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OfflineSivarted
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Odds on getting anything viable out of this syringe?
    #22400891 - 10/19/15 12:34 AM (8 years, 3 months ago)



APE syringe from a sponsored vendor.  Presumably it has spores in it as well as whatever the hell is growing in it, I'm going to try to get something on agar, but I'm obviously not super hopeful.  oO

Probably just going to end up waiting for a replacement.


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InvisibleLocN9ne
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Re: Odds on getting anything viable out of this syringe? [Re: Sivarted]
    #22400902 - 10/19/15 12:44 AM (8 years, 3 months ago)

What's wrong with it?


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OfflineSivarted
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Re: Odds on getting anything viable out of this syringe? [Re: LocN9ne]
    #22400945 - 10/19/15 01:15 AM (8 years, 3 months ago)

Quote:

LocN9ne said:
What's wrong with it?




Other than the huge growth of what looks like some kind of mold in it?  Nothing I guess.  Definitely does not look like a spore clump though.  :smile:

And from what I've read, APE spores are supposed to be clear, and practically invisible in water.


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OfflineMachiavelliavore
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Re: Odds on getting anything viable out of this syringe? [Re: Sivarted]
    #22400989 - 10/19/15 01:41 AM (8 years, 3 months ago)

Yeah that looks pretty bad.  If you can take a single drop and put it on agar, then get the spore company to send a replacement.  Whatever that huge clump is, if it's living it's probably gonna stomp all over some cube spores.

Shit, that even fucks it up for microscopy :smile:

Maybe inject some into a roommate and see if you get the fluke man from the xfiles.


Oh yeah, they make those PE syringes by centrifuging ground caps, maybe some other crap from htat process got sucked in.  It might be inert.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (10/19/15 01:41 AM)


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Invisiblewowimflabbergasted
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Re: Odds on getting anything viable out of this syringe? [Re: Machiavelliavore]
    #22401657 - 10/19/15 09:00 AM (8 years, 3 months ago)

Try it and see dude the APE syringes that I had all had white shit in them not black spores. Not sure why I never grew them.


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OfflineSivarted
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Re: Odds on getting anything viable out of this syringe? [Re: wowimflabbergasted]
    #22403065 - 10/19/15 11:10 AM (8 years, 3 months ago)

APE doesn't have black spores from what I've read, they're transparent.  I dunno.  Whatever that glob is, it's not spores.  I know what spore clumps look like.  Still going to give it a shot.  Knocked up a brf jar and a couple agar plates, we'll see.


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OfflineSivarted
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Re: Odds on getting anything viable out of this syringe? [Re: Sivarted]
    #22418021 - 10/22/15 12:15 PM (8 years, 3 months ago)

Update from the vendor:

Quote:

Thanks for writing.

The sample you received should be OK. The spore material used for APE is not dark purple/black like most of Ps. cubensis... it is much lighter, lacking the pigments. So when the spore clusters clump up like this it can be a bit nasty looking. If you find the material to be contaminated after performing microscopy, please let us know!




Not really convinced, because I've seen spore clusters before, and that does *not* look like a spore cluster (plus it's a really nasty looking brown), but since APE doesn't actually drop spores maybe it's fine and that's just material that goes in the syringe with the spores?  I dunno.  We'll see.

I inoculated one brf jar and two agar plates, the agar this morning just started showing tiny dots of what might be myc, so hopefully even if the syringe is as contaminated as it looks, I can at least do some transfers on it. :smile:


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Invisiblewowimflabbergasted
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Re: Odds on getting anything viable out of this syringe? [Re: Sivarted]
    #22419633 - 10/22/15 06:49 PM (8 years, 3 months ago)

Its normal. APE syringes aren't going to look like other ones you've seen.


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InvisibleMad Season
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Re: Odds on getting anything viable out of this syringe? [Re: wowimflabbergasted]
    #22419700 - 10/22/15 07:01 PM (8 years, 3 months ago)

:whathesaid: looks like a syringe from a certain vendor with a centrifuge.. :wink:. All the syringes I've gotten from them have huge globs of spores in the syringe like that. I'd bet all in that that is a great ape spore syringe right there.

Actually.. Its bad. Send it to me to make sure :wink:


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Invisiblewowimflabbergasted
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Re: Odds on getting anything viable out of this syringe? [Re: Mad Season]
    #22419708 - 10/22/15 07:02 PM (8 years, 3 months ago)

:lol:


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Invisibletahoe
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Re: Odds on getting anything viable out of this syringe? [Re: wowimflabbergasted]
    #22420793 - 10/22/15 10:51 PM (8 years, 3 months ago)

Don't assume that syringe is bad. It does look funky but you can't judge them visibly.
Try 2-3 jars.

Had that syringe been used before?


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OfflineMajickMuffin
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Re: Odds on getting anything viable out of this syringe? [Re: tahoe]
    #22420818 - 10/22/15 10:56 PM (8 years, 3 months ago)

Looks like a liquid culture .

What if somehow the spores have germinated and are forming a mycelium network inside there. Company made mistake ?


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InvisibleMad Season
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Re: Odds on getting anything viable out of this syringe? [Re: MajickMuffin]
    #22420828 - 10/22/15 10:57 PM (8 years, 3 months ago)

It looks like albino spores to me lol. Albinoism effects spores too. This is why aa+ isnt a true albino. But leucistic A+ isn't anywhere near as cool haha


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Edited by Mad Season (10/22/15 10:58 PM)


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InvisiblePastywhyteMDiscord
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Re: Odds on getting anything viable out of this syringe? [Re: Mad Season]
    #22420923 - 10/22/15 11:25 PM (8 years, 3 months ago)

My APE syringe germinated while being stored in the fridge. Looks quite similar. Just tested it and have good growth.


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OfflineSivarted
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Re: Odds on getting anything viable out of this syringe? [Re: Pastywhyte]
    #22421309 - 10/23/15 02:41 AM (8 years, 3 months ago)

I have tests underway.  That big clump got stuck in the needle and the whole thing shot out at once though.  Presumably there are still spores in the syringe though.  Oops.  :smile:

The agar plates I did showed their first signs of white though, so it seems like everything is good.


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InvisibleInocuole
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Re: Odds on getting anything viable out of this syringe? [Re: Sivarted]
    #22421350 - 10/23/15 03:30 AM (8 years, 3 months ago)

All that not being convinced for what? :lol:


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OfflineLogicaL ChaosM
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Re: Odds on getting anything viable out of this syringe? [Re: Sivarted]
    #22421748 - 10/23/15 07:34 AM (8 years, 3 months ago)

That sure is strange. Never seen that before.

Then again, ive never grown an albino strain either :shrug:


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Invisiblewowimflabbergasted
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Re: Odds on getting anything viable out of this syringe? [Re: Inocuole]
    #22421755 - 10/23/15 07:38 AM (8 years, 3 months ago)

Quote:

Inocuole said:
All that not being convinced for what? :lol:



:whathesaid::lmafo:


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Offlineblackout
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Re: Odds on getting anything viable out of this syringe? [Re: MajickMuffin]
    #22427920 - 10/24/15 03:53 PM (8 years, 3 months ago)

Quote:

MajickMuffin said:
What if somehow the spores have germinated and are forming a mycelium network inside there. Company made mistake ?


I have read that the spores can cannibalise their own tissues. With poor spore droppers I expect gills might be in the spore solution.

I have gotten exotics with visible myc growing in them, never cubes, and I have gotten far more cube syringes over the years.


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Re: Odds on getting anything viable out of this syringe? [Re: blackout]
    #22427932 - 10/24/15 03:55 PM (8 years, 3 months ago)

It would suck if that's what grew out instead of the spores, and it happened to be from a clone culture near the end of its life.  With something like APE that's actually somewhat likely to be the case, at least comparatively to other varieties.  Hopefully some spores would germinate and integrate into the genetics and keep that from being an issue.


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