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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Growing shrooms from spore print?
#22389394 - 10/16/15 05:03 PM (8 years, 3 months ago) |
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Planning on doing a BRF/PF tek. After my mushrooms grow I want to take spore prints due to little money and not having any syringes so I'm wondering, if I just have a spore print on some paper, how difficult is it to grow some more shrooms with just the spore print? I know I'll have a higher chance of contamination but any helpful advice is appreciated.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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thelivingfreekshow
Fuck You



Registered: 02/07/11
Posts: 2,043
Loc: Prifddinas, Gielinor
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22389407 - 10/16/15 05:07 PM (8 years, 3 months ago) |
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It can be done, if your sterile technique is good enough, but there are a few easy agar teks you could use to make sure you get a clean start
like this one: http://www.shroomery.org/forums/showflat.php/Number/19208976
Also, dont take prints on paper, thats a bad idea.
Edited by thelivingfreekshow (10/16/15 05:11 PM)
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22389410 - 10/16/15 05:08 PM (8 years, 3 months ago) |
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All spore syringes start from print. You can make a syringe clean enough for PF tel relatively easy with no special equipment if you learn sterile techniques and how to properly utilize a still air box.
Of course if you were willing to step it up a notch you could put those spores to agar plates, and go from there. But you will probably want a pressure cooker. Anything past using a spore syringe for BRF cakes you will need a pressure cooker and definitely want to clean your culture up on agar
Don't put your spores to paper, use a fresh piece of a lluminum foil. Check out RR letsgrowmushrooms videos for a great chapter on spore printing and syringe making at home
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (10/16/15 05:10 PM)
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
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Last seen: 3 months, 20 days
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22389415 - 10/16/15 05:10 PM (8 years, 3 months ago) |
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Generally you would want to print onto clean foil or into a steam sterilized jar or container. Do it in still air conditions with sanitized gloves, be as fast as possible, leave it open for the shortest amount of time possible.
Idealy, you would do this with an Invitro grow in a still air box to get very clean prints. There are plenty of guides for spore printing on this site.
The chances of contams will be higher, but at the same time it's definitely not hard to be successful growing from open air prints.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Wow thanks for the quick replies guys 
I should probably note that I do have a pressure cooker
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22390041 - 10/16/15 07:25 PM (8 years, 3 months ago) |
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Oh well then Right on man if you have a PC and have a print then you have the option of moving beyond PF tek, using agar to create clean innoculant and making clean grain spawn
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 Amateur Mycologists United AMU Q&A
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: mushpunx]
#22390312 - 10/16/15 08:18 PM (8 years, 3 months ago) |
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Quote:
mushpunx said: Oh well then Right on man if you have a PC and have a print then you have the option of moving beyond PF tek, using agar to create clean innoculant and making clean grain spawn 
I heard to do grain to grain you had to have like some badass air filter in the room and a ridiculously sterile environment though :/ It's my first time ever growing so I'm not really educated on all of this that much tbh. I ordered a spore syringe, it's just that after I grow those mushrooms from that syringe I won't have any money to do much else other than take spore prints. Would I be able to re-use that syringe that I ordered by any chance?
P.S. this is the strain I ordered. They just made my mouth water when I saw them and I was like "Yep, that's the one."
Huautla Psilocybe Cubensis
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22390329 - 10/16/15 08:21 PM (8 years, 3 months ago) |
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Also, very random question, but I should pick them while the caps are still attatched to the stems right? They're more potent before they have a chance to show their spores aren't they? Hopefully that makes sense
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22390395 - 10/16/15 08:34 PM (8 years, 3 months ago) |
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Yes you can reuse a syringe, there's a few teks for that... as for harvesting...when the veil is breaking is a good time to pick to avoid a sporesplosion
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Q&A US vs. THEM The more I learn, the less I know.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: LocN9ne]
#22390415 - 10/16/15 08:37 PM (8 years, 3 months ago) |
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Quote:
LocN9ne said: Yes you can reuse a syringe, there's a few teks for that... as for harvesting...when the veil is breaking is a good time to pick to avoid a sporesplosion
Thanks for answering my noob questions c: I'll do more research when it gets closer to time, I'm just really tired today and thought it'd be easier to make a couple of posts. My brain needs a rest from the search bar
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22391137 - 10/16/15 10:36 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said:
Quote:
mushpunx said: Oh well then Right on man if you have a PC and have a print then you have the option of moving beyond PF tek, using agar to create clean innoculant and making clean grain spawn 
I heard to do grain to grain you had to have like some badass air filter in the room and a ridiculously sterile environment though :/ It's my first time ever growing so I'm not really educated on all of this that much tbh. I ordered a spore syringe, it's just that after I grow those mushrooms from that syringe I won't have any money to do much else other than take spore prints. Would I be able to re-use that syringe that I ordered by any chance?
P.S. this is the strain I ordered. They just made my mouth water when I saw them and I was like "Yep, that's the one."
Huautla Psilocybe Cubensis

I've done Grain to Grain many many times now...All you need is a SAB, good sterile techniques, and of course the Master and receiving jars. Grain to Grain is a great way to expand your Spawn!
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
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Re: Growing shrooms from spore print? [Re: Darkhome]
#22391240 - 10/16/15 11:02 PM (8 years, 3 months ago) |
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print to agar- agar to clean transfer- clean plate to whatever you want
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: Growing shrooms from spore print? [Re: spirit_shadow]
#22394372 - 10/17/15 05:14 PM (8 years, 3 months ago) |
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Yea you are thinking of a laminar flow hood, like the one in my sig.
It pushes air through a HEPA filter at a special rate, so you have sterile air in laminar flow, giving you a certain amount of space to work in front of the hood. The air coming out is sterile, but what's important is the laminar flow. It makes it so you can keep a sterile container sterile with the lid off.
A flow hood is super awesome and convenient but all it really does is give you elbow room. You can accomplish anything you would do in front of a hood in a still air box.
A still air box isn't sterile, it doesn't have to be. All it does is keep the air still so you can keep contaminants from landing in your work.
Both require practice and tip top sterile technique.
If you have a pressure cooker and still air box there is no limit to what you can grow at home!
--------------------
 Amateur Mycologists United AMU Q&A
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: mushpunx]
#22394623 - 10/17/15 06:12 PM (8 years, 3 months ago) |
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My god you guys are motivating the crap out of me. I can make unlimited shrooms if I have a pressure cooker and an SAB? Am I dreaming? I feel like I'm losing my virginity all over again (hopefully with more success though) Thank you all so much, I'll be sure to come back and let you guys know how my grow goes!
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22394718 - 10/17/15 06:39 PM (8 years, 3 months ago) |
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With a pressure cooker, a sab, and sterile agar techniques...one could grow more mushrooms than one could eat from a single drop from a spore syringe...get to work boy
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Q&A US vs. THEM The more I learn, the less I know.
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: LocN9ne]
#22395024 - 10/17/15 07:48 PM (8 years, 3 months ago) |
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after looking at the agar teks my head hurts. I'm having a hard time even understanding the easy teks I've been reading a lot today and staring at my computer screen a lot too so that probably doesn't help...
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22395094 - 10/17/15 08:03 PM (8 years, 3 months ago) |
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Can you make jello? Do you own a PC? Do you shower regularly? If so, you can succeed at agar... stop being scared.
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Q&A US vs. THEM The more I learn, the less I know.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Growing shrooms from spore print? [Re: LocN9ne]
#22395217 - 10/17/15 08:22 PM (8 years, 3 months ago) |
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Quote:
Mad Season said:
agar is just as easy to do as LC. It's the exact same thing really. Whatever your recipe was, you can just add 2g of agar for every 100 ml.
Quote:
Mad Season said:
Quote:
hiperhiper said: so what's the right procedure with grains? I've checked the teks and nobody said anything about this. I didn't know I was not supposed to use syringe on grains. What is cleaning up syringe on agar? what does it mean?
http://www.shroomery.org/forums/showflat.php/Number/18431006 http://www.shroomery.org/forums/showflat.php/Number/18430998 http://www.shroomery.org/forums/showflat.php/Number/19208976 http://www.shroomery.org/forums/showflat.php/Number/21889950/page/1
All of those threads are amazing.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22395260 - 10/17/15 08:32 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said: after looking at the agar teks my head hurts. I'm having a hard time even understanding the easy teks I've been reading a lot today and staring at my computer screen a lot too so that probably doesn't help...
Try watching letsgrowmushrooms by RR, great section on agar, pouring plates and culture work. Easier with a visual aid then read up
--------------------
 Amateur Mycologists United AMU Q&A
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Growing shrooms from spore print? [Re: mushpunx]
#22395264 - 10/17/15 08:33 PM (8 years, 3 months ago) |
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I might have to watch those videos one day
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Growing shrooms from spore print? [Re: Mad Season]
#22395289 - 10/17/15 08:39 PM (8 years, 3 months ago) |
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Quote:
Mad Season said:
I might have to watch those videos one day
Don't waste your time...he doesn't even keep all his projects in complete darkness like your supposed to...
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Q&A US vs. THEM The more I learn, the less I know.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
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Re: Growing shrooms from spore print? [Re: LocN9ne]
#22395323 - 10/17/15 08:45 PM (8 years, 3 months ago) |
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Hahaha is that a joke?
You should dude. I still refer to them sometimes. I learned everything from doing my own research but once I saw the videos I was like...dude.. could have saved some time haha
--------------------
 Amateur Mycologists United AMU Q&A
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: mushpunx]
#22398323 - 10/18/15 03:28 PM (8 years, 3 months ago) |
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Okay so the "Let's Grow Mushrooms!" video on agar made things a lot more understandable as far as setting up the agar and getting it into the petri dishes goes....but I'm still not sure how you inoculate your spores into the agar?
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 3 hours, 13 minutes
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22398691 - 10/18/15 04:49 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said: Okay so the "Let's Grow Mushrooms!" video on agar made things a lot more understandable as far as setting up the agar and getting it into the petri dishes goes....but I'm still not sure how you inoculate your spores into the agar?
dude, you should look into pastywhites agar tek. its simple, cheap, and works 
Edit: http://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 in case you are feeling lazy :p
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
Edited by spirit_shadow (10/18/15 04:52 PM)
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thelivingfreekshow
Fuck You



Registered: 02/07/11
Posts: 2,043
Loc: Prifddinas, Gielinor
Last seen: 5 years, 1 month
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Quote:
thelivingfreekshow said: It can be done, if your sterile technique is good enough, but there are a few easy agar teks you could use to make sure you get a clean start
like this one: http://www.shroomery.org/forums/showflat.php/Number/19208976
Also, dont take prints on paper, thats a bad idea.
His tek is in the link above,very simple
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Ok but he's asking how to inoculate. Not how to do agar. Easy agar is a great tek tho.
http://www.shroomery.org/forums/showflat.php/Number/21889950
This is an easy way
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: Mad Season]
#22398871 - 10/18/15 05:28 PM (8 years, 3 months ago) |
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Quote:
Mad Season said: Ok but he's asking how to inoculate. Not how to do agar. Easy agar is a great tek tho.
http://www.shroomery.org/forums/showflat.php/Number/21889950
This is an easy way
YES! This is exactly what I was looking for, thank you so much. That answered almost everything for me really quickly.
The last question I have, in the "Let's Grow Mushrooms" video the guy said after he took the time to run around and get his agar mixture stuff (bar of agar-agar, potato flakes, honey, etc.) he found that it was easier and about the same price to just buy a powder that was already made? or something along those lines... Can anyone happen to link me to such a powder or does everyone here just make it from the bar of agar-agar?
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22399335 - 10/18/15 06:45 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said: Can anyone happen to link me to such a powder or does everyone here just make it from the bar of agar-agar?
You can get this agar agar powder from ebay or an asian food market/ health food store. Never used the bar of agar before. Got a pack of 3 for like 6 bucks + free shipping. It should last me a while.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Re: Growing shrooms from spore print? [Re: Psilosoulful]
#22399546 - 10/18/15 07:16 PM (8 years, 3 months ago) |
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inoculation is simple whether you poured your plates or use no pour pasty plates.
There is a section in letsgrowmushrooms on how to inoculate agar from spore print, maybe you it idnt watch?
Its simple. In your still air box open your print. Flame sterilize a wire loop and cool it in your blank agar plate, then swipe a small circle on your print, then swipe your plate. Easy peasy. Innoculating from a spore syringe then flame sterilize syringe and drop onto a sterile swab or loop and swipe plate. Alternatively you can drip directly onto your blank plate but it slides around and makes a mess of germination
--------------------
 Amateur Mycologists United AMU Q&A
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: mushpunx]
#22400225 - 10/18/15 08:52 PM (8 years, 3 months ago) |
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Quote:
Psilosoulful said:
Quote:
Kush_Zombie said: Can anyone happen to link me to such a powder or does everyone here just make it from the bar of agar-agar?
You can get this agar agar powder from ebay or an asian food market/ health food store. Never used the bar of agar before. Got a pack of 3 for like 6 bucks + free shipping. It should last me a while.

Would you still need to mix in the potato flakes and honey?
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mushpunx said: inoculation is simple whether you poured your plates or use no pour pasty plates.
There is a section in letsgrowmushrooms on how to inoculate agar from spore print, maybe you it idnt watch?
Its simple. In your still air box open your print. Flame sterilize a wire loop and cool it in your blank agar plate, then swipe a small circle on your print, then swipe your plate. Easy peasy. Innoculating from a spore syringe then flame sterilize syringe and drop onto a sterile swab or loop and swipe plate. Alternatively you can drip directly onto your blank plate but it slides around and makes a mess of germination
I searched for the video but I had difficult finding it :/ Mad Season posted a tek that was extremely helpful though
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 11 hours
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22400284 - 10/18/15 09:04 PM (8 years, 3 months ago) |
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You have to buy them. 8 bux but worth every penny. Just google lets grow mushrooms and buy the downloads
Yes you would have to mix in nutrients with the agar agar. I use telephone brand myself.
For PDA I use 10g flakes 10g agar couple drops honey or Karo syrup and 500ml water.
I prefer MEA, I buy malt extract from brewers store. Cooks up clearer for me. Same proportions except you don't add any honey.
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 Amateur Mycologists United AMU Q&A
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22400290 - 10/18/15 09:04 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said:
Quote:
Psilosoulful said:
Quote:
Kush_Zombie said: Can anyone happen to link me to such a powder or does everyone here just make it from the bar of agar-agar?
You can get this agar agar powder from ebay or an asian food market/ health food store. Never used the bar of agar before. Got a pack of 3 for like 6 bucks + free shipping. It should last me a while.

Would you still need to mix in the potato flakes and honey?
Yes, I use Munchauzen's recipe. Here's his agar prep video!
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: Psilosoulful]
#22400383 - 10/18/15 09:23 PM (8 years, 3 months ago) |
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Thank you all so much for taking the time to help me understand all of this. I really appreciate it.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22400399 - 10/18/15 09:26 PM (8 years, 3 months ago) |
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No problem dude!
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 3 hours, 13 minutes
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22400591 - 10/18/15 10:28 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said: Thank you all so much for taking the time to help me understand all of this. I really appreciate it.
Thats just how we do here in the M.C.
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Growing shrooms from spore print? [Re: spirit_shadow]
#22400601 - 10/18/15 10:30 PM (8 years, 3 months ago) |
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Quote:
spirit_shadow said:
Quote:
Kush_Zombie said: Thank you all so much for taking the time to help me understand all of this. I really appreciate it.
Thats just how we do here in the M.C. 
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: Growing shrooms from spore print? [Re: Psilosoulful]
#22400878 - 10/19/15 12:27 AM (8 years, 3 months ago) |
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Highly recommend grainwater agar. That's just the boil water left from your grain prep. Strain your shit over another pot and save it. It's enough to make enough agar plates for months.
All you do is use 1/8th tsp agar powder and 25ml of grainwater per plate instead of all that karo potato bla bla. If the grainwater is really rich, you may want to dilute it, since it can promote slower tometose growth.
I think violet, munchauzen, and probably hamloaf have teks on that. You can use it in minirounds or whatever PP5 container you can find. I use hummus containers from the dollarstore that are 5 for $1.
By the way, incase you haven't read or understood, there are two ways to do agar:
No-pour means you pour an appropriate amount of agar powder + nutrients + water mix into individual containers, pressure cook them, then they congeal when they cool down to room temperature.
Pour agar means you make a large mix of agar powder and nutrient water in a bottle which is then PC'd and removed while hot. While still hot, this still liquid mix is poured into sterile petris or some other sterile container in a SAB or LFH , where is then congeals.
No-pour is better if you don't have your sterile technique down. Pour is better if you want to produce a shitton of plates without a shitton of PC runs or pricier containers. That said, the petris cannot be resterilized. If you use space efficient nopour containers, you can jam a lot in the PC. With my dollarstore containers, I can fit ~20 in my 23qt PC, and they're reusable. Reusable containers for pour agar will need to be sterilized anyway, so why not put the agar in while you do it and save the hastle of pouring.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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