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dynamitehack
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Registered: 09/11/15
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Last seen: 8 years, 25 days
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HELP! inoculation and PF Tek
#22394847 - 10/17/15 07:09 PM (8 years, 3 months ago) |
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Hey guys, So I'm on my second grow and I have different syringes from the first time. And they don't fit in the holes in lid that I made!!!
What would you guys do?
I have two options I think.... A.) Make the holes larger with a larger nail. I think I could do it without unscrewing any of the jars, just hammer a nail that's a little larger. In this case, could I just pressure cook the jars again and they'd be re-sterilized?
B.) Get all of the spores out of the big syrings, and suck it back up into my little syringes and proceed that way. Do you think that would be a cause for contaminants? Because the spores would be in a bowl for a little bit? Or would flaming the syringe take care of that?
THANK YOU!
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394885 - 10/17/15 07:17 PM (8 years, 3 months ago) |
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Gently enlarge the holes... don't go so deep as to disturb the Verm layer... or, make new jars...brf and Verm are cheap af.
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Q&A US vs. THEM The more I learn, the less I know.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394892 - 10/17/15 07:18 PM (8 years, 3 months ago) |
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You're not flaming the bowl so don't shoot spores into it. If you make bigger holes you mess up the dry verm layer and that leaves the possibility that a second sterilization will mess up the moisture content of the cakes. I'm not sure what I'd do but making a completely new batch of cakes is the only method that's not threatened by some kind of potential failure.
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dynamitehack
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Re: HELP! inoculation and PF Tek [Re: Inocuole]
#22394909 - 10/17/15 07:22 PM (8 years, 3 months ago) |
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When you say "mess up the dry verm layer", do you mean messing it up by touching it with a dirty nail? Or messing it up by making indents? I think i could be careful enough to not make indents into the verm toooooo much, and I could also flame the nail.
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Inocuole
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394913 - 10/17/15 07:23 PM (8 years, 3 months ago) |
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Messing it up by shaking it loose or disturbing it in any way really. Touching it, accidentally slipping grip on the jar and giving it a tiny shake, any of that kinda thing and I'd be paranoid as hell.
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dynamitehack
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Re: HELP! inoculation and PF Tek [Re: Inocuole]
#22394920 - 10/17/15 07:26 PM (8 years, 3 months ago) |
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Also, you said "you're not flaming the bowl", but when making syringe prints, you scrape the spores into a bowl, add distilled water, and suck it up. Of course the bowl is wiped with alcohol, but isn't it pretty sterile at that point?
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394932 - 10/17/15 07:29 PM (8 years, 3 months ago) |
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Alcohol does not sterilize it only sanitizes...and that is most definitely not the whole process when making a GOOD syringe.
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Q&A US vs. THEM The more I learn, the less I know.
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Inocuole
Scalpel of Evil's Bane



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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394934 - 10/17/15 07:29 PM (8 years, 3 months ago) |
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Alcohol is a sanitizer, so any mold spores would survive it. It would be a good amount better if you actually sterilized a shotglass or something like that, with foil over it, and used that in a still air box so it actually was sterile when you pulled off the foil. If the bowl in question is super tiny, and it can survive a PC cycle, that'd be perfect.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: HELP! inoculation and PF Tek [Re: Inocuole]
#22394935 - 10/17/15 07:30 PM (8 years, 3 months ago) |
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Quote:
Inocuole said: Alcohol is a sanitizer, so any mold spores would survive it. It would be a good amount better if you actually sterilized a shotglass or something like that, with foil over it, and used that in a still air box so it actually was sterile when you pulled off the foil.
This.
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Q&A US vs. THEM The more I learn, the less I know.
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dynamitehack
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Re: HELP! inoculation and PF Tek [Re: Inocuole]
#22394941 - 10/17/15 07:33 PM (8 years, 3 months ago) |
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Shit. Alright guys, I'm going to try with a nail VERY carefully and if I can not disturb the verm then I'm going to leave it. If I feel iffy, then I'll just start over with new substrate.
I have 5 syringes back up so I may just do this as an experiment.
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Machiavelliavore
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Re: HELP! inoculation and PF Tek [Re: Inocuole]
#22394957 - 10/17/15 07:35 PM (8 years, 3 months ago) |
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I'd use a nice tapered punch if you have one. Dip the tip in alcohol and give it a healthy flaming, then gently englarge the holes, which you can probably do without a hammer causing all that vibration. Do it in an air box.
You could also just take the lids off in a still air box and inoculate with the syringe at an angle to try to avoid having your hands above the jars. I mean the verm layer should keep it clean enough even if your technique isn't perfect.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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dynamitehack
Stranger
Registered: 09/11/15
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Last seen: 8 years, 25 days
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394958 - 10/17/15 07:35 PM (8 years, 3 months ago) |
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Wait....how could I go about sterilizing a shot glass? Boiling it? Flaming it?
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394964 - 10/17/15 07:37 PM (8 years, 3 months ago) |
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Why start the process with an error... dump the shit out, fix the lids...spend the 5 minutes it takes to whip up another batch, pc them motherfuckers..and begin without questioning it... you haven't even inoculated yet and you are gonna add a variable for failure... makes no sense.
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Q&A US vs. THEM The more I learn, the less I know.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394967 - 10/17/15 07:37 PM (8 years, 3 months ago) |
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If you don't have a pressure cooker forget I said it. The idea about inoculating in a still air box by taking off the lids might be even better than either of the previous ones.
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dynamitehack
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Registered: 09/11/15
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Last seen: 8 years, 25 days
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Re: HELP! inoculation and PF Tek [Re: LocN9ne]
#22394980 - 10/17/15 07:39 PM (8 years, 3 months ago) |
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I mean, it is more than 5 minutes. 30 minutes to get the ingredients, 20 minutes to mix it up and fill the jars, and 90 minutes to pressure cook.
I think I will go with that though. Thanks for all of your help.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22394986 - 10/17/15 07:39 PM (8 years, 3 months ago) |
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If you're going the nail route, be sure and dip it in alcohol and flame it as well.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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dynamitehack
Stranger
Registered: 09/11/15
Posts: 20
Last seen: 8 years, 25 days
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22395000 - 10/17/15 07:42 PM (8 years, 3 months ago) |
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WAIT GUYS.
You can remove the needle tip from both syringes.
Can I just switch them out??!?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22395008 - 10/17/15 07:44 PM (8 years, 3 months ago) |
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If they're both Luer-lock, yeah, you could do that. I mean, do it in a still air box if you can, then flame sterilize the needle before using it.
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dynamitehack
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Re: HELP! inoculation and PF Tek [Re: Inocuole]
#22419171 - 10/22/15 05:07 PM (8 years, 3 months ago) |
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It's officially been 5 full days since inoculation and there's no sign of mycelium growth on all but 1. One the 1, there's a little patch of green/blue. Mold? Already? Do you guys think the needle swap and lack of development is related?
Before swapping the needles out, I boiled water and sucked it up and injected it out of the old needle several times. Then flamed it before every injection.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
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Re: HELP! inoculation and PF Tek [Re: dynamitehack]
#22419190 - 10/22/15 05:13 PM (8 years, 3 months ago) |
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Sounds like that one contam'd since it would be very odd to be getting any bruising so early on before seeing any white.
Hopefully you had better luck with the others. 2 days to 3 weeks for spore germination. Be patient.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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