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InvisibleKush_Zombie
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Registered: 10/22/14
Posts: 4,793
Growing shrooms from spore print?
    #22389394 - 10/16/15 05:03 PM (8 years, 3 months ago)

Planning on doing a BRF/PF tek. After my mushrooms grow I want to take spore prints due to little money and not having any syringes so I'm wondering, if I just have a spore print on some paper, how difficult is it to grow some more shrooms with just the spore print? I know I'll have a higher chance of contamination but any helpful advice is appreciated.


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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Offlinethelivingfreekshow
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22389407 - 10/16/15 05:07 PM (8 years, 3 months ago)

It can be done, if your sterile technique is good enough, but there are a few easy agar teks you could use to make sure you get a clean start

like this one:
http://www.shroomery.org/forums/showflat.php/Number/19208976

Also, dont take prints on paper, thats a bad idea.


Edited by thelivingfreekshow (10/16/15 05:11 PM)


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Offlinemushpunx
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22389410 - 10/16/15 05:08 PM (8 years, 3 months ago)

All spore syringes start from print. You can make a syringe clean enough for PF tel relatively easy with no special equipment if you learn sterile techniques and how to properly utilize a still air box.

Of course if you were willing to step it up a notch you could put those spores to agar plates, and go from there. But you will probably want a pressure cooker.
Anything past using a spore syringe for BRF cakes you will need a pressure cooker and definitely want to clean your culture up on agar

Don't put your spores to paper, use a fresh piece of a lluminum foil. Check out RR letsgrowmushrooms videos for a great chapter on spore printing and syringe making at home

:thumbup:


--------------------

Amateur Mycologists United
AMU Q&A


Edited by mushpunx (10/16/15 05:10 PM)


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OfflineMachiavelliavore
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22389415 - 10/16/15 05:10 PM (8 years, 3 months ago)

Generally you would want to print onto clean foil or into a steam sterilized jar or container.  Do it in still air conditions with sanitized gloves, be as fast as possible, leave it open for the shortest amount of time possible.

Idealy, you would do this with an Invitro grow in a still air box to get very clean prints.  There are plenty of guides for spore printing on this site.


The chances of contams will be higher, but at the same time it's definitely not hard to be successful growing from open air prints.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleKush_Zombie
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Registered: 10/22/14
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Re: Growing shrooms from spore print? [Re: Machiavelliavore]
    #22389436 - 10/16/15 05:14 PM (8 years, 3 months ago)

Wow thanks for the quick replies guys :raisemyglass:

I should probably note that I do have a pressure cooker


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
Offlinemushpunx
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22390041 - 10/16/15 07:25 PM (8 years, 3 months ago)

Oh well then Right on man if you have a PC and have a print then you have the option of moving beyond PF tek, using agar to create clean innoculant and making clean grain spawn :thumbup:


--------------------

Amateur Mycologists United
AMU Q&A


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InvisibleKush_Zombie
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Registered: 10/22/14
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Re: Growing shrooms from spore print? [Re: mushpunx]
    #22390312 - 10/16/15 08:18 PM (8 years, 3 months ago)

Quote:

mushpunx said:
Oh well then Right on man if you have a PC and have a print then you have the option of moving beyond PF tek, using agar to create clean innoculant and making clean grain spawn :thumbup:



I heard to do grain to grain you had to have like some badass air filter in the room and a ridiculously sterile environment though :/
It's my first time ever growing so I'm not really educated on all of this that much tbh. I ordered a spore syringe, it's just that after I grow those mushrooms from that syringe I won't have any money to do much else other than take spore prints. Would I be able to re-use that syringe that I ordered by any chance?

P.S. this is the strain I ordered. They just made my mouth water when I saw them and I was like "Yep, that's the one."

Huautla Psilocybe Cubensis  :awesomenod:



--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
InvisibleKush_Zombie
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Registered: 10/22/14
Posts: 4,793
Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22390329 - 10/16/15 08:21 PM (8 years, 3 months ago)

Also, very random question, but I should pick them while the caps are still attatched to the stems right? They're more potent before they have a chance to show their spores aren't they? Hopefully that makes sense


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
InvisibleLocN9ne
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22390395 - 10/16/15 08:34 PM (8 years, 3 months ago)

Yes you can reuse a syringe, there's a few teks for that... as for harvesting...when the veil is breaking is a good time to pick to avoid a sporesplosion


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


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InvisibleKush_Zombie
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Registered: 10/22/14
Posts: 4,793
Re: Growing shrooms from spore print? [Re: LocN9ne]
    #22390415 - 10/16/15 08:37 PM (8 years, 3 months ago)

Quote:

LocN9ne said:
Yes you can reuse a syringe, there's a few teks for that... as for harvesting...when the veil is breaking is a good time to pick to avoid a sporesplosion



Thanks for answering my noob questions c:
I'll do more research when it gets closer to time, I'm just really tired today and thought it'd be easier to make a couple of posts. My brain needs a rest from the search bar


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
OfflineDarkhome
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22391137 - 10/16/15 10:36 PM (8 years, 3 months ago)

Quote:

Kush_Zombie said:
Quote:

mushpunx said:
Oh well then Right on man if you have a PC and have a print then you have the option of moving beyond PF tek, using agar to create clean innoculant and making clean grain spawn :thumbup:



I heard to do grain to grain you had to have like some badass air filter in the room and a ridiculously sterile environment though :/
It's my first time ever growing so I'm not really educated on all of this that much tbh. I ordered a spore syringe, it's just that after I grow those mushrooms from that syringe I won't have any money to do much else other than take spore prints. Would I be able to re-use that syringe that I ordered by any chance?

P.S. this is the strain I ordered. They just made my mouth water when I saw them and I was like "Yep, that's the one."

Huautla Psilocybe Cubensis  :awesomenod:





I've done Grain to Grain many many times now...All you need is a SAB, good sterile techniques, and of course the Master and receiving jars.
Grain to Grain is a great way to expand your Spawn!


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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Offlinespirit_shadow
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Re: Growing shrooms from spore print? [Re: Darkhome]
    #22391240 - 10/16/15 11:02 PM (8 years, 3 months ago)

print to agar- agar to clean transfer- clean plate to whatever you want :thumbup:


--------------------
ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011
Ban lotto


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Offlinemushpunx
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Registered: 04/20/14
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Re: Growing shrooms from spore print? [Re: spirit_shadow]
    #22394372 - 10/17/15 05:14 PM (8 years, 3 months ago)

Yea you are thinking of a laminar flow hood, like the one in my sig.

It pushes air through a HEPA filter at a special rate, so you have sterile air in laminar flow, giving you a certain amount of space to work in front of the hood.
The air coming out is sterile, but what's important is the laminar flow. It makes it so you can keep a sterile container sterile with the lid off.

A flow hood is super awesome and convenient but all it really does is give you elbow room. You can accomplish anything you would do in front of a hood in a still air box.

A still air box isn't sterile, it doesn't have to be. All it does is keep the air still so you can keep contaminants from landing in your work.

Both require practice and tip top sterile technique.

If you have a pressure cooker and still air box there is no limit to what you can grow at home!


--------------------

Amateur Mycologists United
AMU Q&A


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InvisibleKush_Zombie
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Registered: 10/22/14
Posts: 4,793
Re: Growing shrooms from spore print? [Re: mushpunx]
    #22394623 - 10/17/15 06:12 PM (8 years, 3 months ago)

My god you guys are motivating the crap out of me. I can make unlimited shrooms if I have a pressure cooker and an SAB? Am I dreaming?
I feel like I'm losing my virginity all over again (hopefully with more success though)
Thank you all so much, I'll be sure to come back and let you guys know how my grow goes!


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
InvisibleLocN9ne
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22394718 - 10/17/15 06:39 PM (8 years, 3 months ago)

With a pressure cooker, a sab, and sterile agar techniques...one could grow more mushrooms than one could eat from a single drop from a spore syringe...get to work boy


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
InvisibleKush_Zombie
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Registered: 10/22/14
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Re: Growing shrooms from spore print? [Re: LocN9ne]
    #22395024 - 10/17/15 07:48 PM (8 years, 3 months ago)

after looking at the agar teks my head hurts. I'm having a hard time even understanding the easy teks :confused: I've been reading a lot today and staring at my computer screen a lot too so that probably doesn't help...


--------------------
How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
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Loc: to the brain Flag
Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22395094 - 10/17/15 08:03 PM (8 years, 3 months ago)

Can you make jello? Do you own a PC? Do you shower regularly? If so, you can succeed at agar... stop being scared.


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


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InvisibleMad Season
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Re: Growing shrooms from spore print? [Re: LocN9ne]
    #22395217 - 10/17/15 08:22 PM (8 years, 3 months ago)

Quote:

Mad Season said:
:lol: agar is just as easy to do as LC. It's the exact same thing really. Whatever your recipe was, you can just add 2g of agar for every 100 ml.

Quote:

Mad Season said:
Quote:

hiperhiper said:
so what's the right procedure with grains?
I've checked the teks and nobody said anything about this. I didn't know I was not supposed to use syringe on grains. What is cleaning up syringe on agar? what does it mean?




http://www.shroomery.org/forums/showflat.php/Number/18431006
http://www.shroomery.org/forums/showflat.php/Number/18430998
http://www.shroomery.org/forums/showflat.php/Number/19208976 http://www.shroomery.org/forums/showflat.php/Number/21889950/page/1



All of those threads are amazing.




--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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Offlinemushpunx
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Re: Growing shrooms from spore print? [Re: Kush_Zombie]
    #22395260 - 10/17/15 08:32 PM (8 years, 3 months ago)

Quote:

Kush_Zombie said:
after looking at the agar teks my head hurts. I'm having a hard time even understanding the easy teks :confused: I've been reading a lot today and staring at my computer screen a lot too so that probably doesn't help...





Try watching letsgrowmushrooms by RR, great section on agar, pouring plates and culture work. Easier with a visual aid then read up


--------------------

Amateur Mycologists United
AMU Q&A


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InvisibleMad Season
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Re: Growing shrooms from spore print? [Re: mushpunx]
    #22395264 - 10/17/15 08:33 PM (8 years, 3 months ago)

:whathesaid: :werd: I might have to watch those videos one day


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
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