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OfflineAmerican
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Using WBS in PF Tek to Test Isolates * 2
    #22401079 - 10/19/15 02:41 AM (8 years, 3 months ago)

Imagine someone has a ton of isolates. Seriously, like higher than a 1st grader could count.

The truest way to test them is to use the method they will encounter during production. In this case, WBS> Damion's (awesome) 50/50 Coir Verm with Gypsum. Into monotub.

As one wouldn't want to make 329,235,842,633 monotubs JUST to test isolates, could one to try a lil ol' PF Tek jar of each isolate?

But... using BRF for the PF Tek will not be true to the chosen production method (WBS>Coir/Verm/Gypsum), and the isolates could respond differently.:frown:


Does anyone use the PF Tek formula, to test their isolates? I'm sure some people do, as I did UTFSE, but I would like to hear testimonials. Testify, brother!

Does anyone use WBS Flour/verm as a PF Tek substitution?

Is there a BETTER way to fruit  :sagan: of isolates?


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OfflineMachiavelliavore
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Re: Using WBS in PF Tek to Test Isolates [Re: American]
    #22401170 - 10/19/15 04:19 AM (8 years, 3 months ago)

If you wanted to make a small test container representitive of a coir monotub grow, you could simple put prepped WBS and Coir into a jar in the same ratios you would in a tub, sterilize it, and inoculate with LI.  Look up Mudafuka's LI and Bottle tek.  An invitro bottle would probably be most convient, with say 1 cup substrate.

IMO, it's much more about texture than it is about what grain you're using.  A PF cake with any flour is probably going to be about the same.  You can use whole grains mixed with hydrated verm in a PF tek like setup, which would be slightly more representitive.  Also, inoculating a standard PF cake with an isolate requires LI/LC, and will be slower than grains since you can't shake a cake (unless you're spongiform.)

Both of those methods have the disadvantage that LI/LC/Slury needs to be made to inoculate them with an isolate.  In that respect, v-tek is the fastest easiest way to test, though not very representitive of bulk condition.  You can also spawn single jars of spawn to trays or foil shotgun lasagnia pans so you can noc with a wedge.

I wonder if you have ever even looked at damion5050's 80%/20% coir verm tech.

tl;dr Look at invitro v-tek/v-tek, Muda's LI, and Muda's Bottle tek


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinedzavmt
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Re: Using WBS in PF Tek to Test Isolates [Re: Machiavelliavore]
    #22401197 - 10/19/15 04:45 AM (8 years, 3 months ago)

Psst
Great way to dry your grains fast:
Blow dryer


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Invisiblefilthyknees
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Re: Using WBS in PF Tek to Test Isolates [Re: American]
    #22401206 - 10/19/15 04:51 AM (8 years, 3 months ago)

Under 50 fruit in tubs. Agar > grain master > bag > tub

Over 50 fruit in trays, two trays per tub. Agar > grain master > tray > tub

I guess


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


Edited by filthyknees (10/19/15 04:54 AM)


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InvisiblePastywhyteMDiscord
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Re: Using WBS in PF Tek to Test Isolates [Re: dzavmt]
    #22401242 - 10/19/15 05:17 AM (8 years, 3 months ago)

Quote:

dzavmt said:
Psst
Great way to dry your grains fast:
Blow dryer




What the hell does this have to do with the topic at hand :confused2:



If you skip the dry verm layer and use a grain jar lid for the cakes, you can inoculate them with wedges or colonized grain. Still won't shake well but its another option. Also if you do a bulk run in bottles muda style, colonized grain works really good to inoculate.

Personally I prefer to test the method i will be growing with to test isolates. While cakes and the like will definitely show the fruiting potential,  you would be best served to use the method you intend to use most. If you plan to spawn to bulk and case, then spawn to bulk and case. Some scaling down won't hurt too much but keep in mind that smaller substrates typically provide higher BE. Things like cakes bulk bottles and v tek have an advantage of not breaking up the colony so there will be slightly more energy available for fruiting.


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OfflineLogicaL ChaosM
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Re: Using WBS in PF Tek to Test Isolates [Re: Pastywhyte]
    #22401511 - 10/19/15 08:01 AM (8 years, 3 months ago)

You can put colonized grain ontop of a PF jar to replace the dry verm layer?

Whoa. :omgz:

Thats really cool.


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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Re: Using WBS in PF Tek to Test Isolates [Re: LogicaL Chaos]
    #22401532 - 10/19/15 08:14 AM (8 years, 3 months ago)

Quote:

LogicaL Chaos said:
You can put colonized grain ontop of a PF jar to replace the dry verm layer?

Whoa. :omgz:

Thats really cool.




No. You can't.  Read what I wrote again.


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OfflineLogicaL ChaosM
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Re: Using WBS in PF Tek to Test Isolates [Re: Pastywhyte]
    #22401557 - 10/19/15 08:26 AM (8 years, 3 months ago)

I guess im confused on this part: "If you skip the dry verm layer and use a grain jar lid for the cakes, you can inoculate them with wedges or colonized grain."

It sounds like you are saying replace the dry verm layer with a layer of fresh grain then add a wedge of agar or colonized grain.

Can u clear this up for me?


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Invisiblemicro
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Re: Using WBS in PF Tek to Test Isolates [Re: LogicaL Chaos]
    #22401569 - 10/19/15 08:29 AM (8 years, 3 months ago)

someone sectoring for growth would most likely not be using a PF TEK

as far as cubensis goes I never really saw a difference anyway


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InvisiblePastywhyteMDiscord
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Re: Using WBS in PF Tek to Test Isolates [Re: LogicaL Chaos]
    #22401599 - 10/19/15 08:41 AM (8 years, 3 months ago)

Quote:

LogicaL Chaos said:
I guess im confused on this part: "If you skip the dry verm layer and use a grain jar lid for the cakes, you can inoculate them with wedges or colonized grain."

It sounds like you are saying replace the dry verm layer with a layer of fresh grain then add a wedge of agar or colonized grain.

Can u clear this up for me?



The dry verm layer acts as a filter. Grain jar lids have filters on them. The colonized grains do not act as filters so they do not replace the function of the dry verm. The filter on the grain jar replaces the dry verm. Because there is no dry verm layer you are free to inoculate with grains or agar wedges and colonize from the top down. This will take a bit longer but will still work. You can also still use a spore or lc syringe as well if you wanted. The grain jar lid just gives you some more options.


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OfflineLogicaL ChaosM
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Re: Using WBS in PF Tek to Test Isolates [Re: Pastywhyte]
    #22401647 - 10/19/15 08:56 AM (8 years, 3 months ago)

Oh, i see now.

Grain jars are better cause they are verstile.

i got it now.


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OfflineAmerican
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Re: Using WBS in PF Tek to Test Isolates [Re: LogicaL Chaos] * 1
    #22404147 - 10/19/15 03:48 PM (8 years, 3 months ago)

Wow, these are all (well, almost :tongue:) great responses, and there's a lot of teks to read about.

Thanks everybody.

Pasty, I've read a lot of your threads and you always bring the info.

Obviously, spawning to the technique to be employed is most preferable. But do you really think it's going to show that much of a difference over a WBS PF jar?

There is just SO much work involved in making (at least) 1 quart of WBS and a meatloaf tray for all the :sagan: of isolates, that it makes PF tek look much more appealing.

Do you think that the size, shape, and the microclimate/environment characteristics that each test jar/tray has is more important than matching ingredients? In Essence, would a 1/4 size tray be SO much more revealing of genetic proclivity to make it worth the extra work? I mean taking a wedge and slapping it into a PF tek jar is just SO easy :tongue: and space is an issue.


Edited by American (10/19/15 03:50 PM)


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OfflineLogicaL ChaosM
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Re: Using WBS in PF Tek to Test Isolates [Re: American]
    #22404181 - 10/19/15 03:58 PM (8 years, 3 months ago)

I think your last sentence was sarcastic....

If so, i just learned in my thread that an LC can be made from an agar wedge.

Basically, from whati just learned, u take an agar wedge, put it inside an empty petri dish, add some fresh water, dissolve agar/mycelium into water, then suck up using a syringe. Then inject agar LC into jars.

Sounds pretty simple to me :shrug:


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OfflineAmerican
Fuck yeah!

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Re: Using WBS in PF Tek to Test Isolates [Re: LogicaL Chaos]
    #22404251 - 10/19/15 04:20 PM (8 years, 3 months ago)

Thanks for the tip, seems like an interesting approach. But actually, I wasn't being sarcastic.

Using Tyvek instead of a verm layer, and having a good flow hood or SAB would make adding a wedge to PF jars pretty straight forward. At least easier than wedge>WBS>monotub.


Edited by American (10/19/15 04:22 PM)


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InvisiblePastywhyteMDiscord
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Re: Using WBS in PF Tek to Test Isolates [Re: American]
    #22404303 - 10/19/15 04:37 PM (8 years, 3 months ago)

I say if you took the time to make :sagan: of isolates, you might as well find the one thats going to fruit best for the method you plan to grow it with. Sure the difference between some of them may be slight. But add up enough little things and they start to become big things.

Also consider that there are lots of little nuances between strains. Some will be more tolerant of co2, some will not. Some might react poorly to over nutritious substrates some might do better with high spawn rates. A big part of growing a kick ass clone or strain to its max potential is learning what it prefers. A good performing culture will do pretty good no matter what but, why not find whats going to suit your growing methods the best. Thats what commercial edible growers do to make the most of their grows.


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Re: Using WBS in PF Tek to Test Isolates [Re: Pastywhyte]
    #22404336 - 10/19/15 04:47 PM (8 years, 3 months ago)

:whathesaid:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineAmerican
Fuck yeah!

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Re: Using WBS in PF Tek to Test Isolates [Re: cronicr]
    #22409733 - 10/20/15 03:10 PM (8 years, 3 months ago)

Noice. Thanks, Pasty, et all.

:youthemandawg:


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