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Offlinejivangilad
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Can't air contaminate in Spore printing pictorial?
    #22388021 - 10/16/15 11:43 AM (8 years, 3 months ago)

According to this guide for making a print.
http://www.shroomery.org/8404/Spore-printing-pictorial
Air can enter under the glass, and therefore air born contamination can be caused. Isn't it?


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InvisibleDaveyJones6911
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Re: Can't air contaminate in Spore printing pictorial? [Re: jivangilad]
    #22388044 - 10/16/15 11:54 AM (8 years, 3 months ago)

i have made many prints this and similar ways and i never had a
spoiled print.


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Invisibleazur
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Re: Can't air contaminate in Spore printing pictorial? [Re: jivangilad]
    #22388054 - 10/16/15 11:58 AM (8 years, 3 months ago)

Quote:

jivangilad said:
According to this guide for making a print.
http://www.shroomery.org/8404/Spore-printing-pictorial
Air can enter under the glass, and therefore air born contamination can be caused. Isn't it?



That is why we use agar.


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InvisibleDaveyJones6911
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Re: Can't air contaminate in Spore printing pictorial? [Re: azur]
    #22388173 - 10/16/15 12:38 PM (8 years, 3 months ago)

Quote:

azur said:
Quote:

jivangilad said:
According to this guide for making a print.
http://www.shroomery.org/8404/Spore-printing-pictorial
Air can enter under the glass, and therefore air born contamination can be caused. Isn't it?



That is why we use agar.




i never used agar in the past.
i used to make my print, put it in a ziplock and then later stab
the baggie with a syringe, squirt in sterile water and suck it up again.

not to say that that is how it should be done, but i got away with it
a ton of times and that does indicate that these teks are cleaner then they look.


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InvisibleInocuole
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Re: Can't air contaminate in Spore printing pictorial? [Re: DaveyJones6911]
    #22388232 - 10/16/15 12:50 PM (8 years, 3 months ago)

Or that spores germinate faster than contams, indicates at least one of those things.


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Offlinejivangilad
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Re: Can't air contaminate in Spore printing pictorial? [Re: azur]
    #22388530 - 10/16/15 02:06 PM (8 years, 3 months ago)

Quote:

azur said:

That is why we use agar.



And with what do you inoculate it? with a piece of mushroom?


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InvisibleInocuole
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Re: Can't air contaminate in Spore printing pictorial? [Re: jivangilad]
    #22388537 - 10/16/15 02:07 PM (8 years, 3 months ago)

That or spores to agar, yes.


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InvisiblebodhisattaMDiscordReddit
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Re: Can't air contaminate in Spore printing pictorial? [Re: Inocuole]
    #22388595 - 10/16/15 02:18 PM (8 years, 3 months ago)

spore prints have more than billions of spores and if you do them clean enough maybe 1-100 CFU(colony forming units IE contaminants) chances are if you make a syringe you won't even pick one up. if you do chances are it won't even matter(unless you try inoculating a LC with it). unless you make your prints like a crackhead they're fine. worry about being super duper extra fucking clean with everything else in this hobby.


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Offlineblackout
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Re: Can't air contaminate in Spore printing pictorial? [Re: jivangilad]
    #22388601 - 10/16/15 02:20 PM (8 years, 3 months ago)

Quote:

jivangilad said:
Quote:

azur said:

That is why we use agar.



And with what do you inoculate it? with a piece of mushroom?



If you make a spore syringe you should suck up minimal amount of spores, this way if there are contaminants on the print its less likely they are sucked up. Then you drop a single drop of this well mixed syringe onto an agar plate, so again you have reduced the likelihood of contaminants.


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Re: Can't air contaminate in Spore printing pictorial? [Re: blackout]
    #22388621 - 10/16/15 02:23 PM (8 years, 3 months ago)

:bigyesnod::whathesaid:

a nice dark spore print will make hundreds of syringes.


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InvisiblebodhisattaMDiscordReddit
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Re: Can't air contaminate in Spore printing pictorial? [Re: bodhisatta]
    #22388647 - 10/16/15 02:29 PM (8 years, 3 months ago)



the fruits already grew in open air, no need to be extra overcautious.



I guarantee I'll have 0 problems with these prints.


Also,
http://www.shroomery.org/forums/showflat.php/Number/21365919#21365919


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Offlinemaryxmas
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Re: Can't air contaminate in Spore printing pictorial? [Re: bodhisatta]
    #22388763 - 10/16/15 02:55 PM (8 years, 3 months ago)

I usually swab down the surface I am going to do the prints on with 99% ISO alcohol.


Then wipe down the pre-cut foil pieces with ISO alcohol and place them down on the clean surface with latex gloves hands.

Then place two pieces of sterilized short glass rod on the foil and rest the mushroom cap onto the rods so that it doesn't contact the foil.

cover with a clean cup then cover the whole surface with a upside down Tupperware container to limit dust falling in the area.

12 hours later lift  the Tupperware and the  cup and remove the mushroom cap and rods gently so you don't disturb the print.

Then replace the cup over the print and use one of the rods to prop up the rim so it allows air and moisture exchange.  Replace the Tupperware container to limit dust settling.

After 24 hours remove the Tupperware container and cup and fold up your clean print.

Doing this method I have printed wild prints and had 100% success transferring to agar or straight to wbs via MS injection


Edited by maryxmas (10/16/15 02:59 PM)


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InvisibleInocuole
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Re: Can't air contaminate in Spore printing pictorial? [Re: maryxmas]
    #22388779 - 10/16/15 02:58 PM (8 years, 3 months ago)

Quote:

maryxmas said:
Then wipe down the pre-cut foil pieces for the pieces with ISO alcohol and my them down on the clean surface with latex gloves hands.




:justnotright:


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Offlinemaryxmas
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Re: Can't air contaminate in Spore printing pictorial? [Re: Inocuole]
    #22388800 - 10/16/15 03:01 PM (8 years, 3 months ago)

I let it evaporate before printing


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InvisibleInocuole
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Re: Can't air contaminate in Spore printing pictorial? [Re: maryxmas]
    #22388804 - 10/16/15 03:01 PM (8 years, 3 months ago)

Yeah but... I don't think iso can make foil any cleaner than it already is.  Doesn't kill mold spores. :shrug:

An bottle of iso opened enough times in open air likely has plenty of mold spores chilling in it waiting for a more suitable environment.  Probably not enough to fuck a print up, but still.


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Re: Can't air contaminate in Spore printing pictorial? [Re: maryxmas]
    #22388816 - 10/16/15 03:04 PM (8 years, 3 months ago)

Quote:

maryxmas said:
I usually swab down the surface I am going to do the prints on with 99% ISO alcohol.


Then wipe down the pre-cut foil pieces for the pieces with ISO alcohol and my them down on the clean surface with latex gloves hands.

Then place two piece of sterilized short glass rod on the foil and rest the mushroom cal onto the rods so that it doesn't contact the foil and cover with a clean cup then cover the whole surface with a upside down Tupperware container to limit dust falling in the area.

1: hours later lift  the Tupperware and the  cup and remove the cap and rods gentle so you don't disturb the print.

Then replace the cup over the print and use one of the rods to prop it up so it allows air and moisture exchange.  Replace the Tupperware container to limit dust settling.

After 24 hours remove the Tupperware container and cup and fold up your clean print.


Doing this method I have printed wild prints and had 100% success transferring to star or straight to wbs via MS injection



:facepalm:


70% iso is far superior as a sanitizer compared to 91-99%
if you buy 91% and want to use it as a sanitizer dilute it down
Quote:

bodhisatta said:
C1V1=C2V2 is the concentration calculation you can dilute it to make 70% with distilled water

(0.70)(650mL)=(.91)(500mL)

add 150ml of water to 500ml of 91% yields you 650mL of 70%.




alcohol is only a sanitizer
it does not sterilize

Quote:

I usually swab down the surface I am going to do the prints on with 99% ISO alcohol.



take prints on sterile surfaces not sanitized ones.  :homerdoh3:


Foil comes STERILE off the roll, if you wipe down foil with alcohol that's fucking stupid. alcohol is a sterilizer. wiping something sterile down with alcohol is like pissing on your hands after washing them.

if you don't obtain a sterile piece of foil since you cut it before taking a print, you can bake it to sterilize it, but it's best to just tear off a new sterile piece from the roll and then cut and fold it later on.

all this goes to show though is even if you take a print in a really shitty way it doesn't matter as long as it's pretty clean. I would do better job than that at taking a print though if I were you.


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Offlinemaryxmas
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Re: Can't air contaminate in Spore printing pictorial? [Re: bodhisatta]
    #22389605 - 10/16/15 05:48 PM (8 years, 3 months ago)

I'll take the cleanliness of my jug of pharmaceutical grade ISO alcohol over a roll of metal that comes in a cardboard box stored in a drawer.


And I don't use the alcohol to sterilize the foil..... But to clean it of any dust. The alcohol evaporates quickly (hence why I use 91% ISO) and leaves a dry clean surface with no residue.


Edited by maryxmas (10/16/15 06:01 PM)


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InvisibleInocuole
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Re: Can't air contaminate in Spore printing pictorial? [Re: maryxmas]
    #22389643 - 10/16/15 05:56 PM (8 years, 3 months ago)

As long as you're not disillusioned into thinking it's sterile, be my guest.


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Re: Can't air contaminate in Spore printing pictorial? [Re: maryxmas]
    #22389698 - 10/16/15 06:04 PM (8 years, 3 months ago)

Quote:

maryxmas said:
I'll take the cleanliness of my jug of pharmaceutical grade ISO alcohol over a roll of metal that comes in a cardboard box stored in a drawer.


And I don't use the alcohol to sterilize the foil..... But to clean it of any dust. The alcohol evaporates quickly (hence why I use 91% ISO) and leaves a dry clean surface with no residue.



:facepalm:

Plate 91% sometime on plate count agar. And incubate it in the 80-90f range.


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Re: Can't air contaminate in Spore printing pictorial? [Re: bodhisatta]
    #22389705 - 10/16/15 06:07 PM (8 years, 3 months ago)

Dust...?

I've never seen dust on foil in my life.


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