|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
why poo?
#22394625 - 10/17/15 06:13 PM (8 years, 3 months ago) |
|
|
inquiring minds want to know >.>
out of all the things you can choose to grow shrooms off of why choose shit?
simply knowing that fact would make me not want to eat them
this reminds me too, as an aside
i have a theory - you know how truffle spores are spread underground by mole feces?
maybe horses or cows like to trip
then they would eat the shrooms and poop out the more viable spores
they would happy in their new home :3
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
tetherface
get in where you fit in



Registered: 10/05/14
Posts: 553
Loc: wild
Last seen: 6 years, 6 months
|
Re: why poo? [Re: micro]
#22394644 - 10/17/15 06:18 PM (8 years, 3 months ago) |
|
|
I always wondered if the animals get high like we do? my outdoor patch was plagued by slugs and them little bastards could EAT! to me I don't see why they wouldn't trip at least the first few meals till tolerance kicks in
Edited by tetherface (10/17/15 06:19 PM)
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: why poo? [Re: micro]
#22394649 - 10/17/15 06:19 PM (8 years, 3 months ago) |
|
|
Well, nature chose poo as the substrate to grow mushrooms. So why not replicate it as best as possible? Just so you know, most of the guys here (not me) use coir these days.
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: why poo? [Re: azur]
#22394667 - 10/17/15 06:24 PM (8 years, 3 months ago) |
|
|
lsd affects spiders so i don't see why psilocybin necessarily wouldn't effect other animals
re: replicating nature -- because nature is cruel :V
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
|
Re: why poo? [Re: azur] 1
#22394672 - 10/17/15 06:26 PM (8 years, 3 months ago) |
|
|
Terence mckenna once told a story about raccoons finding his colonized grain jars. They allegedly opened up the jars and ate the grains, and could be heard making quite the racket later on.
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
|
haha, i bet
raccoons are smart, too
i bet they tried to come back later on and get more :V
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
|
Re: why poo? [Re: micro]
#22394701 - 10/17/15 06:35 PM (8 years, 3 months ago) |
|
|
Dung lovers...
--------------------
Q&A US vs. THEM The more I learn, the less I know.
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: why poo? [Re: LocN9ne]
#22394728 - 10/17/15 06:40 PM (8 years, 3 months ago) |
|
|
is there any huge difference between that vs. other substrates though?
in nature, mushrooms have a niche, i get that
indoor cultivation is not always the same
ime, parboiled rice works great by itself
no poo involved and you know this, and can find comfort in that fact :V
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
|
Re: why poo? [Re: micro]
#22394736 - 10/17/15 06:43 PM (8 years, 3 months ago) |
|
|
Shit doesn't bother me one bit... but I'm a nasty motherfucker
--------------------
Q&A US vs. THEM The more I learn, the less I know.
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: why poo? [Re: LocN9ne]
#22394758 - 10/17/15 06:49 PM (8 years, 3 months ago) |
|
|
well, i know at least one coprophile >.>
not you; "doesn't bother" is not the same as "s sexually aroused by"
this person to which i am referring is kind of a nasty one though
like... right when we met he proceeded to tell me that and a whole bunch of other crap i didn't want to know
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: why poo? [Re: LocN9ne]
#22394774 - 10/17/15 06:54 PM (8 years, 3 months ago) |
|
|
Shit doesn't bother me one bit... but I'm the nastiest motherfucker
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: why poo? [Re: azur]
#22394790 - 10/17/15 06:57 PM (8 years, 3 months ago) |
|
|
Quote:
azur said: boner cloner of otd

-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: why poo? [Re: micro]
#22394796 - 10/17/15 06:58 PM (8 years, 3 months ago) |
|
|
So break down your cultivation process for us from start to finish if you would.
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: why poo? [Re: azur]
#22394825 - 10/17/15 07:04 PM (8 years, 3 months ago) |
|
|
hah, okay this will take a bit but my friend hasn't gotten back to me anyway so I'll give it a shot
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: why poo? [Re: micro]
#22394828 - 10/17/15 07:05 PM (8 years, 3 months ago) |
|
|
Cool. Looking forward to it.
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: why poo? [Re: azur]
#22394930 - 10/17/15 07:28 PM (8 years, 3 months ago) |
|
|
poo is free.
pasteurization is cheaper than sterilization.
besides personal theories, those would be my two arguments.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
|
Re: why poo? [Re: micro]
#22394956 - 10/17/15 07:35 PM (8 years, 3 months ago) |
|
|
Well, this is what I've found works the best:
Materials:
All American Pressure Cooker Autoclavable bags with self-healing inoculation port and .2um filter patches Heat sealer Autoclavable bottles with septum lids Heat sealer Glass syringes (these are reusable and autoclavable) Parboiled rice (cheap as hell in any grocery store) Spore syringes (you can go by prints as well but it is up to you to ensure sterility) Plastic bins and either saran wrap or plastic sheeting, depending on the size of the bins Peat and lime (to make casing, a la Stamets et al.) Heater Paper clips Rubbing alcohol (preferably ethanol) either 70% or 91% Spore syringes egular commercial bleach
Optional materials:
Bale(s) of straw Materials to make liquid media (I stole LB media from the lab I worked at but potato dextrose broth is better anyway) Lots of plastic sheeting, staple gun, duct tape, mini hepa filter for room Autoclave tape (useful when sterilizing liquids) Bunsen burner Glass petri dishes (or sterile disposable plastic ones)
Protocol:
Cook some parboiled rice using 3/4 of the water listed in the directions. The parboiled rice is precooked a bit and it comes out less sticky and more airy than brown rice Put a liberal amount in the bags but not so much it is filled more than a third of the way up to the filter patch Gently fold the top of the bag 1/2" down 4 or 5 times after letting the air out and secure them that way with a paper clip on each end Sterilize for 90 minutes at 15 PSI (make sure it is there before counting) Always let the PC cool before opening it up Open it up and heat seal the bags close. You will need to adjust the heat sealer so it won't burn the bag apart so practice with a test bag first a few times until you get the setting right Once the bags all have a GOOD seal (if they split open they are unusable) then let them cool to room temperature on the inside as well as the outside Clean a room with 2% commercial bleach (probably should do this beforehand; we always brought the pressure cooker into a cleaned room before opening it and heat sealed it in there) Optionally the clean room can be lined with the plastic sheeting (see the positive pressure flow room link in my sig and the optional materials applicable) In the clean room, with either clean gloves or scrubbed hands, wipe down the inoculation site with your ethanol and then knock it up with the spore syringe With the bunsen burner (optional) or a flame, sterilize the syringe and replace the cap and let it cool before knocking up the next bag. think we only used 1 or 2 cc's per bag; you don't need a lot Colonize he bags at 80F or so. I was really ghetto about this and used a space heater in a closet and opened the door a certain amount to control the heat Let the bags colonize to about 15% and then break it apart and mix around the mycelium so it will colonize exponentially faster
--- and shoot ---
I gotta take off >.<
But I'll finish this later on and break it out into a new thread
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: why poo? [Re: micro]
#22395034 - 10/17/15 07:50 PM (8 years, 3 months ago) |
|
|
Quote:
micro said: sterilize the syringe and replace the cap and let it cool before knocking up the next bag.
Colonize he bags at 80F or so.
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: why poo? [Re: micro]
#22395099 - 10/17/15 08:04 PM (8 years, 3 months ago) |
|
|
parboiled rice. I'm interested in this.
|
Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
|
Re: why poo? [Re: azur]
#22395155 - 10/17/15 08:13 PM (8 years, 3 months ago) |
|
|
Quote:
azur said: parboiled rice. I'm interested in this.

Might just have to give it a shot
|
|