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Edmunter
Mr



Registered: 05/01/13
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PE methods
#22401198 - 10/19/15 04:45 AM (8 years, 3 months ago) |
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Who has found what method most fruitful when growing PE 2015. I have jars colonising, cultures on plates and a LC that I made with a tiger drop/blend.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Cased bulk FTW.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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None. 2015 was the year of the trich
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ProfessorPinHead
Trapped in the Archives....




Registered: 07/09/10
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 Quote:
Pastywhyte said: Cased bulk FTW.

PE does perform better cased!
Then there is the other method... Just do more and don't bother casing. In that case you'd have to make those jars into more jar or even better more bags first though.. lol..
2 substrates uncased will always out perform 1 substrate cased,...
Edited by ProfessorPinHead (10/19/15 08:43 AM)
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Holy crap I haven't seen pinhead in forever!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Indeed. Good to have ya pop by PP
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Edmunter
Mr



Registered: 05/01/13
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Cool. So casing bulk sub seems to be the way to go. Or try Pinheads double grain case thing.
It seems a good idea to clone these guys as it cuts down on blobs?
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Getting a good blob free culture then casing it would be the way to go.
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 18 days, 23 hours
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So my bulk is colonising very quickly, is it worth late casing as I forgot to put a top layer on?
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Case it today
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
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Re: PE methods [Re: azur]
#22499719 - 11/09/15 10:08 AM (8 years, 2 months ago) |
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first flush, and shot of second flush beginning, first flush on racks (over 2.5 dry ounces) grown from multispore, on wbs, spawned to verm/coir/gypsum; no casing layer.....
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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oontribe

Registered: 01/14/15
Posts: 3,570
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I really dont like top layers.
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Edmunter
Mr



Registered: 05/01/13
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What contamination do you think this is? Is it worth just casing on its own?

The others look fine
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Looks like some serious bacteria. You could case and fruit the jars as is if they aren't turning colors. Or if you have enough spawn where it doesn't matter then just toss it (that's personally what I would do).
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Edmunter
Mr



Registered: 05/01/13
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Ive just plain cased that one to see what happens. Just need to get some spores and im roasting along the swapping highway.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,918
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Sweet good luck with it
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 18 days, 23 hours
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Ok, had loads of probs with trich but got one tub rocking and rolling.
So, I have blobs as expected. What is my next move when removing? Are normal style mushrooms expected second flush?
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mushpunx
Fungus Punk



Registered: 04/20/14
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They tend to look more normal on subsequent flushes
Yea of the trich indeed man
--------------------
 Amateur Mycologists United AMU Q&A
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Edmunter
Mr



Registered: 05/01/13
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And whats the best blob harvesting method?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Pick them when they stop growing.
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ProfessorPinHead
Trapped in the Archives....




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Quote:
wowimflabbergasted said: Holy crap I haven't seen pinhead in forever!
I'm still around from time to time.... 
Quote:
Pastywhyte said: Indeed. Good to have ya pop by PP 
Good to see you too Pasty....
Edited by ProfessorPinHead (12/06/15 11:05 AM)
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Edmunter
Mr



Registered: 05/01/13
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So trich came to the party but not befor I made about 10 clones. Roger rabbit says to transfer immedietly you see signs of growth.
Now, should I make a few transfers per clone or just 1?
If I make 1 clone into a liquid culture(under the right conditions) can I prelong the life of the expanding culture.
How many times can you grow clone, grow clone b4 you lose strength ect?
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azur
God of Fuck



Registered: 04/21/12
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Loc: Daid
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How many times? 1 or 100. I wouldn't go more than one as things can go down hill fast
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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Re: PE methods [Re: azur]
#22638876 - 12/10/15 02:39 PM (8 years, 1 month ago) |
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Genetics weaken as you clone?
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Yes, leads quickly to senescence. I don't know how fast, but all my research came back with everyone saying don't clone a clone.
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spacechildo
proletarians rise up


Registered: 01/24/13
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why would you clone a clone when you already have the clone genetics on your first plate? 
makes as many transfers as you feel is needed to say you have a clean culture. mmmm, clean cultures
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I have done it with a working culture just to see what happened. It's not an instant death sentence or nothing but I wouldn't try and create a master out of it nor would I do it more than twice with the same culture.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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If you transfer from a clone, aren't you running the risk of isolating away from the more prominent fruiting strains within? Or is there always enough strains in a clone that a few transfers from the initial clone are reliable?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Sometimes there may be a risk but if you always transfer the healthy growth you usually end up with what you want. I know some people like Lipa believe that the genetics are all fused by that point. If he is correct then it wouldn't even matter.
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Edmunter
Mr



Registered: 05/01/13
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Ok so use the clones and make transfers from the culture. I transfer from a culture basically means it is the same culture and cn live for a long time be slanted ect.....?
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 18 days, 23 hours
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All the clones have turned black and look inactive except for this one. Not sure if is bacteria or not. My cloning technique is terrible. Any tips?
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Mycologist217
Frank's Disciple



Registered: 02/13/13
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My tissue looked tlie that for a couple of days after I cloned it and now it looks like this:

And this:
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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