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InvisibleSupalemonhaze
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Murdering trich.
    #22401279 - 10/19/15 05:42 AM (8 years, 3 months ago)

So I had 2 plates that started showing the bitch trich and I almost threw them away. I kinda stopped to think and remembered you have to boil the plate before opening and then it dawned on me. Why not take the satisfaction of knowing I killed that agar raiding douche with my own 2 hands.

So plate one I burned that mofo alive. Killed most of the mycelium with it also since they were close to each other but growth resumed on the other side after a few days. 2nd spot you see on the bottom was on the lid so that one was more fun seeing it go blacker than the other one.



Plate number 2 developed trich a few days later and I had just bought peroxide but have never used it before. Now since plate one seems like it saw a nuclear holocaust I thought maybe I can do something with peroxide. I knew chances were slim cuz I read that peroxide only kills spores not the actual mold but I guess the trich was still small enough to try. You can see some cracks in the agar between the tape and mycelium. That's where I dropped a drop of 9% peroxide with a syringe right on top the trich. It bubbled for hours and dang it felt good.



I dont really expect to save the samples as one was just a xfer and the other I already took a transfer from it so I dont mind if trich comes back for blood. If it does I wont stop till my plates become black lmao.


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Invisiblemicro
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Registered: 05/09/03
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Re: Murdering trich. [Re: Supalemonhaze]
    #22401381 - 10/19/15 06:42 AM (8 years, 3 months ago)

i almost took a job in alaska studying psychrophilic trichoderma

jesus i'm damn glad i didn't do that


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InvisiblebodhisattaMDiscordReddit
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Re: Murdering trich. [Re: micro]
    #22401383 - 10/19/15 06:43 AM (8 years, 3 months ago)

Just wrap it in tape and throw it in the trash :shrug:


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Loc: 12" down Europe's butthole
Re: Murdering trich. [Re: micro]
    #22401445 - 10/19/15 07:24 AM (8 years, 3 months ago)

Quote:

bodhisatta said:
Just wrap it in tape and throw it in the trash :shrug:




If I threw away every mold contaminated plate id spend more money on plates and jars than my whole setup :p. Boiling is sufficient enough for killing mold spores

Edit: besides both mycelium cultures are still alivr and trich doesnt seem to be growing again. I call that an unexpected win. So far.


Edited by Supalemonhaze (10/19/15 07:25 AM)


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Invisiblemicro
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Re: Murdering trich. [Re: Supalemonhaze]
    #22401460 - 10/19/15 07:30 AM (8 years, 3 months ago)

you know they also make petri dishes out of pyrex, right?


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InvisibleSupalemonhaze
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Registered: 10/02/15
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Loc: 12" down Europe's butthole
Re: Murdering trich. [Re: micro]
    #22401470 - 10/19/15 07:36 AM (8 years, 3 months ago)

I think mine are pyrex. If not pyrex sone other type of glass.

They are called steriplan.


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Invisiblemicro
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Re: Murdering trich. [Re: Supalemonhaze]
    #22401481 - 10/19/15 07:42 AM (8 years, 3 months ago)

so sterilize them :lol:


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InvisibleSupalemonhaze
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Murdering trich. [Re: micro]
    #22401562 - 10/19/15 08:27 AM (8 years, 3 months ago)

Ya that's what I said I will do. No use throwing away a perfectly good petri.

I just did this instead because it felt more fulfilling lol. And if this remains successful I will have 2 more cultures to isolate from


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InvisiblebodhisattaMDiscordReddit
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Re: Murdering trich. [Re: Supalemonhaze]
    #22401611 - 10/19/15 08:45 AM (8 years, 3 months ago)

Didn't notice they were glass. I have 48 pyrex dishes. I use them as coasters and shit now they blow dick compared to styrene disposables


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InvisibleSupalemonhaze
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Loc: 12" down Europe's butthole
Re: Murdering trich. [Re: bodhisatta]
    #22401928 - 10/19/15 09:24 AM (8 years, 3 months ago)

Yes I just bought like 30 disposables on ebay cuz I just realised I need a shitload of agar to isolate 1 strain. And really if you just isolate 1 strain its just wasting time and theres no guarantee its a good isolate.

It would be a bitch to have to buy that many glass petris. I will expand my plate collection one day but for now I spent enough money. Shit I spent over 600 euro in 2 months and haven't even harvested 1 shroom yet except for the grow kit I first bought.( cant really count that as a grow actually.) I wanna see how cultivating works out for me before I dig the hole any deeper


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Invisiblemicro
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Re: Murdering trich. [Re: Supalemonhaze]
    #22407058 - 10/20/15 01:45 AM (8 years, 3 months ago)

I don't think the Pyrex ones close as well as the plastic.


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Murdering trich. [Re: micro]
    #22407089 - 10/20/15 02:15 AM (8 years, 3 months ago)

Thats true I think cos they have quitr a big gap. I suspect this is for expansion while pressure cooking. I notice with mine while they are still warm they are quite difficult to open. Plastic are sterilised by gamma rays if im not mistaken


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Invisiblemicro
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Re: Murdering trich. [Re: Supalemonhaze]
    #22407127 - 10/20/15 03:17 AM (8 years, 3 months ago)

wat...


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Offlinemidnightmaraude
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Re: Murdering trich. [Re: micro]
    #22407432 - 10/20/15 06:48 AM (8 years, 3 months ago)

I don't know if they do sterilize them by gamma. I know some scalpels I bought were sterilized by gamma ray.  Then after I used em I put em in my pc at 23psi wrapped in foil and the plastic caps over the blade melted all over the blade.  :foreheadslap:


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InvisiblebodhisattaMDiscordReddit
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Re: Murdering trich. [Re: midnightmaraude]
    #22407495 - 10/20/15 07:09 AM (8 years, 3 months ago)

Plastic dishes are EO or gamma sterilized.


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