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OfflineLogicaL ChaosM
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Can you inject Liquid Culture into PF Jars?
    #22401522 - 10/19/15 08:08 AM (8 years, 3 months ago)

So i want to get into agar work in the future.

I wanna make a first step into the agar world, but i still wanna use the tried and true PF tek cause i know it well.

i was thinking of doing agar, shooting spores on the agar plate, isolate and cut off a nice looking mycelium wedge, adding it to a honey liquid culture.

Then let it colonize the liquid culture, then suck up some liquid culture and inject it into some PF cakes instead of using spores.

Would this work?


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Re: Can you inject Liquid Culture into PF Jars? [Re: LogicaL Chaos] * 1
    #22401535 - 10/19/15 08:16 AM (8 years, 3 months ago)

Of course it will work. But honey is a shitty media choice for LC.


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OfflineLogicaL ChaosM
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Re: Can you inject Liquid Culture into PF Jars? [Re: Pastywhyte]
    #22401542 - 10/19/15 08:19 AM (8 years, 3 months ago)

awesome!

Just wanted to check if it was.

And, i thought it was a common substance for lc's.

Can you recommend a better LC medium?


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Re: Can you inject Liquid Culture into PF Jars? [Re: LogicaL Chaos]
    #22401551 - 10/19/15 08:22 AM (8 years, 3 months ago)

I dislike simple sugar LCs in general but karo and SITR at least make for a clear media and malt extract is even better. Grain soak water will have some sediment and needs a more thorough sterilization cycle but does not caramelize and results in a culture that is not sugar shocked and recovers faster.


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OfflineLogicaL ChaosM
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Re: Can you inject Liquid Culture into PF Jars? [Re: Pastywhyte]
    #22401591 - 10/19/15 08:37 AM (8 years, 3 months ago)

Ok, after some researching, i finally desphered what SITR means.

SITR is Sugar In The Raw.

So making a SITR-based LC is better than honey cause no caramelization, cloudiness or sediements.

instead, a SITR-based LC is clear and thus better.

Is this correct?


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Re: Can you inject Liquid Culture into PF Jars? [Re: LogicaL Chaos]
    #22401606 - 10/19/15 08:42 AM (8 years, 3 months ago)

SITR can still caramelize but has far less sediment. I still don't like SITR LC but its better than honey.


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OfflineLogicaL ChaosM
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Re: Can you inject Liquid Culture into PF Jars? [Re: Pastywhyte]
    #22401626 - 10/19/15 08:50 AM (8 years, 3 months ago)

Alright fair enough.

I had some good sucess with honey lc so i am excited to try out SITR, even thou its not the best.

as long as its decent, im cool with it :shrug:


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Re: Can you inject Liquid Culture into PF Jars? [Re: LogicaL Chaos] * 1
    #22403281 - 10/19/15 12:13 PM (8 years, 3 months ago)

Light major malt powder is the best. Malt: water ratios very greatly. Almost any mixture will work. I use a half teaspoon per quart of water. Some use more, some use less.

It's worth buying if you are planning on growing mushrooms. You will want it for agar


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Re: Can you inject Liquid Culture into PF Jars? [Re: tahoe]
    #22403340 - 10/19/15 12:27 PM (8 years, 3 months ago)

You can make a liquid culture by squirting some water onto the petris, swirl around to pick up mycelium and then suck the water back into the syringe.

The agitated agar dissolves in the water and becomes the substrate.

It's how I make my syringes.


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OfflineLogicaL ChaosM
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Re: Can you inject Liquid Culture into PF Jars? [Re: MacMerdin]
    #22403378 - 10/19/15 12:34 PM (8 years, 3 months ago)

Huh.

So you guys (tahoe included) are saying you can make agar with malt AND make a LC from Agar by adding clean water?

Very interesting...


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Re: Can you inject Liquid Culture into PF Jars? [Re: LogicaL Chaos]
    #22403659 - 10/19/15 01:45 PM (8 years, 3 months ago)

Pasty to make lc with grain soaked water do you simply boil grains and use the leftover water?


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Re: Can you inject Liquid Culture into PF Jars? [Re: Moldy thumb]
    #22403796 - 10/19/15 02:20 PM (8 years, 3 months ago)

Having tried LC very early on, I found them to be quite touchy.  I'm really loving LI and you can noc cakes up with it as well :smile:


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Re: Can you inject Liquid Culture into PF Jars? [Re: Moldy thumb]
    #22403810 - 10/19/15 02:23 PM (8 years, 3 months ago)

Quote:

Moldy thumb said:
Pasty to make lc with grain soaked water do you simply boil grains and use the leftover water?




Essentially yes. I just save the leftover water from grain prep and filter the gypsum and any debris out.


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OfflineLogicaL ChaosM
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Re: Can you inject Liquid Culture into PF Jars? [Re: Machiavelliavore]
    #22404164 - 10/19/15 03:54 PM (8 years, 3 months ago)

Quote:

Machiavelliavore said:
Having tried LC very early on, I found them to be quite touchy.  I'm really loving LI and you can noc cakes up with it as well :smile:




What is "LI"?

And at Tahoe: is there really gympsum inside simmered grain water?


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InvisiblebodhisattaMDiscordReddit
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Re: Can you inject Liquid Culture into PF Jars? [Re: LogicaL Chaos]
    #22404173 - 10/19/15 03:56 PM (8 years, 3 months ago)

LI is a LC without nutrition, it's mycelium added to water, rather than a small amount of mycelium added to nutrient water to grow into more mycelium.


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OfflineLogicaL ChaosM
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Re: Can you inject Liquid Culture into PF Jars? [Re: bodhisatta]
    #22404194 - 10/19/15 04:03 PM (8 years, 3 months ago)

Oh i see. So basically mycelium water.

Im guessing the "I" stands for "Inoculate"

I made this once for ps cyans. Didnt work on the transfer to wood chips, but LI (mycelium water) is really easy to do.

Cant wait to play with agar. :awesome:


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Re: Can you inject Liquid Culture into PF Jars? [Re: LogicaL Chaos]
    #22404212 - 10/19/15 04:10 PM (8 years, 3 months ago)

:thumbup:

you should play with agar first before playing with LC. LC should be made from agar inoculation. LI should be made from colonized agar too.

do other ways work, of course but it's kind of like using a hose clamp somewhere you need a weld.


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Re: Can you inject Liquid Culture into PF Jars? [Re: LogicaL Chaos]
    #22411612 - 10/20/15 10:33 PM (8 years, 3 months ago)

Idk if this will help but this might help you bypass the LC and just go from agar to PF cakes with an LI. http://www.shroomery.org/forums/showflat.php/Number/20429745


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