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InvisibleMycoTao
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Registered: 04/08/15
Posts: 78
LI from clone plate
    #22408915 - 10/20/15 11:31 AM (8 years, 3 months ago)

I tried searching but after reading 50 or so posts I have given up.

I have a fully colonized clone plate from a recent grow. I was thinking to squirt sterile water on to the surface, scrape with the needle, then draw it back in. I seem to remember something along those lines as a successful technique.

What I am fuzzy on is this. If I use a full 60cc syringe, and only use whatever the plate will hold before redrawing the water, will the resulting LI be dense enough or should I go with less water?

Once again, Thanks in advance for any direction that can be offered.


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"The notion of illegal plants is obnoxious and ridiculous..."
Terrence McKenna

"A man can live and be healthy without killing animals for food; therefore, if he eats meat, he participates in taking animal life merely for the sake of his appetite. And to act so is immoral."
Leo Tolstoy


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OfflineAdvanced
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Re: LI from clone plate [Re: MycoTao]
    #22408939 - 10/20/15 11:36 AM (8 years, 3 months ago)

Cut off a piece with a sterilized knife/scalpell and drag it in the grain-glas!  syringes i guess are just for A- spores or B- honeymycel grown in a liquid!


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:> Im learning


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Invisiblewildernessjunkie
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Re: LI from clone plate [Re: Advanced]
    #22409690 - 10/20/15 02:58 PM (8 years, 3 months ago)

It will work. But theres a better way. Check this out:

http://www.shroomery.org/forums/showflat.php/Number/20429745

You'll need a blender, but it works very well.


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OfflineMachiavelliavore
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Re: LI from clone plate [Re: MycoTao]
    #22409720 - 10/20/15 03:07 PM (8 years, 3 months ago)

The blender method is quite excellent if you make it strong enough.  I doubt you could get an effective amount of mycellium into a 60cc, maybe a 12cc.

If this is the first clone plate, you probably dont want to get water on the clone tissue incase there's anything nasty on it.  Personally I doubt this would be faster than noc'ing with agar wedges and giving a good hard 30s shake to distribute the mycellium.  Might be a good way to store cultures though.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisiblemagicMerlin
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Re: LI from clone plate [Re: Machiavelliavore]
    #22411585 - 10/20/15 10:27 PM (8 years, 3 months ago)

The technique you described is shown in Munchauzen's last video in volume 1A

Quote:

Machiavelliavore said:
The blender method is quite excellent if you make it strong enough.  I doubt you could get an effective amount of mycellium into a 60cc, maybe a 12cc.




Huh? I use 180cc and 1 plate to make my Li which is plenty strong. Are you saying there would be a problem aspirating it effectively?


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OfflineMachiavelliavore
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Re: LI from clone plate [Re: magicMerlin]
    #22411744 - 10/20/15 11:27 PM (8 years, 3 months ago)

Sucking it off the plate would have the following disadvantages:

Leave mycellium in bigger chunks, and thus would have lower inoculation point density.

Without the blended agar plate, it may not get rolling as fast since it doesn't come with easily accessible nutes.

I think it would be difficult to effectively aspirate the mycellium off an entire plate.

Having made some blended LI with too little agar n mycellium and having had it take 7 days to even start growing, I wouldn't expect amazing efficacy sucking if off a plate.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleMad Season
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Re: LI from clone plate [Re: Machiavelliavore]
    #22412097 - 10/21/15 01:28 AM (8 years, 3 months ago)

It has a fast leap off. Just like regular blended li. Muda says that slow leap off is because of improper blending (too much blending) in the tek it says to do like 4 seconds tops of blending. In private he says to do 2 or 3 one second bursts. Youre literally just turning it into agar chunks. 7 day leap offs ime are from that. Never had a not enough myc problem..

As for the blenderless li, it may have less overall mycelium, but a lot of inoculation points will form. Myc is quite tiny. You can still dilute it into a large amount of water, and still see good results.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
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No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineMachiavelliavore
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Re: LI from clone plate [Re: Mad Season]
    #22412116 - 10/21/15 01:37 AM (8 years, 3 months ago)

I thought I was pretty careful not to overblend it, but who knows.  I feel like I'd be more comfortable just doing wedges.  Good to know though, I'll drop my blend time to the bare bare bare minimum.  My concern with sucking up mycellium was that big fat rhizo's would stay intact through the process.

Sucking mycellium off a plate seems like a great way to store cultures though.  The lowest nutrtient water should keep the best I'd think.  I was thinking I'd use some of those dental syringes with the tapered curved plastic tip, put it through the PC (I know it can take it, I've done it,) suck up some mycellium, then clamp the tip with pliers and melt the tip shut with a lighter.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (10/21/15 01:38 AM)


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InvisibleMad Season
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Re: LI from clone plate [Re: Machiavelliavore]
    #22412131 - 10/21/15 01:50 AM (8 years, 3 months ago)

Word. I just do wedges and LCs these days. So simple, and all good to me. I wouldn't store in non nutrient water because the myc will go into a dormant state, thus having a slow leap off. Storage of liquid is always best with an lc. But li can turn 1 plate into a bunch of spawn.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineMachiavelliavore
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Re: LI from clone plate [Re: Mad Season]
    #22412141 - 10/21/15 01:56 AM (8 years, 3 months ago)

I made an LC with agar that I thought was good.  Then some floating shit turned up like 1.5 months after a giant clump of mycellium had formed.

I'm digging all the blender shit.  I think LI/wedges for testing and PF slury with a few cakes in the fridge when I get something I like.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Extras: Filter Print Post Top
InvisibleMycoTao
Intrepid Psychonaut
Male

Registered: 04/08/15
Posts: 78
Re: LI from clone plate [Re: Machiavelliavore]
    #22412995 - 10/21/15 09:59 AM (8 years, 3 months ago)

I was thinking about the "scrape and aspirate" method to get a bit more longevity out of this plate.

My line of thinking went if I scrape the aerials from this plate and use that as my LI, in a few days the myc will have recovered sufficiently for me to do so again without having to wait for another plate to colonize from the master.

As to nasties, while I am sure they could be there, I highly doubt it. I made 3 plates from the original specimen, 2 of which had minor bacterial contaminants and one which seemed spotless. Since I was transfering anyway, I made 2 from each of the ones with bacteria and 1 + a ghetto master from the one that showed clean. The 4 that were transferred from the dirty plates grew in clean amd have already been dropped as wedges leaving me with this one and the master.

I'm not too concerned with speed as my room is already near capacity with monos. I am just trying to keep a stock of spawn up so I can rotate when the others give out or compensate for any tubs that might get contaminated.

That being said, the discussion that my question spawned was quite interesting... thanks for that too.
:awethumb:


--------------------
"The notion of illegal plants is obnoxious and ridiculous..."
Terrence McKenna

"A man can live and be healthy without killing animals for food; therefore, if he eats meat, he participates in taking animal life merely for the sake of his appetite. And to act so is immoral."
Leo Tolstoy


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