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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: Growing shrooms from spore print? [Re: Mad Season]
#22395289 - 10/17/15 08:39 PM (8 years, 3 months ago) |
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Quote:
Mad Season said:
I might have to watch those videos one day
Don't waste your time...he doesn't even keep all his projects in complete darkness like your supposed to...
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Q&A US vs. THEM The more I learn, the less I know.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Re: Growing shrooms from spore print? [Re: LocN9ne]
#22395323 - 10/17/15 08:45 PM (8 years, 3 months ago) |
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Hahaha is that a joke?
You should dude. I still refer to them sometimes. I learned everything from doing my own research but once I saw the videos I was like...dude.. could have saved some time haha
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 Amateur Mycologists United AMU Q&A
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: mushpunx]
#22398323 - 10/18/15 03:28 PM (8 years, 3 months ago) |
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Okay so the "Let's Grow Mushrooms!" video on agar made things a lot more understandable as far as setting up the agar and getting it into the petri dishes goes....but I'm still not sure how you inoculate your spores into the agar?
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 1 hour, 5 minutes
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22398691 - 10/18/15 04:49 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said: Okay so the "Let's Grow Mushrooms!" video on agar made things a lot more understandable as far as setting up the agar and getting it into the petri dishes goes....but I'm still not sure how you inoculate your spores into the agar?
dude, you should look into pastywhites agar tek. its simple, cheap, and works 
Edit: http://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 in case you are feeling lazy :p
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
Edited by spirit_shadow (10/18/15 04:52 PM)
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thelivingfreekshow
Fuck You



Registered: 02/07/11
Posts: 2,043
Loc: Prifddinas, Gielinor
Last seen: 5 years, 1 month
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Quote:
thelivingfreekshow said: It can be done, if your sterile technique is good enough, but there are a few easy agar teks you could use to make sure you get a clean start
like this one: http://www.shroomery.org/forums/showflat.php/Number/19208976
Also, dont take prints on paper, thats a bad idea.
His tek is in the link above,very simple
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Ok but he's asking how to inoculate. Not how to do agar. Easy agar is a great tek tho.
http://www.shroomery.org/forums/showflat.php/Number/21889950
This is an easy way
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: Mad Season]
#22398871 - 10/18/15 05:28 PM (8 years, 3 months ago) |
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Quote:
Mad Season said: Ok but he's asking how to inoculate. Not how to do agar. Easy agar is a great tek tho.
http://www.shroomery.org/forums/showflat.php/Number/21889950
This is an easy way
YES! This is exactly what I was looking for, thank you so much. That answered almost everything for me really quickly.
The last question I have, in the "Let's Grow Mushrooms" video the guy said after he took the time to run around and get his agar mixture stuff (bar of agar-agar, potato flakes, honey, etc.) he found that it was easier and about the same price to just buy a powder that was already made? or something along those lines... Can anyone happen to link me to such a powder or does everyone here just make it from the bar of agar-agar?
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22399335 - 10/18/15 06:45 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said: Can anyone happen to link me to such a powder or does everyone here just make it from the bar of agar-agar?
You can get this agar agar powder from ebay or an asian food market/ health food store. Never used the bar of agar before. Got a pack of 3 for like 6 bucks + free shipping. It should last me a while.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Re: Growing shrooms from spore print? [Re: Psilosoulful]
#22399546 - 10/18/15 07:16 PM (8 years, 3 months ago) |
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inoculation is simple whether you poured your plates or use no pour pasty plates.
There is a section in letsgrowmushrooms on how to inoculate agar from spore print, maybe you it idnt watch?
Its simple. In your still air box open your print. Flame sterilize a wire loop and cool it in your blank agar plate, then swipe a small circle on your print, then swipe your plate. Easy peasy. Innoculating from a spore syringe then flame sterilize syringe and drop onto a sterile swab or loop and swipe plate. Alternatively you can drip directly onto your blank plate but it slides around and makes a mess of germination
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 Amateur Mycologists United AMU Q&A
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: mushpunx]
#22400225 - 10/18/15 08:52 PM (8 years, 3 months ago) |
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Quote:
Psilosoulful said:
Quote:
Kush_Zombie said: Can anyone happen to link me to such a powder or does everyone here just make it from the bar of agar-agar?
You can get this agar agar powder from ebay or an asian food market/ health food store. Never used the bar of agar before. Got a pack of 3 for like 6 bucks + free shipping. It should last me a while.

Would you still need to mix in the potato flakes and honey?
Quote:
mushpunx said: inoculation is simple whether you poured your plates or use no pour pasty plates.
There is a section in letsgrowmushrooms on how to inoculate agar from spore print, maybe you it idnt watch?
Its simple. In your still air box open your print. Flame sterilize a wire loop and cool it in your blank agar plate, then swipe a small circle on your print, then swipe your plate. Easy peasy. Innoculating from a spore syringe then flame sterilize syringe and drop onto a sterile swab or loop and swipe plate. Alternatively you can drip directly onto your blank plate but it slides around and makes a mess of germination
I searched for the video but I had difficult finding it :/ Mad Season posted a tek that was extremely helpful though
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22400284 - 10/18/15 09:04 PM (8 years, 3 months ago) |
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You have to buy them. 8 bux but worth every penny. Just google lets grow mushrooms and buy the downloads
Yes you would have to mix in nutrients with the agar agar. I use telephone brand myself.
For PDA I use 10g flakes 10g agar couple drops honey or Karo syrup and 500ml water.
I prefer MEA, I buy malt extract from brewers store. Cooks up clearer for me. Same proportions except you don't add any honey.
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 Amateur Mycologists United AMU Q&A
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22400290 - 10/18/15 09:04 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said:
Quote:
Psilosoulful said:
Quote:
Kush_Zombie said: Can anyone happen to link me to such a powder or does everyone here just make it from the bar of agar-agar?
You can get this agar agar powder from ebay or an asian food market/ health food store. Never used the bar of agar before. Got a pack of 3 for like 6 bucks + free shipping. It should last me a while.

Would you still need to mix in the potato flakes and honey?
Yes, I use Munchauzen's recipe. Here's his agar prep video!
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Kush_Zombie
smug piece of shit



Registered: 10/22/14
Posts: 4,793
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Re: Growing shrooms from spore print? [Re: Psilosoulful]
#22400383 - 10/18/15 09:23 PM (8 years, 3 months ago) |
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Thank you all so much for taking the time to help me understand all of this. I really appreciate it.
-------------------- How to get started in bulk: Presto 23-Quart Pressure Cooker BOD's Simple as FUCK Still Air Box PastyWhyte's Easy Agar Tek Munchauzen's Cultivation Video Series How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs) What is G2G? (Grain-to-Grain) Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary) Spitball's Monotub Tek (A liner isn't necessary but is useful) Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22400399 - 10/18/15 09:26 PM (8 years, 3 months ago) |
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No problem dude!
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,674
Last seen: 1 hour, 5 minutes
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
#22400591 - 10/18/15 10:28 PM (8 years, 3 months ago) |
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Quote:
Kush_Zombie said: Thank you all so much for taking the time to help me understand all of this. I really appreciate it.
Thats just how we do here in the M.C.
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Growing shrooms from spore print? [Re: spirit_shadow]
#22400601 - 10/18/15 10:30 PM (8 years, 3 months ago) |
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Quote:
spirit_shadow said:
Quote:
Kush_Zombie said: Thank you all so much for taking the time to help me understand all of this. I really appreciate it.
Thats just how we do here in the M.C. 
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Growing shrooms from spore print? [Re: Psilosoulful]
#22400878 - 10/19/15 12:27 AM (8 years, 3 months ago) |
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Highly recommend grainwater agar. That's just the boil water left from your grain prep. Strain your shit over another pot and save it. It's enough to make enough agar plates for months.
All you do is use 1/8th tsp agar powder and 25ml of grainwater per plate instead of all that karo potato bla bla. If the grainwater is really rich, you may want to dilute it, since it can promote slower tometose growth.
I think violet, munchauzen, and probably hamloaf have teks on that. You can use it in minirounds or whatever PP5 container you can find. I use hummus containers from the dollarstore that are 5 for $1.
By the way, incase you haven't read or understood, there are two ways to do agar:
No-pour means you pour an appropriate amount of agar powder + nutrients + water mix into individual containers, pressure cook them, then they congeal when they cool down to room temperature.
Pour agar means you make a large mix of agar powder and nutrient water in a bottle which is then PC'd and removed while hot. While still hot, this still liquid mix is poured into sterile petris or some other sterile container in a SAB or LFH , where is then congeals.
No-pour is better if you don't have your sterile technique down. Pour is better if you want to produce a shitton of plates without a shitton of PC runs or pricier containers. That said, the petris cannot be resterilized. If you use space efficient nopour containers, you can jam a lot in the PC. With my dollarstore containers, I can fit ~20 in my 23qt PC, and they're reusable. Reusable containers for pour agar will need to be sterilized anyway, so why not put the agar in while you do it and save the hastle of pouring.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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