm substitute or even order online, never heard of using rags. Good luck with that, I'll be interested to see how that comes out.

But anyway as soon as the jars are cool enough to be easily handled you can go ahead an inoculate. Wipe down your jars, foil still on, with alcohol and place them in your clean area, still air box, whatever you're working with. Do your flame sterilizations and incoulations. No, giving more than 1-2ccs will not speed the process up more. You can save the syringe for later and try another inoculation but your chances of contam are much higher, it would've been better to just inoculate more of your jars at one time instead. It's still worth a try though, flame sterilize that needle real good when you finish and store it in a sterile environment for later.

Sounds like you're trying to keep everything real small, but in the future if you want to get a lot more yield for your efforts you should look into monotubs. It takes learning a couple new steps, like how to transfer mycelium from jar to jar, and how to pasteurize your substrate (pretty easy stuff), but you'll probably get 10-30x the mushrooms for your effort.




Thanks, When I have a higher budget I am definitely going to make it a bigger project, so I will keep that in mind. I added an extra layer of foil after inoculation because of the holes left in the first layer of foil, hoping that was OK. i also forgot to mention, I added a small amount of ground coffee to the brf, I knew Id heard it somewhere before. A lot went wrong so This whole thing seems like a disaster so far but Ill see how it goes, at least now I know not to eat pot brownies before going about growing mushrooms haha!


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InvisiblePastywhyteMDiscord
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Re: a few questions about sterilization, getting started, and inoculation [Re: Tehbearz]
    #22409833 - 10/20/15 03:36 PM (8 years, 3 months ago)

Part of me is completely fascinated by train wrecks like this. Also I can't help but feel that if someone can't afford anything but a syringe that perhaps they need to focus on things other than cultivation. Anyway I doubt rags are going to hold much moisture so assuming this works you will want to top fruit out of the jar and bottom water. Good luck :thumbup:

:popcorn:


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OfflineTehbearz

Registered: 11/04/13
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Re: a few questions about sterilization, getting started, and inoculation *DELETED* [Re: Noahr66]
    #22409883 - 10/20/15 03:45 PM (8 years, 3 months ago)

Post deleted by Tehbearz<p>Reason for deletion: I want it deleted


Edited by Tehbearz (10/21/15 05:57 PM)


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OfflineNoahr66
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Re: a few questions about sterilization, getting started, and inoculation [Re: Pastywhyte]
    #22409955 - 10/20/15 03:58 PM (8 years, 3 months ago)

ah i bought the syringe about 6 months ago, but shortly after that i got busted for marijuana cultivation and lost my job, and now I work part time as a waiter. I'm definitely looking for more work though.


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OfflineNoahr66
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Re: a few questions about sterilization, getting started, and inoculation [Re: Tehbearz]
    #22410017 - 10/20/15 04:12 PM (8 years, 3 months ago)

Quote:

Tehbearz said:
Quote:

A lot went wrong so This whole thing seems like a disaster so far but Ill see how it goes, at least now I know not to eat pot brownies before going about growing mushrooms haha!




Lol I totally feel you on the stoner grow! My first time felt like a complete disaster too, I had spent so much time reviewing sterile techniques but realized once I got into my still air box I hardly new shit. I was clumsy, bumping jars around and dropping them. I even accidently stabbed myself with the spore syringe so my glove was full of blood and there was blood all over my jars... I thought there was no way anything but mold was going to come out of all this. But sure enough, not one of them contammed, in fact they grew great!

After you've done inoculations, you can leave the jars as they are, no need to make them airtight again. At least, that's with the standard BRF tek where there's a layer of verm on top of the substrate that acts as a filter. In your case that you don't have that verm filter I think the foil is actually a good idea. You wiped the foil down with alcohol first right? Yeah I believe the deal with the coffee grounds is they add a lot of nitrogen to the soil, which fruiting mushrooms like. However, I think it alters pH a little, it might not be so good to add that to cakes or what you're inoculating into. (I doubt it will ruin your grow though, just might increase contam chance slightly) I'd be interested to know how much adding coffee ground affects your final yield though, I'm debating trying coffee grounds in my tubs.




Well thanks, that gave me a lot more confidence. I just realized I had set glass cups out for myself so I could find them when I was stoned, but I still turned out to Use plastic cups on accident because they were "prettier"! When they came out the bottoms were melted into the towel on the bottom, but still felt intact, so it looks like a complete mess. as for the foil, I already put it on and forgot to wipe it down with alcohol... I hope that will be ok. I sprayed lysol in the room prior to the inoculation so hopefully that cleaned it enough because its beeen on there about 1 1/2 hours now


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