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GoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
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Mycelium enzyme production to dissolve food
#22386114 - 10/15/15 10:33 PM (8 years, 3 months ago) |
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Hello I'm just beginning to see the beauty of the fungi kingdom and was interested by the way mycelium digest nutrients I have read through the producing of 'environment' dissolving enzymes which break cells down into simpler compounds that the mycelium can digest. Any clarification on this subject would be greatly appreciated I hope to do an experiment in the next few weeks. Which brings me to my next point do environmental changes, i.e. temp. light. available nutrients, certain amino acids added to environment, etc. have noticeable effects on mycelium behavior, reproduction, enzyme production, anything. Just curious for some information on the subject like I said still very new to understanding the lives of fungi so ANY information is much appreciated. Thanks.
-------------------- Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money. But hell life's right in front of me, nothing to look forward to. Best of luck to all.
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GoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
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Re: Mycelium enzyme production to dissolve food [Re: GoldenGrowBoy]
#22389126 - 10/16/15 04:11 PM (8 years, 3 months ago) |
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Any information will really be helpful.
-------------------- Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money. But hell life's right in front of me, nothing to look forward to. Best of luck to all.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Mycelium enzyme production to dissolve food [Re: GoldenGrowBoy]
#22389160 - 10/16/15 04:15 PM (8 years, 3 months ago) |
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well, everything needs DNA and RNA
that is transcribed in the rhibosomes and proteins are packaged in the golgi apparatus >.>
the questions you are asking can be found in any college level biology book I believe
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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GoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
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Re: Mycelium enzyme production to dissolve food [Re: micro]
#22389927 - 10/16/15 07:02 PM (8 years, 3 months ago) |
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I was curious if the addition of certain lipids or carbohydrates or anything would have any effect on the way mycelium grows or digests nutrients.
-------------------- Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money. But hell life's right in front of me, nothing to look forward to. Best of luck to all.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Mycelium enzyme production to dissolve food [Re: GoldenGrowBoy]
#22391932 - 10/17/15 05:32 AM (8 years, 3 months ago) |
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What do you mean by "certain lipids or carbohydrates?" As in, what is the differentiating factor?
Saturated vs. unsaturated fatty acids? Simple vs. complex carbohydrates?
I would say it's niche is extremely complex carbohydrates, i.e. ones we can't even digest. Fungi has no problem breaking down cellulose but I'm sure simple sugars could be broken down, too. They will breed bacteria and yeast though, in any type of cultivation scenario. Same with fats though mushrooms have plenty of lipids themselves and seem to have no problems with self-canabolization :V
It's also going to differ between species, as well.
That is the best I can answer this.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
Edited by micro (10/17/15 05:44 AM)
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Mycelium enzyme production to dissolve food [Re: GoldenGrowBoy]
#22394762 - 10/17/15 06:50 PM (8 years, 3 months ago) |
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Fungi are a very diverse kingdom and therefore it's difficult to list details without knowing which species you are interested in.
All environmental factors cause a change in every aspect of the life cycle. Normally it is not very significant but temperature, humidity, nutrients, light, moisture level, etc. all effect the physiological changes.
Amino acids can normally be substituted:
Quote:
All fungi can use amino acids as a nitrogen source. Often they need to be supplied with only one amino acid such as glutamic acid or glutamine, and from this they can produce all the other essential amino acids by transamination reactions. The standard form of these reactions is shown below, using as an example, the production of alanine from glutamic acid, or vice versa
Fungal Biology 4th edition by Jim Deacon
Here are good starting books: (they can all be found through google for free as a pdf)
The Mushroom Cultivator by Paul Stamets
Growing Gourmet and Medicinal Mushrooms by Stamets
Mushrooms: Nutritional Value, Medicinal Effect, and Environmental Impact by shu-ting Chang and Philip G Miles
Fungal Biology by Jim Deacon
Introduction to fungi by john webster and roland weber
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Mycelium enzyme production to dissolve food [Re: Toadstool5]
#22397824 - 10/18/15 01:45 PM (8 years, 3 months ago) |
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interesting
makes a lot of sense though; they aren't very different at all
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Mycelium enzyme production to dissolve food [Re: micro]
#22399585 - 10/18/15 07:22 PM (8 years, 3 months ago) |
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I have seen a few publications where they only used l-glutamic acid as a nitrogen source for agar and it seemed to work fine. Fungi are really versatile suckers!
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Mycelium enzyme production to dissolve food [Re: Toadstool5]
#22400695 - 10/18/15 11:02 PM (8 years, 3 months ago) |
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-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Mycelium enzyme production to dissolve food [Re: micro]
#22405042 - 10/19/15 07:12 PM (8 years, 3 months ago) |
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Thats an awesome experiment! Thanks for the link 
I wonder how much of the difference is because of available nitrogen and how much is caused by other available nutrients?
Their plates seem to have a decent amount of sectoring and everyone on the shroomery stresses the importance of using monocultures for comparisons. Is it really that important to isolate completely?
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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GoldenGrowBoy
Sproutling


Registered: 10/01/15
Posts: 35
Loc: Austria
Last seen: 8 years, 2 months
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Re: Mycelium enzyme production to dissolve food [Re: Toadstool5]
#22405791 - 10/19/15 09:55 PM (8 years, 3 months ago) |
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Very interesting I wonder how they would reproduce together after being weaned on a specific source of nitrogen, but the metabolic pathways of fungi are very adaptable. So probably normally, I would still like to see it under a microscope.
-------------------- Hey I'm a new grower and new to mushrooming in general I hope to learn as much about mushrooms as I possibly can I may be going to graduate school for mycology in the future, but I'm 27 and need money. But hell life's right in front of me, nothing to look forward to. Best of luck to all.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Mycelium enzyme production to dissolve food [Re: GoldenGrowBoy]
#22406811 - 10/20/15 12:10 AM (8 years, 3 months ago) |
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see them reproduce? under a microscope?
well-a hey, whatever blows your hair back 
yeah fungi have some pretty whacky shit
like the serine hydrolase found in pestalotiopsis microspora from that other thread
i mean, they can deploymerize polyurethane and live off just that and some sunlight
that *is* kinda creepy
oldest thing on earth and they mutate so goddamned quickly nobody can ever prove it
(that last part isn't 100% true btw)
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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