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Invisiblemicro
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why poo?
    #22394625 - 10/17/15 06:13 PM (8 years, 3 months ago)

inquiring minds want to know >.>

out of all the things you can choose to grow shrooms off of why choose shit?

simply knowing that fact would make me not want to eat them

this reminds me too, as an aside

i have a theory - you know how truffle spores are spread underground by mole feces?

maybe horses or cows like to trip

then they would eat the shrooms and poop out the more viable spores

they would happy in their new home :3


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Offlinetetherface
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Re: why poo? [Re: micro]
    #22394644 - 10/17/15 06:18 PM (8 years, 3 months ago)

I always wondered if the animals get high like we do? my outdoor patch was plagued by slugs and them little bastards could EAT! to me I don't see why they wouldn't trip at least the first few meals till tolerance kicks in:shrug:


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Edited by tetherface (10/17/15 06:19 PM)


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Invisibleazur
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Re: why poo? [Re: micro]
    #22394649 - 10/17/15 06:19 PM (8 years, 3 months ago)

Well, nature chose poo as the substrate to grow mushrooms. So why not replicate it as best as possible?
Just so you know, most of the guys here (not me) use coir these days.


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A cube is NOT a cube.

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Invisiblemicro
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Re: why poo? [Re: azur]
    #22394667 - 10/17/15 06:24 PM (8 years, 3 months ago)

lsd affects spiders so i don't see why psilocybin necessarily wouldn't effect other animals

re: replicating nature -- because nature is cruel :V


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OfflinePsilosopherr
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Re: why poo? [Re: azur] * 1
    #22394672 - 10/17/15 06:26 PM (8 years, 3 months ago)

Terence mckenna once told a story about raccoons finding his colonized grain jars. They allegedly opened up the jars and ate the grains, and could be heard making quite the racket later on.


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Invisiblemicro
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Re: why poo? [Re: Psilosopherr]
    #22394678 - 10/17/15 06:28 PM (8 years, 3 months ago)

haha, i bet

raccoons are smart, too

i bet they tried to come back later on and get more :V


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InvisibleLocN9ne
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Re: why poo? [Re: micro]
    #22394701 - 10/17/15 06:35 PM (8 years, 3 months ago)

Dung lovers...


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Invisiblemicro
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Re: why poo? [Re: LocN9ne]
    #22394728 - 10/17/15 06:40 PM (8 years, 3 months ago)

is there any huge difference between that vs. other substrates though?

in nature, mushrooms have a niche, i get that

indoor cultivation is not always the same

ime, parboiled rice works great by itself

no poo involved and you know this, and can find comfort in that fact :V


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InvisibleLocN9ne
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Re: why poo? [Re: micro]
    #22394736 - 10/17/15 06:43 PM (8 years, 3 months ago)

Shit doesn't bother me one bit... but I'm a nasty motherfucker


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Invisiblemicro
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Re: why poo? [Re: LocN9ne]
    #22394758 - 10/17/15 06:49 PM (8 years, 3 months ago)

well, i know at least one coprophile >.>

not you; "doesn't bother" is not the same as "s sexually aroused by"

this person to which i am referring is kind of a nasty one though

like... right when we met he proceeded to tell me that and a whole bunch of other crap i didn't want to know


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Invisibleazur
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Re: why poo? [Re: LocN9ne]
    #22394774 - 10/17/15 06:54 PM (8 years, 3 months ago)

Shit doesn't bother me one bit... but I'm the nastiest motherfucker


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FALL IN LOVE WITH LC
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Invisiblemicro
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Re: why poo? [Re: azur]
    #22394790 - 10/17/15 06:57 PM (8 years, 3 months ago)

Quote:

azur said:
boner cloner of otd




:thumbup: :thumbup: :thumbup:

:awesomenod:


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Invisibleazur
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Re: why poo? [Re: micro]
    #22394796 - 10/17/15 06:58 PM (8 years, 3 months ago)

So break down your cultivation process for us from start to finish if you would.


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FALL IN LOVE WITH LC
FOTTSE!!!
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Invisiblemicro
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Re: why poo? [Re: azur]
    #22394825 - 10/17/15 07:04 PM (8 years, 3 months ago)

hah, okay this will take a bit but my friend hasn't gotten back to me anyway so I'll give it a shot


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Invisibleazur
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Re: why poo? [Re: micro]
    #22394828 - 10/17/15 07:05 PM (8 years, 3 months ago)

Cool. Looking forward to it.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
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Invisibleblindingleaf
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Re: why poo? [Re: azur]
    #22394930 - 10/17/15 07:28 PM (8 years, 3 months ago)

poo is free.

pasteurization is cheaper than sterilization.

besides personal theories, those would be my two arguments.


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Invisiblemicro
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Re: why poo? [Re: micro]
    #22394956 - 10/17/15 07:35 PM (8 years, 3 months ago)

Well, this is what I've found works the best:

Materials:

All American Pressure Cooker
Autoclavable bags with self-healing inoculation port and .2um filter patches
Heat sealer
Autoclavable bottles with septum lids
Heat sealer
Glass syringes (these are reusable and autoclavable)
Parboiled rice (cheap as hell in any grocery store)
Spore syringes (you can go by prints as well but it is up to you to ensure sterility)
Plastic bins and either saran wrap or plastic sheeting, depending on the size of the bins
Peat and lime (to make casing, a la Stamets et al.)
Heater
Paper clips
Rubbing alcohol (preferably ethanol) either 70% or 91%
Spore syringes
egular commercial bleach

Optional materials:

Bale(s) of straw
Materials to make liquid media (I stole LB media from the lab I worked at but potato dextrose broth is better anyway)
Lots of plastic sheeting, staple gun, duct tape, mini hepa filter for room
Autoclave tape (useful when sterilizing liquids)
Bunsen burner
Glass petri dishes (or sterile disposable plastic ones)


Protocol:

Cook some parboiled rice using 3/4 of the water listed in the directions. The parboiled rice is precooked a bit and it comes out less sticky and more airy than brown rice
Put a liberal amount in the bags but not so much it is filled more than a third of the way up to the filter patch
Gently fold the top of the bag 1/2" down 4 or 5 times after letting the air out and secure them that way with a paper clip on each end
Sterilize for 90 minutes at 15 PSI (make sure it is there before counting)
Always let the PC cool before opening it up
Open it up and heat seal the bags close.  You will need to adjust the heat sealer so it won't burn the bag apart so practice with a test bag first a few times until you get the setting right
Once the bags all have a GOOD seal (if they split open they are unusable) then let them cool to room temperature on the inside as well as the outside
Clean a room with 2% commercial bleach (probably should do this beforehand; we always brought the pressure cooker into a cleaned room before opening it and heat sealed it in there)
Optionally the clean room can be lined with the plastic sheeting (see the positive pressure flow room link in my sig and the optional materials applicable)
In the clean room, with either clean gloves or scrubbed hands, wipe down the inoculation site with your ethanol and then knock it up with the spore syringe
With the bunsen burner (optional) or a flame, sterilize the syringe and replace the cap and let it cool before knocking up the next bag.  think we only used 1 or 2 cc's per bag; you don't need a lot
Colonize he bags at 80F or so. I was really ghetto about this and used a space heater in a closet and opened the door a certain amount to control the heat
Let the bags colonize to about 15% and then break it apart and mix around the mycelium so it will colonize exponentially faster




--- and shoot ---

I gotta take off >.<

But I'll finish this later on and break it out into a new thread


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InvisibleInocuole
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Re: why poo? [Re: micro]
    #22395034 - 10/17/15 07:50 PM (8 years, 3 months ago)

Quote:

micro said:
sterilize the syringe and replace the cap and let it cool before knocking up the next bag.

Colonize he bags at 80F or so.




:thatsinteresting:


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Invisibleazur
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Re: why poo? [Re: micro]
    #22395099 - 10/17/15 08:04 PM (8 years, 3 months ago)

parboiled rice. I'm interested in this.


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FALL IN LOVE WITH LC
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OfflinePsilosopherr
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Re: why poo? [Re: azur]
    #22395155 - 10/17/15 08:13 PM (8 years, 3 months ago)

Quote:

azur said:
parboiled rice. I'm interested in this.



:whathesaid:

Might just have to give it a shot


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