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OfflinePsyche delics
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Why are 2 of my jars not gowing anything?
    #22409005 - 10/20/15 11:54 AM (8 years, 3 months ago)

Ok so i inoculated 5 brf jars around 3 weeks ago 2 have mycellium 1 had contamination and 2 have done nothing at all my rooms around 75 degrees in the day but gets around 68 at night, i also steamed sterilized and didnt use a pc. I have no tape on my jars and even flipped one upside because i heard that helps with air exchange. Any ideas on whats causing this?


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OfflineYaMoonSun
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Re: Why are 2 of my jars not gowing anything? [Re: Psyche delics]
    #22409251 - 10/20/15 01:05 PM (8 years, 3 months ago)

Bacteria; Give them a good smell, and if they smell like spoiled milk then toss them. (I just tossed out two of my jars as well)


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OfflineLogicaL ChaosM
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Re: Why are 2 of my jars not gowing anything? [Re: Psyche delics]
    #22409274 - 10/20/15 01:10 PM (8 years, 3 months ago)

Likely something wrong with the substrate, or very low spore count in your syringe.

Got any pics?


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InvisibleJacobStorm
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Registered: 07/21/15
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Re: Why are 2 of my jars not gowing anything? [Re: LogicaL Chaos]
    #22409282 - 10/20/15 01:12 PM (8 years, 3 months ago)

just wait it out.

I've Had jars wait 3 weeks before the first sign.


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InvisiblePastywhyteMDiscord
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Re: Why are 2 of my jars not gowing anything? [Re: YaMoonSun]
    #22409297 - 10/20/15 01:15 PM (8 years, 3 months ago)

You don't flip the jars until they are 95% done and just need a little help with the last bit on the bottom. Now that you have flipped it you are going to want to leave it flipped or risk the dry verm layer becoming compromised.

BTW this keeps coming up regarding low spore counts in syringes. . . Whats a low spore count? 10million? 50 million? There are many reasons he might not have had germination including unviable spores, but a low spore count is not going to be one of them. If you can even see the spores in the syringe that puts the numbers in the hundreds of millions.


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InvisibleJacobStorm
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Registered: 07/21/15
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Re: Why are 2 of my jars not gowing anything? [Re: Pastywhyte]
    #22409359 - 10/20/15 01:26 PM (8 years, 3 months ago)

Quote:

Pastywhyte said:


BTW this keeps coming up regarding low spore counts in syringes. . . Whats a low spore count? 10million? 50 million? There are many reasons he might not have had germination including unviable spores, but a low spore count is not going to be one of them. If you can even see the spores in the syringe that puts the numbers in the hundreds of millions.




I never thought of it like that. I've wondered the same thing.


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azur said: If you like 6th grade results, then 6th grade projects are great.


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OfflineLogicaL ChaosM
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Re: Why are 2 of my jars not gowing anything? [Re: JacobStorm]
    #22409392 - 10/20/15 01:35 PM (8 years, 3 months ago)

In my experience, Ive had a whole set of slow growing jars.

It could have been the substrate, but i have pf jars pretty well down now.

I believe there were just not much spores in the syringe. Took more than a week for some tiny germination.


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InvisiblePastywhyteMDiscord
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Re: Why are 2 of my jars not gowing anything? [Re: LogicaL Chaos]
    #22409441 - 10/20/15 01:50 PM (8 years, 3 months ago)

Two spores that germinate and meet could potentially grow and colonize a substrate faster than 200 million. There is a reason people search for isolates, limite genetics, etc. For a spore syringe company to pay someone to perform serial dilutions in an attempt to get the numbers of spores down would not be worth the savings realized on prints. Like I said if you can even see the tiniest spec its easily 10,000,000 spores.

Chances are the spores were either unviable (killed by excessive temps, freezing) or simply just old.


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OfflineDrCrumbs
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Re: Why are 2 of my jars not gowing anything? [Re: Pastywhyte]
    #22409590 - 10/20/15 02:30 PM (8 years, 3 months ago)

ya missed, shot water. Shake syringes well (twiddle them).

Even shaking well if there is paper from the print in the syringe they will clump and make it super hard to separate cause they have started mating (on that little bit of nutes).

re-fire and start again.


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