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ProfessorPinHead
Trapped in the Archives....




Registered: 07/09/10
Posts: 4,568
Loc: F=G*m_1*m_2/r^2
Last seen: 4 years, 6 months
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Quote:
wowimflabbergasted said: Holy crap I haven't seen pinhead in forever!
I'm still around from time to time.... 
Quote:
Pastywhyte said: Indeed. Good to have ya pop by PP 
Good to see you too Pasty....
Edited by ProfessorPinHead (12/06/15 11:05 AM)
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 1 hour
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So trich came to the party but not befor I made about 10 clones. Roger rabbit says to transfer immedietly you see signs of growth.
Now, should I make a few transfers per clone or just 1?
If I make 1 clone into a liquid culture(under the right conditions) can I prelong the life of the expanding culture.
How many times can you grow clone, grow clone b4 you lose strength ect?
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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How many times? 1 or 100. I wouldn't go more than one as things can go down hill fast
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 2 hours
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Re: PE methods [Re: azur]
#22638876 - 12/10/15 02:39 PM (8 years, 1 month ago) |
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Genetics weaken as you clone?
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Yes, leads quickly to senescence. I don't know how fast, but all my research came back with everyone saying don't clone a clone.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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why would you clone a clone when you already have the clone genetics on your first plate? 
makes as many transfers as you feel is needed to say you have a clean culture. mmmm, clean cultures
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I have done it with a working culture just to see what happened. It's not an instant death sentence or nothing but I wouldn't try and create a master out of it nor would I do it more than twice with the same culture.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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If you transfer from a clone, aren't you running the risk of isolating away from the more prominent fruiting strains within? Or is there always enough strains in a clone that a few transfers from the initial clone are reliable?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Sometimes there may be a risk but if you always transfer the healthy growth you usually end up with what you want. I know some people like Lipa believe that the genetics are all fused by that point. If he is correct then it wouldn't even matter.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 1 hour
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Ok so use the clones and make transfers from the culture. I transfer from a culture basically means it is the same culture and cn live for a long time be slanted ect.....?
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 1 hour
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All the clones have turned black and look inactive except for this one. Not sure if is bacteria or not. My cloning technique is terrible. Any tips?
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 2 hours
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My tissue looked tlie that for a couple of days after I cloned it and now it looks like this:

And this:
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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