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GoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
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Alcohol Cotton Balls
#22400684 - 10/18/15 10:58 PM (8 years, 3 months ago) |
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I will be inoculating soon does anyone recommend the idea of dipping cotton balls in Isoprop Alc then placing them over inoculation holes and sticking spore syringe through swab then into jar. Seems like alchohol might get into the jar but maybe it will stay on top of dry verm layer. I don't so I'm asking your opinions/experiences thanks, best of luck.
-------------------- Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money. But hell life's right in front of me, nothing to look forward to. Best of luck to all.
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HappyNoob
Stranger

Registered: 09/28/15
Posts: 56
Last seen: 7 years, 11 months
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Alcohol wipe > flame sterilize > inject is what i do Repeat for every injection
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: Alcohol Cotton Balls [Re: HappyNoob]
#22400717 - 10/18/15 11:14 PM (8 years, 3 months ago) |
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Yea alcohol is not sterile nor does it sterilize, only used to sanitize.. If you push a flame sterilized syringe thru an alcohol soaked swab it would no longer be sterile.
Just flame sterilize between holes or at least between jars
--------------------
 Amateur Mycologists United AMU Q&A
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 19 hours
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Quote:
GoldenGrowBoy said: I will be inoculating soon does anyone recommend the idea of dipping cotton balls in Isoprop Alc then placing them over inoculation holes and sticking spore syringe through swab then into jar. Seems like alchohol might get into the jar but maybe it will stay on top of dry verm layer. I don't so I'm asking your opinions/experiences thanks, best of luck.
Sticking a flame sterilized syringe into an unsterile substance like alcohol is like wiping shit all over your face after a shower....
Flame needle till red hot, expunge a drop or 2 to cool needle, immediately inoculate media.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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MrSturgill
I'm a damn genuis! Just ask me




Registered: 08/31/15
Posts: 840
Loc: Somewhere over the rainbo...
Last seen: 4 months, 17 days
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Re: Alcohol Cotton Balls [Re: PussyFart]
#22400820 - 10/18/15 11:54 PM (8 years, 3 months ago) |
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Quote:
GoldenGrowBoy said:
Sticking a flame sterilized syringe into an unsterile substance like alcohol is like wiping shit all over your face after a shower....
Flame needle till red hot, expunge a drop or 2 to cool needle, immediately inoculate media.
Man i did not know that, i always wipe my needle with an alcohol soaked paper towel to cool it down before sticking it in my jar. Thanks for the tip.
-------------------- All You Need
Stuff and Things Read, read, read some more, don't consider anything has been read until everything has been read, then go back and read it again.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
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Re: Alcohol Cotton Balls [Re: PussyFart]
#22400880 - 10/19/15 12:28 AM (8 years, 3 months ago) |
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Quote:
PussyFart said:
Quote:
GoldenGrowBoy said: I will be inoculating soon does anyone recommend the idea of dipping cotton balls in Isoprop Alc then placing them over inoculation holes and sticking spore syringe through swab then into jar. Seems like alchohol might get into the jar but maybe it will stay on top of dry verm layer. I don't so I'm asking your opinions/experiences thanks, best of luck.
Sticking a flame sterilized syringe into an unsterile substance like alcohol is like wiping shit all over your face after a shower....
Flame needle till red hot, expunge a drop or 2 to cool needle, immediately inoculate media.
Couldn't you just flame the needle and go straight to inoculation? It's going to cool as the first drops come out... doing the 2 drops then inoculating doesn't have any apparent advantage to me.
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: Alcohol Cotton Balls [Re: invitro] 1
#22400944 - 10/19/15 01:15 AM (8 years, 3 months ago) |
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I don't use needles much but when I did I always slammed em hot.
-------------------- Trade List 🖕🖕🖕 6 hole Mini Monos
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MrSturgill
I'm a damn genuis! Just ask me




Registered: 08/31/15
Posts: 840
Loc: Somewhere over the rainbo...
Last seen: 4 months, 17 days
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Re: Alcohol Cotton Balls [Re: invitro]
#22400953 - 10/19/15 01:20 AM (8 years, 3 months ago) |
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How embarrassing, a simple search would've informed me of my wrong doings. -smh- live and learn i guess.
-------------------- All You Need
Stuff and Things Read, read, read some more, don't consider anything has been read until everything has been read, then go back and read it again.
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MrSturgill
I'm a damn genuis! Just ask me




Registered: 08/31/15
Posts: 840
Loc: Somewhere over the rainbo...
Last seen: 4 months, 17 days
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Re: Alcohol Cotton Balls [Re: invitro]
#22400962 - 10/19/15 01:26 AM (8 years, 3 months ago) |
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Quote:
invitro said: Couldn't you just flame the needle and go straight to inoculation? It's going to cool as the first drops come out... doing the 2 drops then inoculating doesn't have any apparent advantage to me.
Quote:
RogerRabbit said: If you flame sterilize, anything you do afterward will only make the needle 'dirtier'. Why not just flame and use? Forget the alcohol after flaming. There is no need to cool down the needle. Just use hot and let the first drop or two of solution cool the needle, so the rest can flow contaminant free.
Hope this answers your question
-------------------- All You Need
Stuff and Things Read, read, read some more, don't consider anything has been read until everything has been read, then go back and read it again.
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GoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
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Re: Alcohol Cotton Balls [Re: MrSturgill]
#22403491 - 10/19/15 01:02 PM (8 years, 3 months ago) |
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Thanks a lot super informative as usual.
-------------------- Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money. But hell life's right in front of me, nothing to look forward to. Best of luck to all.
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