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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Murdering trich.
#22401279 - 10/19/15 05:42 AM (8 years, 3 months ago) |
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So I had 2 plates that started showing the bitch trich and I almost threw them away. I kinda stopped to think and remembered you have to boil the plate before opening and then it dawned on me. Why not take the satisfaction of knowing I killed that agar raiding douche with my own 2 hands.
So plate one I burned that mofo alive. Killed most of the mycelium with it also since they were close to each other but growth resumed on the other side after a few days. 2nd spot you see on the bottom was on the lid so that one was more fun seeing it go blacker than the other one.

Plate number 2 developed trich a few days later and I had just bought peroxide but have never used it before. Now since plate one seems like it saw a nuclear holocaust I thought maybe I can do something with peroxide. I knew chances were slim cuz I read that peroxide only kills spores not the actual mold but I guess the trich was still small enough to try. You can see some cracks in the agar between the tape and mycelium. That's where I dropped a drop of 9% peroxide with a syringe right on top the trich. It bubbled for hours and dang it felt good.

I dont really expect to save the samples as one was just a xfer and the other I already took a transfer from it so I dont mind if trich comes back for blood. If it does I wont stop till my plates become black lmao.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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i almost took a job in alaska studying psychrophilic trichoderma
jesus i'm damn glad i didn't do that
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Murdering trich. [Re: micro]
#22401383 - 10/19/15 06:43 AM (8 years, 3 months ago) |
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Just wrap it in tape and throw it in the trash
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Murdering trich. [Re: micro]
#22401445 - 10/19/15 07:24 AM (8 years, 3 months ago) |
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Quote:
bodhisatta said: Just wrap it in tape and throw it in the trash 
If I threw away every mold contaminated plate id spend more money on plates and jars than my whole setup :p. Boiling is sufficient enough for killing mold spores
Edit: besides both mycelium cultures are still alivr and trich doesnt seem to be growing again. I call that an unexpected win. So far.
Edited by Supalemonhaze (10/19/15 07:25 AM)
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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you know they also make petri dishes out of pyrex, right?
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Murdering trich. [Re: micro]
#22401470 - 10/19/15 07:36 AM (8 years, 3 months ago) |
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I think mine are pyrex. If not pyrex sone other type of glass.
They are called steriplan.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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so sterilize them
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Murdering trich. [Re: micro]
#22401562 - 10/19/15 08:27 AM (8 years, 3 months ago) |
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Ya that's what I said I will do. No use throwing away a perfectly good petri.
I just did this instead because it felt more fulfilling lol. And if this remains successful I will have 2 more cultures to isolate from
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Didn't notice they were glass. I have 48 pyrex dishes. I use them as coasters and shit now they blow dick compared to styrene disposables
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Yes I just bought like 30 disposables on ebay cuz I just realised I need a shitload of agar to isolate 1 strain. And really if you just isolate 1 strain its just wasting time and theres no guarantee its a good isolate.
It would be a bitch to have to buy that many glass petris. I will expand my plate collection one day but for now I spent enough money. Shit I spent over 600 euro in 2 months and haven't even harvested 1 shroom yet except for the grow kit I first bought.( cant really count that as a grow actually.) I wanna see how cultivating works out for me before I dig the hole any deeper
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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I don't think the Pyrex ones close as well as the plastic.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Murdering trich. [Re: micro]
#22407089 - 10/20/15 02:15 AM (8 years, 3 months ago) |
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Thats true I think cos they have quitr a big gap. I suspect this is for expansion while pressure cooking. I notice with mine while they are still warm they are quite difficult to open. Plastic are sterilised by gamma rays if im not mistaken
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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wat...
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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midnightmaraude
Stranger


Registered: 05/09/14
Posts: 1,260
Last seen: 7 years, 1 month
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Re: Murdering trich. [Re: micro]
#22407432 - 10/20/15 06:48 AM (8 years, 3 months ago) |
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I don't know if they do sterilize them by gamma. I know some scalpels I bought were sterilized by gamma ray. Then after I used em I put em in my pc at 23psi wrapped in foil and the plastic caps over the blade melted all over the blade.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Plastic dishes are EO or gamma sterilized.
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