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OfflineEdmunter
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PE methods
    #22401198 - 10/19/15 04:45 AM (8 years, 3 months ago)

Who has found what method most fruitful when growing PE 2015.  I have jars colonising, cultures on plates and a LC that I made with a tiger drop/blend.


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InvisiblePastywhyteMDiscord
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Re: PE methods [Re: Edmunter]
    #22401388 - 10/19/15 06:47 AM (8 years, 3 months ago)

Cased bulk FTW.


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Re: PE methods [Re: Edmunter]
    #22401512 - 10/19/15 08:01 AM (8 years, 3 months ago)

None. 2015 was the year of the trich


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OfflineProfessorPinHead
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Re: PE methods [Re: Pastywhyte]
    #22401602 - 10/19/15 08:42 AM (8 years, 3 months ago)

:whathesaid:
Quote:

Pastywhyte said:
Cased bulk FTW.




:whathesaid:

PE does perform better cased!

Then there is the other method... Just do more and don't bother casing. In that case you'd have to make those jars into more jar or even better more bags first though.. lol..

2 substrates uncased will always out perform 1 substrate cased,... :smile:


Edited by ProfessorPinHead (10/19/15 08:43 AM)


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Invisiblewowimflabbergasted
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Re: PE methods [Re: ProfessorPinHead]
    #22401651 - 10/19/15 08:58 AM (8 years, 3 months ago)

Holy crap I haven't seen pinhead in forever!


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InvisiblePastywhyteMDiscord
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Re: PE methods [Re: wowimflabbergasted]
    #22401666 - 10/19/15 09:02 AM (8 years, 3 months ago)

Indeed. Good to have ya pop by PP :rockon:


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OfflineEdmunter
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Re: PE methods [Re: Pastywhyte]
    #22412520 - 10/21/15 07:54 AM (8 years, 3 months ago)

Cool.  So casing bulk sub seems to be the way to go.  Or try Pinheads double grain case thing. 

It seems a good idea to clone these guys as it cuts down on blobs?


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Invisiblewowimflabbergasted
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Re: PE methods [Re: Edmunter]
    #22412597 - 10/21/15 08:16 AM (8 years, 3 months ago)

Getting a good blob free culture then casing it would be the way to go.


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OfflineEdmunter
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Re: PE methods [Re: wowimflabbergasted]
    #22499621 - 11/09/15 09:40 AM (8 years, 2 months ago)

So my bulk is colonising very quickly, is it worth late casing as I forgot to put a top layer on?


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Re: PE methods [Re: Edmunter]
    #22499681 - 11/09/15 09:53 AM (8 years, 2 months ago)

Case it today


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OfflineMycologist217
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Re: PE methods [Re: azur]
    #22499719 - 11/09/15 10:08 AM (8 years, 2 months ago)



first flush, and shot of second flush beginning, first flush on racks (over 2.5 dry ounces) grown from multispore, on wbs, spawned to verm/coir/gypsum; no casing layer.....


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Re: PE methods [Re: Mycologist217]
    #22500532 - 11/09/15 01:39 PM (8 years, 2 months ago)

I really dont like top layers.


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OfflineEdmunter
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Re: PE methods [Re: oontribe]
    #22508019 - 11/11/15 05:20 AM (8 years, 2 months ago)

What contamination do you think this is?  Is it worth just casing on its own?



The others look fine



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Invisiblewowimflabbergasted
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Re: PE methods [Re: Edmunter]
    #22508189 - 11/11/15 07:22 AM (8 years, 2 months ago)

Looks like some serious bacteria. You could case and fruit the jars as is if they aren't turning colors. Or if you have enough spawn where it doesn't matter then just toss it (that's personally what I would do).


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Re: PE methods [Re: wowimflabbergasted]
    #22509054 - 11/11/15 12:01 PM (8 years, 2 months ago)

Ive just plain cased that one to see what happens.  Just need to get some spores and im roasting along the swapping highway.


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Re: PE methods [Re: Edmunter]
    #22509076 - 11/11/15 12:09 PM (8 years, 2 months ago)

Sweet good luck with it


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OfflineEdmunter
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Re: PE methods [Re: wowimflabbergasted]
    #22598229 - 12/01/15 09:01 AM (8 years, 1 month ago)

Ok, had loads of probs with trich but got one tub rocking and rolling. 

So, I have blobs as expected.  What is my next move when removing?  Are normal style mushrooms expected second flush?


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Re: PE methods [Re: Edmunter]
    #22598279 - 12/01/15 09:18 AM (8 years, 1 month ago)

They tend to look more normal on subsequent flushes



Yea of the trich indeed man :facepalm:


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Re: PE methods [Re: mushpunx]
    #22599643 - 12/01/15 03:15 PM (8 years, 1 month ago)

And whats the best blob harvesting method?


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Invisiblestareatclouds
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Re: PE methods [Re: Edmunter]
    #22599804 - 12/01/15 03:55 PM (8 years, 1 month ago)

Pick them when they stop growing.


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OfflineProfessorPinHead
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Re: PE methods [Re: wowimflabbergasted]
    #22620599 - 12/06/15 11:02 AM (8 years, 1 month ago)

Quote:

wowimflabbergasted said:
Holy crap I haven't seen pinhead in forever!




I'm still around from time to time....  :penis:



Quote:

Pastywhyte said:
Indeed. Good to have ya pop by PP :rockon:





Good to see you too Pasty.... :smile:


Edited by ProfessorPinHead (12/06/15 11:05 AM)


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OfflineEdmunter
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Re: PE methods [Re: ProfessorPinHead]
    #22637532 - 12/10/15 08:32 AM (8 years, 1 month ago)

So trich came to the party but not befor I made about 10  clones.  Roger rabbit says to transfer immedietly you see signs of growth. 

Now, should I make a few transfers per clone or just 1?

If I make 1 clone into a liquid culture(under the right conditions) can I prelong the life of the expanding culture. 

How many times can you grow clone, grow clone b4 you lose  strength ect?


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Re: PE methods [Re: Edmunter]
    #22638802 - 12/10/15 02:19 PM (8 years, 1 month ago)

How many times? 1 or 100. I wouldn't go more than one as things can go down hill fast


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FALL IN LOVE WITH LC
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OfflineMycologist217
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Re: PE methods [Re: azur]
    #22638876 - 12/10/15 02:39 PM (8 years, 1 month ago)

Genetics weaken as you clone?


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Invisiblestareatclouds
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Re: PE methods [Re: Mycologist217]
    #22638894 - 12/10/15 02:51 PM (8 years, 1 month ago)

Yes, leads quickly to senescence. I don't know how fast, but all my research came back with everyone saying don't clone a clone.


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Re: PE methods [Re: stareatclouds]
    #22638919 - 12/10/15 02:59 PM (8 years, 1 month ago)

why would you clone a clone when you already have the clone genetics on your first plate? :confused2:

makes as many transfers as you feel is needed to say you have a clean culture. mmmm, clean cultures :homerdrool:


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Re: PE methods [Re: stareatclouds]
    #22638924 - 12/10/15 03:00 PM (8 years, 1 month ago)

I have done it with a working culture just to see what happened. It's not an instant death sentence or nothing but I wouldn't try and create a master out of it nor would I do it more than twice with the same culture.


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Invisiblestareatclouds
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Re: PE methods [Re: Pastywhyte]
    #22638944 - 12/10/15 03:06 PM (8 years, 1 month ago)

If you transfer from a clone, aren't you running the risk of isolating away from the more prominent fruiting strains within? Or is there always enough strains in a clone that a few transfers from the initial clone are reliable?


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Re: PE methods [Re: stareatclouds]
    #22638949 - 12/10/15 03:07 PM (8 years, 1 month ago)

Sometimes there may be a risk but if you always transfer the healthy growth you usually end up with what you want. I know some people like Lipa believe that the genetics are all fused by that point. If he is correct then it wouldn't even matter.


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Re: PE methods [Re: Pastywhyte]
    #22641469 - 12/11/15 04:30 AM (8 years, 1 month ago)

Ok so use the clones and make transfers from the culture.  I transfer from a culture basically means it is the same culture and cn live for a long time be slanted ect.....?


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OfflineEdmunter
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Re: PE methods [Re: Edmunter]
    #22653388 - 12/14/15 10:08 AM (8 years, 1 month ago)

All the clones have turned black and look inactive except for this one.  Not sure if is bacteria or not.  My cloning technique is terrible.  Any tips?



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OfflineMycologist217
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Re: PE methods [Re: Edmunter]
    #22653700 - 12/14/15 11:49 AM (8 years, 1 month ago)

My tissue looked tlie that for a couple of days after I cloned it and now it looks like this:


And this:


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My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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