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OfflineProfessorPinHead
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Re: PE methods [Re: wowimflabbergasted]
    #22620599 - 12/06/15 11:02 AM (8 years, 1 month ago)

Quote:

wowimflabbergasted said:
Holy crap I haven't seen pinhead in forever!




I'm still around from time to time....  :penis:



Quote:

Pastywhyte said:
Indeed. Good to have ya pop by PP :rockon:





Good to see you too Pasty.... :smile:


Edited by ProfessorPinHead (12/06/15 11:05 AM)


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OfflineEdmunter
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Re: PE methods [Re: ProfessorPinHead]
    #22637532 - 12/10/15 08:32 AM (8 years, 1 month ago)

So trich came to the party but not befor I made about 10  clones.  Roger rabbit says to transfer immedietly you see signs of growth. 

Now, should I make a few transfers per clone or just 1?

If I make 1 clone into a liquid culture(under the right conditions) can I prelong the life of the expanding culture. 

How many times can you grow clone, grow clone b4 you lose  strength ect?


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Invisibleazur
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Re: PE methods [Re: Edmunter]
    #22638802 - 12/10/15 02:19 PM (8 years, 1 month ago)

How many times? 1 or 100. I wouldn't go more than one as things can go down hill fast


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OfflineMycologist217
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Re: PE methods [Re: azur]
    #22638876 - 12/10/15 02:39 PM (8 years, 1 month ago)

Genetics weaken as you clone?


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Invisiblestareatclouds
star eat clouds?
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Re: PE methods [Re: Mycologist217]
    #22638894 - 12/10/15 02:51 PM (8 years, 1 month ago)

Yes, leads quickly to senescence. I don't know how fast, but all my research came back with everyone saying don't clone a clone.


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Offlinespacechildo
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Re: PE methods [Re: stareatclouds]
    #22638919 - 12/10/15 02:59 PM (8 years, 1 month ago)

why would you clone a clone when you already have the clone genetics on your first plate? :confused2:

makes as many transfers as you feel is needed to say you have a clean culture. mmmm, clean cultures :homerdrool:


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InvisiblePastywhyteMDiscord
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Re: PE methods [Re: stareatclouds]
    #22638924 - 12/10/15 03:00 PM (8 years, 1 month ago)

I have done it with a working culture just to see what happened. It's not an instant death sentence or nothing but I wouldn't try and create a master out of it nor would I do it more than twice with the same culture.


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Invisiblestareatclouds
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Re: PE methods [Re: Pastywhyte]
    #22638944 - 12/10/15 03:06 PM (8 years, 1 month ago)

If you transfer from a clone, aren't you running the risk of isolating away from the more prominent fruiting strains within? Or is there always enough strains in a clone that a few transfers from the initial clone are reliable?


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InvisiblePastywhyteMDiscord
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Re: PE methods [Re: stareatclouds]
    #22638949 - 12/10/15 03:07 PM (8 years, 1 month ago)

Sometimes there may be a risk but if you always transfer the healthy growth you usually end up with what you want. I know some people like Lipa believe that the genetics are all fused by that point. If he is correct then it wouldn't even matter.


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OfflineEdmunter
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Re: PE methods [Re: Pastywhyte]
    #22641469 - 12/11/15 04:30 AM (8 years, 1 month ago)

Ok so use the clones and make transfers from the culture.  I transfer from a culture basically means it is the same culture and cn live for a long time be slanted ect.....?


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OfflineEdmunter
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Re: PE methods [Re: Edmunter]
    #22653388 - 12/14/15 10:08 AM (8 years, 1 month ago)

All the clones have turned black and look inactive except for this one.  Not sure if is bacteria or not.  My cloning technique is terrible.  Any tips?



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OfflineMycologist217
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Re: PE methods [Re: Edmunter]
    #22653700 - 12/14/15 11:49 AM (8 years, 1 month ago)

My tissue looked tlie that for a couple of days after I cloned it and now it looks like this:


And this:


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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