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 LocN9ne
ɢﾷﾷd ԲﾷЯ ﾢﾷȚￃΙﾢɢ ﾤﾷ￦ﾤΙԲﾼ
Registered: 04/17/15
Posts: 7,062
Loc: to the brain 
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 Pennicillium 
#22407235 - 10/20/15 07:14 AM (5 years, 4 months ago) |
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Alright I got a dish with pennicillium that I wanna take a transfer from... how do I go about doing this, should I isolate both dishes together in my SAB? I've never dealt with this contam on agar before...should I just say fuck that dish or what.
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micro
bunbun has a gungun
Registered: 05/09/03
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Loc: Brick City
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Re: Pennicillium [Re: LocN9ne]
#22407247 - 10/20/15 07:26 AM (5 years, 4 months ago) |
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hmm, we always used ampicillin in plates but i guess it could work
you added it after sterilizing?
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LocN9ne
ɢﾷﾷd ԲﾷЯ ﾢﾷȚￃΙﾢɢ ﾤﾷ￦ﾤΙԲﾼ
Registered: 04/17/15
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Re: Pennicillium [Re: micro]
#22407250 - 10/20/15 07:28 AM (5 years, 4 months ago) |
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Lol my bad on the confusion... it's got pennicillium mold on the agar, let me snap a pic real quick hold up.
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LocN9ne
ɢﾷﾷd ԲﾷЯ ﾢﾷȚￃΙﾢɢ ﾤﾷ￦ﾤΙԲﾼ
Registered: 04/17/15
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Re: Pennicillium [Re: LocN9ne]
#22407258 - 10/20/15 07:34 AM (5 years, 4 months ago) |
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Lol, I'm half assed drunk right now, I almost fell down holding that fucker up to the light for the pic.
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LocN9ne
ɢﾷﾷd ԲﾷЯ ﾢﾷȚￃΙﾢɢ ﾤﾷ￦ﾤΙԲﾼ
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Re: Pennicillium [Re: LocN9ne]
#22407262 - 10/20/15 07:37 AM (5 years, 4 months ago) |
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Check this out... I didn't strain my rye soak water I was using to make that agar, so in turn there is some floaties in it (it settled for over a week in my fridge)
and I an only PC'ing for 30 minutes when I make my agar, could that be causing a problem?
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micro
bunbun has a gungun
Registered: 05/09/03
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Re: Pennicillium [Re: LocN9ne]
#22407274 - 10/20/15 07:42 AM (5 years, 4 months ago) |
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only if you're talking total time
15psi, then count
looks easy but i would use my bunsen burner :V
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 tetherface
get in where you fit in
Registered: 10/05/14
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Re: Pennicillium [Re: LocN9ne]
#22407304 - 10/20/15 08:00 AM (5 years, 4 months ago) |
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I dont think so 30 minutes should be plenty are you doing pour or no pour agar? And I put both petris in a sab at the same time for the transfer just try not to disturb the air much on the tam plate l always open the lid slowly and kinda slide it over enough to sneak my tool in the tsm plate to cut a wedge scoop it up and seal it up as smoothly as youbopened it my thought process about this is that the less air gets stirred up the better
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LocN9ne
ɢﾷﾷd ԲﾷЯ ﾢﾷȚￃΙﾢɢ ﾤﾷ￦ﾤΙԲﾼ
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That's what I did... just a 1 on 1 transfer in the SAB after my other transfers... it went alright I guess, we shall see... I've never took a transfer from an agar plate with a green mold I usually just toss...I pour my dishes tetherface.
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bodhisatta
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Re: Pennicillium [Re: LocN9ne]
#22407379 - 10/20/15 08:29 AM (5 years, 4 months ago) |
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It's a mold contamination so even if you PC for 1 minute it wouldn't be the cause. Anything over 140f for a while would torch the mold.
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micro
bunbun has a gungun
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Wait.
Why did you transfer anything from a petri with green anywhere aside from a different petri?
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bodhisatta
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Re: Pennicillium [Re: micro]
#22407406 - 10/20/15 08:38 AM (5 years, 4 months ago) |
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The mold got in during the inoculation of the dish in question they're satellite colonies not near the transfer. It's from a mess up
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micro
bunbun has a gungun
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word, i always like to be safe
if it looks questionable it'll look much more questionable on a petir dish and a day
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LocN9ne
ɢﾷﾷd ԲﾷЯ ﾢﾷȚￃΙﾢɢ ﾤﾷ￦ﾤΙԲﾼ
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Quote:
bodhisatta said:
The mold got in during the inoculation of the dish in question they're satellite colonies not near the transfer. It's from a mess up
It sure did...
And micro, you don't even wanna know what happened to all my cultures that lead me to transfering from an ugly ass dish like that... I got a stupid AF mock tek started in my journal that I should have followed if that helps...fml
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